Method for preparing anti-oxidation active liquid by performing enzymatic hydrolysis on soft-shelled turtle eggs

A technology of antioxidant activity and soft-shelled turtle eggs, applied in food preparation, hydrolyzed protein ingredients, cosmetics, etc., can solve the problems of waste, processing and discarding soft-shelled turtle eggs, etc., and achieve the effects of stable hydrolyzed components, no pollution to the environment, and easy absorption

Inactive Publication Date: 2015-06-17
杨明毅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, unfertilized soft-shelled turtle eggs are generally processed or discarded as food raw materials, causing a large d

Method used

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Examples

Experimental program
Comparison scheme
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Example Embodiment

[0017] Example 1:

[0018] (1) Reverse osmosis water cleaning: Use reverse osmosis water to clean turtle eggs. The reverse osmosis water is made from the general reverse osmosis water equipment in the market, and the conductivity is less than 5 s / cm;

[0019] (2) Stirring: Put the soft-shelled turtle eggs into the bioreactor, add deionized water 15 times the weight of soft-shelled turtle eggs, add biological enzymes for enzymatic reaction, and add biological enzymes into four types: 1. Neutral Protease is a proteolytic enzyme extracted from the fermentation of Bacillus subtilis. The suitable temperature is 35-55℃, the pH is 6-7.5, the activity is 100,000 units per gram, the addition amount is 0.05-0.25%, and the action time is 2-3 hours. The enzymatic reaction of protease hydrolyzes the large-molecule protein of turtle eggs into small-molecule peptides and amino acids; 2. Special enzyme for egg white and yolk, suitable temperature 50-55℃, PH7-8, action time 2-3 hours, addition amou...

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Abstract

The invention discloses a method for preparing anti-oxidation active liquid by performing enzymatic hydrolysis on soft-shelled turtle eggs. The method comprises the following steps: reverse osmosis water washing, stirring, passivation, filtration, centrifugation, concentration, membrane filtration, filling and sealing. According to the technical scheme of the method, soft-shelled turtle egg protein is mildly hydrolyzed by using a modern biological enzyme engineering technology, hydrolysis components are relatively stable, environmental pollution cannot be caused, and the method is easy to operate and control; by controlling hydrolysis conditions, nutritional values of the anti-oxidation active liquid can be improved and the anti-oxidation active liquid can be easily absorbed; moreover, the mixed liquid has a certain antioxidant capacity, and the antioxidant activity of the mixed liquid cannot be reduced after the mixed liquid is heated for 30 minutes ( by virtue of pasteurization) in hot water of which the pH value is 4.0 and the temperature is 85 DEG C; and the mixed liquid of amino acids, peptides and relatively large-molecular protein, prepared by using the method disclosed by the invention, have antioxidant capacity and thermal stability and can be applied to the field of processing of cosmetics, health products and foods.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing an anti-oxidation active liquid by enzymatically hydrolyzing soft-shelled turtle eggs. Background technique [0002] The general working procedures of soft-shelled turtle farming enterprises are as follows: from May to August, soft-shelled turtles start to lay eggs, and after soft-shelled turtle eggs are produced, they are selected to distinguish fertilized eggs from unfertilized eggs. The fertilized eggs continue to hatch until the soft-shelled turtles are hatched, and the soft-shelled turtles are sold or continue to grow up to sell finished products. And at present, unfertilized soft-shelled turtle eggs are generally processed or discarded as food raw materials, causing waste to a large extent. Antioxidants refer to substances that can effectively inhibit the oxidation reaction of free radicals when they exist in low concentrations, and eliminating the poiso...

Claims

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Application Information

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IPC IPC(8): A61K38/01A61K35/586A61K8/98A61K8/64A61P39/06A61Q19/08A23L1/305A23L33/17
Inventor 杨明毅高丹文
Owner 杨明毅
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