Recombinant vector, recombinant baculovirus prepared with the same and application of virus in preparation of malaria vaccines

A technology of recombinant baculovirus and recombinant vector, which is applied in the field of biomedical technology and can solve the problems of high cost, low expression amount, unsuitable for production needs and the like
CN104711289AInactive Publication Date: 2015-06-17特菲(天津)生物医药科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
特菲(天津)生物医药科技有限公司
Publication Date
2015-06-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a recombinant vector, a recombinant baculovirus prepared with the vector and application of the virus in preparation of malaria vaccines. The recombinant vector is constructed by inserting a section of recombinant sequence into the pFastBacDual vector. The recombinant sequence is formed by: according to CMV, Ph, SP and TM sequences, adding EcoR I enzyme site and Xho I enzyme site between SP and TM, adding KpnI enzyme site in front of CMV-F, and adding HindIII enzyme site after TM-R. The recombinant baculovirus is obtained by: inserting Plasmodium yoelii Pys25 antigen gene into the recombinant vector, conducting homologous recombination with the genome of a shuttle vector Bacmid through transposition, then transfecting a bombyx mori cell, and performing packaging in the bombyx mori cell. Through simple separation and purification of the recombinant baculovirus, an antibody with good titer can be prepared, thus providing a reference for study of P25 malaria vaccines.
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Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and specifically relates to a modified baculovirus vector, that is, a recombinant vector. A recombinant baculovirus containing a target gene (Plasmodium yoelii surface protein Pys25 gene) prepared by using the vector, the recombinant baculovirus The preparation method of the baculovirus, the fusion protein expressed by the recombinant baculovirus, and the application of the recombinant baculovirus and the fusion protein in the preparation of malaria vaccine. Background technique

[0002] Malaria transmitted by vector mosquitoes is an infectious disease that seriously threatens human life and health. According to the World Malaria Report 2011 published by WHO in 2011, in 2010, a total of 216 million medical records were found in 106 malaria-endemic countries and regions around the world, of which 86% were children under the age of 5. It is estimated that in 2010, there were more than 650,000 ...

Claims

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