Method for promoting aerobic respiration of lactococcus lactis

A technology of Lactococcus lactis and aerobic respiration, applied in the direction of using microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve problems such as damage, unretrieved, and unfavorable human health, and achieve simple operation, Low cost and strong practical effect

Inactive Publication Date: 2015-06-03
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, if heme is added directly in the production of dairy products, it is inevitable that some heme that has not been absorbed and utilized by cells will remain in dairy products, and a large amount of free heme will affect the kidneys and other organs through

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  • Method for promoting aerobic respiration of lactococcus lactis
  • Method for promoting aerobic respiration of lactococcus lactis
  • Method for promoting aerobic respiration of lactococcus lactis

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[0051] Examples:

[0052] In this example, the Lactococcus lactis subsp. crema was purchased from the China Common Microbial Species Collection Management Center, CGMCC number: 1.3992, genus name Lactococcus, isolation number 100676.

[0053] A method for promoting aerobic respiration of Lactococcus lactis subsp. cremoris, and its steps are as follows:

[0054] 1. Air elimination of the fermenter for 1h.

[0055] 2. Preparation of CM liquid medium: the specific method is to weigh 15g of yeast powder, 45g of soy protein, 45g of glucose, 1.5g of VC, and MgSO 4 .7H 2 O 1.5g, heated and dissolved in 3000ml distilled water, adjusted the pH to 6.5±0.1, autoclaved at 121°C for 15 minutes to obtain a CM liquid medium.

[0056] 3. Inoculation: Inoculate the activated Lactococcus lactis subsp. cremoris in the CM medium at an amount of 5%, and cultivate it to the logarithmic phase under the conditions of 30°C and 150 rpm as the seed solution.

[0057] 4. Cultivation: Inoculate the seed solution wit...

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Abstract

The invention belongs to the field of facultative anaerobic type microbial aerobic respiration and particularly discloses a method for promoting aerobic respiration of lactococcus lactis. The method comprises the following steps: inoculating activated lactococcus lactis seed solution into a culture medium; cultivating the lactococcus lactis at 30+/-5 DEG C, and feeding a NaOH solution to the culture medium in the culture process so that the pH value of the culture medium is constantly 6.5+/-0.1; and simultaneously introducing sterile air into the culture medium. The method has an obvious effect of promoting aerobic respiration of lactococcus lactis; the effect of promoting aerobic respiration is better than that by adding heme; and the method can be used for replacing an existing method in which heme is used. Meanwhile, the acidic stress tolerance and the low-temperature tolerance of lactococcus lactis can also be enhanced; the operation method is simple and relatively low in cost; and industrialized production is easy to realize.

Description

technical field [0001] The invention relates to the field of promoting the aerobic respiration of facultative anaerobic microorganisms, in particular to a method for promoting the aerobic respiration of Lactococcus lactis. Background technique [0002] At present, Lactococcus lactis is widely used in the production of fermented milk products, and it also has important application value in the expression of exogenous genes and live bacterial vaccine products. At the same time, it is also a model species of Gram-positive bacteria, which has important research value. [0003] Existing studies have shown that Lactococcus lactis is a facultative anaerobic bacteria and lacks a complete respiratory chain in the cell. Only when exogenous heme exists can its energy production mode change from fermentation to aerobic respiration. Under aerobic respiration, Lactococcus lactis produced more, and its biomass and long-term survival rate at 4°C were better than those without heme. The pr...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/20C12R1/01
CPCC12N1/36
Inventor 付瑞燕卫兰兰刘立明
Owner ANHUI AGRICULTURAL UNIVERSITY
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