Production technology for extracting eucommia flavonoid from eucommia ulmoides leaves by combining compound enzyme with ultrasound

A technology of Eucommia flavonoids and production process, which is applied in the directions of medical preparations containing active ingredients, food science, plant raw materials, etc., can solve the problems of backward production methods, high equipment costs and high operating temperature, and achieves less impurity content and extraction speed. Fast, easy-to-purify results

Inactive Publication Date: 2015-06-10
BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Currently used water extraction, two-phase system extraction, microwave extraction and other methods, the extraction and refining production methods are still relatively backward
There are obvious deficiencies in these conventional methods, such as the water extraction method, due to the high operating temperature, the eucommia flavonoids in Eucommia are prone to decomposition; ultrasonic extraction, microwave extraction, high equipment costs, new processes are not yet mature, and are not suitable for industrial production

Method used

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  • Production technology for extracting eucommia flavonoid from eucommia ulmoides leaves by combining compound enzyme with ultrasound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Weigh 500g of dried leaves of Eucommia ulmoides, crush them, pass through 50 mesh, add water to the powder and soak for half an hour; add 1.2g of cellulase and 3.9g of pectinase to it; adjust the pH to 5.0 with hydrochloric acid solution, and enzymatically hydrolyze at 49°C for 3h , and then boiled for 4min to inactivate the enzyme; add ethanol to the mixed solution until the concentration of ethanol is 50wt%, the ratio of solid to liquid is 1:42 (volume ratio, the same below), ultrasonic extraction for 22min, the temperature is 95 ℃, the power was 76%, the ultrasonic product was filtered, the filtrate was concentrated, and the pH was adjusted to 6.5 to obtain an extract.

[0020] Slowly add the above extract to the D-101 macroporous adsorption resin, and add the sample to 1 / 3 of the column. Water and ethanol with a concentration of 10wt% were selected for elution in sequence, and the eluent was discarded. Then elute with ethanol with a concentration of 90wt%, collect ...

Embodiment 2

[0022] Weigh 500g of dried leaves of Eucommia ulmoides, crush them, pass through 50 mesh, add water to the powder and soak for half an hour; add 0.6g of cellulase and 2.4g of pectinase to it; adjust the pH to 4.0 with hydrochloric acid solution, and enzymatically hydrolyze at 45°C for 2h , and then boiled for 2 minutes to inactivate the enzyme; add ethanol to the mixed solution until the concentration of ethanol is 50wt%, the ratio of solid to liquid is 1:40, ultrasonic leaching for 15min, the temperature is 90°C, the power is 70%, and the ultrasonic product is filtered , the filtrate was concentrated, and the pH was adjusted to 6.2 to obtain an extract.

[0023] Slowly add the above extract to the D-101 macroporous adsorption resin, and add the sample to 1 / 3 of the column. Water and ethanol with a concentration of 10wt% were selected for elution in sequence, and the eluent was discarded. Then elute with ethanol with a concentration of 90wt%, collect the eluent, concentrate, ...

Embodiment 3

[0025] Weigh 500g of dried leaves of Eucommia ulmoides, crush them, pass through 50 mesh, add water to the powder and soak for half an hour; add 1.2g of cellulase and 4.8g of pectinase to it; adjust the pH to 5.0 with hydrochloric acid solution, and enzymatically hydrolyze at 55°C for 3h , and then boiled for 4 minutes to inactivate the enzyme; add ethanol to the mixed solution until the concentration of ethanol is 50wt%, the ratio of solid to liquid is 1:45, ultrasonic extraction is 25min, the temperature is 100°C, the power is 80%, and the ultrasonic product is filtered , the filtrate was concentrated, and the pH was adjusted to 6.5 to obtain an extract.

[0026] Slowly add the above extract to the AB-8 macroporous adsorption resin, and add the sample to 1 / 3 of the column. Water and ethanol with a concentration of 10wt% were selected for elution in sequence, and the eluent was discarded. Then elute with ethanol with a concentration of 90wt%, collect the eluent, concentrate,...

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Abstract

The invention discloses a production technology for extracting eucommia flavonoid from eucommia ulmoides leaves by combining compound enzyme with ultrasound. The technology comprises the following steps: (1) crushing eucommia ulmoides leaves, filtering through a 50-mesh sieve, adding 3-5fold water to powder, soaking for half an hour, and adding cellulase and pectinase; (2) adjusting the pH to be 4.0-5.0 with a hydrochloric acid solution, carrying out enzymolysis at 45-55 DEG C for 2-3 hours, and boiling for 2-4 minutes to deactivate enzyme; (3) adding ethanol to the mixed liquid until the concentration of ethanol is 50wt%, carrying out ultrasonic extraction for 15-25 minutes at 90-100 DEG C and the power of 70%-80%, filtering an ultrasonic product, concentrating filtrate, and adjusting the pH to be 6.5, so as to obtain an extract liquid; and (4) separating the extract liquid with macroreticular resin, sequentially eluting with water and ethanol with the concentration of 10wt%, removing eluate, eluting with ethanol with the concentration of 90wt%, collecting the eluate and concentrating. The production technology is simple, feasible, low in cost, economical, and environment-friendly, and can be applied to industrialized production; and the effective substances in eucommia ulmoides can be extracted to the maximal extent.

Description

technical field [0001] The invention relates to a production process for extracting eucommia flavonoids from eucommia leaves in combination with complex enzymes and ultrasound, and belongs to the field of traditional Chinese medicine pharmacy or raw material processing. Background technique [0002] Eucommia ulmoides is a kind of Chinese herbal medicine with a wide range of uses. Flavonoids are one of the main active ingredients of Eucommia ulmoides, and their content is an important indicator for judging the quality of eucommia ulmoides crude drugs and their products. , quercetin, astragalin, terrestrial glycosides, rutin, etc. Clinical and pharmacological studies have shown that eucommia flavonoids have various pharmacological effects such as anti-cancer, anti-aging, lowering blood pressure, antibacterial and anti-inflammatory, and enhancing body immunity. They are widely used in the research and development of medicines and curative foods. There are a wide range of uses ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/46A61K8/97A23L1/30A61K127/00A23L33/105
Inventor 葛喜珍李丽娟
Owner BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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