Method for producing livestock feed ingredient using cellulase treatment of tobacco waste

A technology of tobacco waste and cellulase, which is applied in animal feed, animal feed, application and other directions, can solve the problems of unutilized, pollution, waste, etc., and achieves the improvement of effective utilization, safety, and effect. Effect

Inactive Publication Date: 2015-06-10
CHINA TOBACCO CHUANYU IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tobacco plants go through the seedling stage, cluster stage, flourishing stage and mature stage throughout their life. In addition to the tobacco leaves with industrial applicability, other parts of the tobacco plant, such as tobacco stems, tobacco roots, fireworks, tobacco branches, axillary buds, and leaves are removed. As tobacco waste, the top leaves and foot leaves are discarded in large quantities in the field, which not only causes pollution, but also is a huge waste

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0036] Preparation of re-screening medium: K 2 HPO 4 1.0g, MgSO 4 0.5g, 0.1% MnSO 4 Solution 1ml, concentration 0.1% FeSO 4 Solution 3ml, agar 1.5g and water to a constant volume of 1L, pH 6.0, sterilized at 121°C for 30min, after cooling, add nicotine 2000mg to obtain re-screening medium;

[0037] Seed culture medium: 3.0g of beef extract, 5.0g of peptone, and 3.0g of NaCl were adjusted to 1L of water, pH 7.0, and sterilized at 121°C for 30min.

[0038] Fermentation medium: Put 50g of tobacco leaves in a Erlenmeyer flask and sterilize at 121°C for 30min.

[0039]In July, 10g of tobacco field soil was collected at the Taiping Tobacco Leaf Base in Fengdu, Chongqing, and added to 100ml enrichment medium, and cultured at 30°C for 48 hours to obtain the enrichment medium, and then 1ml of the enrichment medium was inoculated in a 50°C primary screen In the culture dish of the medium, mix evenly, after solidification, put it in a constant temperature incubator at 30°C for 7 day...

Embodiment 1

[0041] Prepare 100 kg of tobacco rods discarded in Daejeon with a pulverizer into particles with a uniform size and a diameter of about 0.8 cm; the concentration of the cultured bacteria reaches 10 10 The Bacillus strain with nornicotine effect of cfu / ml is sprayed on the surface of the tobacco rod particle powder according to the addition ratio of 8%; at the same time, the cellulase powder (produced by Ningxia Xiasheng Industrial Group Co., Ltd.) is sprayed according to the ratio of 1:150 The mass ratio is mixed with water, and sprayed on the surface of the tobacco rod particles that have been sprayed with the bacterial liquid according to the addition ratio of 20% of the mass of the tobacco rod particles. During the spraying process, try to turn over the pile to ensure uniform spraying; then The tobacco rod particles are piled up in a shaded and well-drained corner. After 5 days, the fermentation liquid is drained; the fermented tobacco rod powder is added to the common feed ...

Embodiment 2

[0043] Prepare 100 kg of tobacco rods discarded in Daejeon with a pulverizer into particles with a uniform size and a diameter of about 0.8 cm; the concentration of the cultured bacteria reaches 10 9 The Bacillus strain with nornicotine effect of cfu / ml is sprayed on the surface of the tobacco rod particle powder according to the addition ratio of 8%; the cellulase powder (produced by Ningxia Xiasheng Industrial Group Co., Ltd.) according to the quality of 1:80 Mix it with water, and spray it on the surface of the tobacco rod particles that have been sprayed with the bacterial liquid according to the addition ratio of 15% of the mass of the tobacco rod particles. During the spraying process, turn the pile as much as possible to ensure uniform spraying; The powdered tobacco rods are stacked in a shaded and well-drained corner, and after 4 days, the fermentation liquid is drained; the fermented tobacco rod powders are added to the feed commonly used by sheep at a ratio of 16%, an...

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Abstract

The invention discloses a method for producing livestock feed ingredients using cellulase treatment of tobacco wastes. At first, tobacco stems are smashed; then cellulase solutions are sprayed, and the mass of the cellulase solutions is 5%-25% of the mass of tobacco stem powder; then bacillus bacteria liquid with a nornicotine function is sprayed, and the mass of the bacillus bacteria liquid is 5%-10% of the mass of the tobacoo stem powder; or the cellulase solutions and the bacillus bacteria liquid are sprayed simultaneously; after the cellulase solutions and the bacillus bacteria liquid are mixed uniformly and stacked in a shade and well drained corner for 3-5 days, the feed ingredients are obtained; then the feed ingredients are added to livestock feed at a ratio range of 10%-30% of the livestock feed mass to feed livestock. The method for producing the livestock feed ingredients using the cellulase treatment of the tobacco wastes makes full use of the tobacco wastes, uses the cellulase to degrade the cellulose part in the tobacco stems to cellobiose and glucose and uses nornicotined microorganisms to degrade the nicotine in tobacco leaves, the obtained feed ingredients are added to the normal feed at the ratio range of 10%-30% of the livestock feed mass to feed the livestock, the livestock weight of a control group increases by an average of 5%-10% of the livestock weight of a blank group, and the economic cost is saved by 8%-25%.

Description

technical field [0001] Embodiments of the present invention relate to the technical field of tobacco field waste recovery and reuse, and more specifically, embodiments of the present invention relate to a method of using cellulase to treat tobacco waste to produce livestock feed ingredients. Background technique [0002] my country is the country with the highest flue-cured tobacco production and the largest planting area in the world. In 2014, the national allocation of flue-cured tobacco raw tobacco reached 41 million tons (one load is 50 kg), and the planting area was about 20 million mu. Tobacco plants go through the seedling stage, cluster stage, flourishing stage and mature stage throughout their life. In addition to the tobacco leaves with industrial applicability, other parts of the tobacco plant, such as tobacco stems, tobacco roots, fireworks, tobacco branches, axillary buds, and leaves are removed. As tobacco waste, the top leaves and foot leaves are discarded in ...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23K1/00
Inventor 寇明钰戴亚施丰成肖克毅薛芳谭兰兰沈怡郭刚
Owner CHINA TOBACCO CHUANYU IND
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