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Method for extracting ornithogalum caudatum saponin

A technology of perennial saponin and extraction method, which is applied in the field of extracting perennial saponin by using ultrasonic method combined with reverse micelle technology, can solve the problem of low extraction efficiency of perennial saponin, waste of resources, and less research on extraction and separation of plant small molecule active components. and other problems, to achieve the effect of high yield, improved purity, and improved yield

Active Publication Date: 2015-06-03
江苏科兴诺生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In my country, the research on reverse micellar extraction technology has developed rapidly, but there are still few studies on the extraction and separation of active ingredients of plant small molecules
[0005] In view of the low extraction efficiency and serious waste of resources for the conventional extraction methods for the saponins of Panniba sinensis, it is necessary to study and find new extraction methods for Panniba sinensis saponins in order to improve its yield and purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Wash and air-dry Dieffenbachia tiger eye, crush it into powder, then place it in an ultrasonic extraction tank, add 6 times the volume of 70% ethanol solution for heating and ultrasonic extraction, the time is 30 minutes, the oscillation frequency is 50KHz, and the temperature is 60°C. Extracted 3 times, combined the extracts, concentrated and evaporated to dryness under reduced pressure, dissolved the residue with water until the concentration of saponin was 0.20 mg / ml, and adjusted the pH to 4.5 to obtain crude saponin extract; add 2.5 g of anionic surfactant to 50 In a mixed solvent of isooctane and n-octanol with a volume ratio of 2:1 in ml, stir until uniform, then add 5 ml of 0.03 mol / L phosphate buffer, stir for 2 hours at 30°C, and let stand for 6 hours to obtain Reverse micellar system: Add the crude saponin extract into the reverse micellar system at a volume ratio of 1:1, stir at 30°C for 10 minutes, let stand to separate layers, the upper layer is the reverse...

Embodiment 2

[0021] Wash and air-dry Dieffenbachia tiger's eye, crush it into powder, then place it in an ultrasonic extraction tank, add 8 times the volume of 90% ethanol solution for heating and ultrasonic extraction, the time is 20 minutes, the oscillation frequency is 80KHz, and the temperature is 70°C. Extract 2 times, combine the extracts, concentrate and evaporate to dryness under reduced pressure, dissolve the residue with water until the concentration of saponin is 0.15mg / ml, and adjust the pH to 6.0 to obtain crude saponin extract; add 1.8g of anionic surfactant to 100ml In a mixed solvent of isooctane and n-octanol with a volume ratio of 4:1, stir until uniform, then add 5ml of 0.06mol / L phosphate buffer, stir at 40°C for 1.5 hours, and let it stand for 5 hours to obtain a reverse gum Beam system; add the saponin crude extract into the reverse micellar system at a volume ratio of 3:1, stir at 40°C for 5 minutes, let stand and separate layers, the upper layer is the reverse micell...

Embodiment 3

[0023] Wash and air-dry Dieffenbachia tiger eye, crush it into powder, then place it in an ultrasonic extraction tank, add 7 times the volume of 60% ethanol solution for heating and ultrasonic extraction, the time is 40 minutes, the oscillation frequency is 100KHz, and the temperature is 80°C. Extracted 4 times, combined the extracts, concentrated and evaporated to dryness under reduced pressure, dissolved the residue with water until the concentration of saponin was 0.35 mg / ml, and adjusted the pH to 5.0 to obtain crude saponin extract; 6.6 g of anionic surfactant was added to 100 ml In a mixed solvent of isooctane and n-octanol with a volume ratio of 3:1, stir until uniform, then add 5ml of 0.05mol / L phosphate buffer, stir at 30°C for 3 hours, and let it stand for 12 hours to obtain a reverse gum Bundle system; add the saponin crude extract into the reverse micellar system at a volume ratio of 1:1, stir at 30°C for 10 minutes, let stand and separate layers, the upper layer is...

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Abstract

The invention discloses a method for extracting ornithogalum caudatum saponin by combining an ultrasonic method with a reverse micelle technology. The method comprises the following steps: by taking ornithogalum caudatum as a raw material, ultrasonically extracting a crude saponin extract by using 60-90 percent of ethanol solution, drying by distillation, dissolving by using water, regulating the pH value to be 4.5-6.0, extracting by using a reverse micelle system formed by a mixed solvent of isooctane and n-caprylic alcohol and phosphate buffer, mixing the obtained reverse micelle phase with the phosphate buffer, respectively adding ethyl acetate and chloroform for extracting, drying by distillation, thereby obtaining the product. The method disclosed by the invention has the advantages of high selectivity, mild conditions and high yield and purity.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to a method for extracting saponins of Divinia sinensis by using an ultrasonic method combined with reverse micellar technology. Background technique [0002] Ornithogalum caudatum (Ornithogalum caudatum), also known as pearl grass, cucurbit orchid, and evergreen, is a perennial herb of the genus Ornithogalum caudatum in the family Liliaceae. Tiger eye evergreen is sweet in taste and slightly cold in nature, and it returns to the liver and spleen meridian. It has the function of clearing away heat, detoxifying, eliminating stagnation and stagnation. Folks use its fresh juice to smear the affected area to treat sores. Oral administration can treat unknown swelling, hepatitis, liver cirrhosis, Liver cancer, etc., all have good therapeutic effects. Dieffenbachia tiger's eye has extremely high medicinal value, and its chemical components include saponins, polysa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/896
Inventor 巫军丁萍月张亚琼张佳佳王忠华
Owner 江苏科兴诺生物技术有限公司
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