Multi-parameter nanopore monomolecular analyzer with alternating-current mode

Abstract

The invention discloses a multi-parameter nanopore monomolecular analyzer with an alternating-current mode. The multi-parameter nanopore monomolecular analyzer comprises a nanopore detection pool, wherein two electrodes of the nanopore detection pool are connected with a probe of a current amplifying system; the output of an alternating-current signal generation system is connected with the input of the current amplifying system; the potential output of the current amplifying system is connected with a potential inlet of a phase-locked amplifying system; the current output of the current amplifying system is connected with a current inlet of the phase-locked amplifying system by a filtering system; and a potential outlet, a current outlet, a vibration-amplitude outlet and a phase outlet of the phase-locked amplifying system are respectively connected with corresponding interfaces of a multi-channel data synchronous-acquisition system which is connected with a display and analysis system of a computer. The multi-parameter nanopore monomolecular analyzer disclosed by the invention has the advantages that many parameters such as direct-current component current, alternating-current component vibration amplitude, phase difference and impedance change caused by single molecules penetrating through the nano pores can be detected, so that the selectivity for nanopore measurement can be improved, and the multi-parameter nanopore monomolecular analyzer can be applied in screening and analytic detection of multiple biological macromolecules and medicine molecules.

Description

technical field [0001] The invention relates to the technical field of nanopore single-molecule analysis, in particular to a multi-parameter nanopore single-molecule analyzer in an AC mode. Background technique [0002] Nanopore single-molecule analysis technology is an analysis method established by applying a constant potential to measure the change of ion current when the target passes through the nanopore channel. At present, the widely used nanoporous materials are: α-hemolysin, MspA and phi29-based protein nanopores (1-3 nanometers in diameter) and inorganic materials (silicon and silicon nitride, alumina, glass, etc.), Artificial solid nanochannels (tens to hundreds of nanometers in diameter) such as polymers, carbon nanotubes, and graphene. Nanopore technology has great application value in DNA sequencing, single-molecule detection and single-molecule chemical reaction mechanism research, and its application range covers many fields such as chemistry, biology, medic...

AI Technical Summary

Problems solved by technology

[0004] However, the nanopore current change signal brought by the applied constant potential measurement is single, has low selectivity and poor sensitivity to the analyte, and cannot provide enough rich information to study the conformational change and interaction mechanism of the analyte during the perforation process. Limits the application of nanopore technology

Nanopore measurements in potentiostatic mode have unavoidable technical limitations

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