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Fluorescence immunochromatography kit for quantitative detection of human epididymis secretory protein-4 and preparation method thereof

An epididymal secreted protein, fluorescence immunochromatography technology, applied in analytical materials, biological testing, biological material analysis and other directions, can solve the problems of inability to meet the needs of grassroots hospitals, long response time, need to wait, etc., to reduce non-specific adsorption, emission The effect of narrow spectrum and improved sensitivity

Inactive Publication Date: 2016-02-10
DEMAIJI BIOTECH BEIJING
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The Chinese invention patent application with the application number 201410164258.5 discloses a preparation method of a rapid diagnostic kit for ovarian cancer, which is mainly based on microtiter plates (such as 96-well plates, 384-well plates) for analysis. The sensitivity is high, but the operation is complicated and the reaction time is long. long and expensive
And if the sample size is relatively small, you need to wait, which cannot meet the needs of primary hospitals, including county-level and township hospitals.

Method used

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  • Fluorescence immunochromatography kit for quantitative detection of human epididymis secretory protein-4 and preparation method thereof
  • Fluorescence immunochromatography kit for quantitative detection of human epididymis secretory protein-4 and preparation method thereof
  • Fluorescence immunochromatography kit for quantitative detection of human epididymis secretory protein-4 and preparation method thereof

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Embodiment 1

[0039] Example 1: Modified antibody with fluorescent dye in a covalent cross-linking manner, and quantitative detection of human epididymis secretory protein-4 by Wash immunochromatography technology

[0040] 1) Coupling of fluorescent dyes and antibodies

[0041] Mix the fluorescent dye rhodamine with an emission wavelength of 665nm and the human epididymis secretory protein-4 monoclonal antibody of 1 mg / ml, react at room temperature for 3 hours, add 1 mol / L hydroxylamine hydrochloride to terminate the reaction, and separate and purify with a chromatographic column or a chromatographic column to obtain Fluorescent dye-modified human epididymis secretory protein-4 monoclonal antibody. Similarly, the fluorescent dye rhodamine-modified rabbit IgG (quality control molecule) was obtained. The fluorescence emission wavelength of the fluorescent dye modified human epididymis secretory protein-4 antibody and the fluorescent dye modified rabbit IgG are both 665nm.

[0042] 2) Constr...

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Abstract

The invention discloses a fluorescence immunochromatography kit for quantitatively detecting human epididymis secretory protein-4 by taking fluorescent dye as a marker. The fluorescence immunochromatography kit disclosed by the invention realizes fluorescence quantitative detection for the human epididymis secretory protein-4, has the advantages of being good in stability, wide in linear range, good in specificity, accurate to quantify, simple and quick, can be used for simultaneously detecting whole blood, blood serum and plasma samples, and is suitable for hospitals of various levels.

Description

technical field [0001] The invention relates to the field of clinical immunodiagnostic kits for cancer in the field of medical testing, in particular to a fluorescent immunochromatographic kit for quantitatively detecting human epididymis secretory protein-4. Background technique [0002] Ovarian cancer is a malignant tumor that occurs in the ovarian tissue and its pathogenesis has not yet been clarified. It is one of the common malignant tumors of female reproductive organs. Its incidence rate is only lower than that of cervical cancer and uterine body cancer, and its mortality rate is relatively high. The early clinical symptoms of ovarian cancer are not typical and objective, and it is difficult to distinguish them from benign ovarian tumors. There is a lack of effective methods for early diagnosis, and patients often miss the best time for treatment. Therefore, ovarian cancer has become one of the major diseases that seriously threaten women's health. [0003] Due to th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/68G01N2800/7028
Inventor 李道锋潘文东王新华
Owner DEMAIJI BIOTECH BEIJING
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