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Cell culturing member, and cell culturing method

A cell culture, cell technology, applied in general culture methods, cell culture active agents, cell culture supports/coatings, etc., can solve the problem that three-dimensional cell arrangement cannot be controlled artificially

Inactive Publication Date: 2015-06-10
NISSHA PRINTING COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this methodology, the three-dimensional cell arrangement cannot be artificially controlled

Method used

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  • Cell culturing member, and cell culturing method
  • Cell culturing member, and cell culturing method
  • Cell culturing member, and cell culturing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080]

[0081] On a hot plate (50° C.), an agarose aqueous solution (80° C., 5%) was bar-coated on a PET film (TEIJIN Tetron Film G274) subjected to an easy-adhesive treatment. In an oven, heat drying was performed at 40° C. for 30 minutes.

[0082] A 30% gelatin aqueous solution was applied to a glass plate while heating it on a hot plate (50° C.). After cooling, the gelatin was cut out and mounted on the above-mentioned agarose-coated film. Thereafter, the film was slit to obtain a gelatin pattern substrate.

[0083] The prepared gelatin pattern substrate was heated and dried under vacuum at 140° C. for 48 hours to cross-link and sterilize the gelatin. The gelatin layer thickness as the hydrogel site was 50 μm, the gelatin pattern was one straight line, and the line width was 70 μm. The prepared gelatin pattern substrate was heated and dried at 140° C. for 48 hours in a vacuum to crosslink the gelatin and sterilize the substrate.

[0084] Cell growth factor HumanVEGF1...

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Abstract

[Problem] To address the problem of obtaining a cell culturing member which enables a structure of a cell culturing assembly to be controlled in three dimensional directions. [Solution] A cell culturing member (1) in which a patterned and layered hydrogel part (12), and a layered culturing part (13) which covers the hydrogel part (12) are formed on a substrate (11). A surface of the culturing part is a first cell seeding surface (14). The hydrogel part (12) is permeated by a cell growth factor, and the thickness of the hydrogel part enables a controlled release of the cell growth factor from the hydrogel part (12) to the culturing part (13) during the culturing period.

Description

technical field [0001] The present invention relates to a cell culture member for in vitro cell culture of humans, animals, and the like. In addition, the present invention relates to a cell culture method for culturing cells in vitro. Background technique [0002] In a living body, cells have a certain regular, three-dimensional arrangement and configuration. However, in current cell culture, techniques related to the arrangement and arrangement of the cells are extremely limited. [0003] Cell arrangements involving two dimensions have been reported so far. For example, two-dimensional adhesion of cells is performed by forming a pattern of a cell-adhesive surface and a non-adhesive surface, followed by controlling a proliferation pattern. As an example, conventionally known are: a cell culture support in which a temperature-sensitive polymer is patterned with a fine line width on a substrate, and a cell culture method for culturing cells using the cell culture support (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/04C12N1/00C12N5/071
CPCC12M25/06C12N5/069C12N2501/165C12N2533/10C12N2533/12C12N2533/54C12N2533/30C12N2537/10C12N5/0068
Inventor 田畑泰彦小尾奈绪子住吉千夏子村上英树
Owner NISSHA PRINTING COMPANY