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Construction method of mouse model of hepatitis B virus infection combined with alcoholic fatty liver

A technology of hepatitis B virus and alcoholic fatty liver, which is applied in the field of establishing mouse models of chronic hepatitis B virus infection combined with alcoholic fatty liver, can solve the problems of various influencing factors and adverse effects research, and achieve low-cost, repeatable The effect of good sex and clear genetic background

Active Publication Date: 2019-02-01
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods can effectively establish the model of alcoholic liver injury, there are many influencing factors, which are not conducive to the study of the effect of alcohol single factor on alcoholic liver disease.
[0006] At present, there are only animal models for simple HBV infection and pure alcoholic fatty liver, but there is no animal model for combining HBV infection and alcoholic fatty liver. A mouse model of alcoholic fatty liver, the mouse model established by this method is cheap and easy to reproduce, with obvious clinical symptoms and similar to human conditions

Method used

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  • Construction method of mouse model of hepatitis B virus infection combined with alcoholic fatty liver
  • Construction method of mouse model of hepatitis B virus infection combined with alcoholic fatty liver
  • Construction method of mouse model of hepatitis B virus infection combined with alcoholic fatty liver

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Experimental program
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Effect test

Embodiment 1

[0054] Embodiment 1 Experimental animal and material

[0055] 1. Animals

[0056] FVB / N mice (6-8 weeks, SPF grade, male) were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.

[0057] 2. Plasmid

[0058] The pGEM4Z / HBV1.3 plasmid was provided by the Institute of Microbiology and Immunology, Yang Ming University, Taiwan.

[0059] The control plasmid pGEM4Z was purchased from Wuhan Promega Biotechnology Company.

[0060] 3. Feed

[0061] AIN93 alcohol liquid feed and AIN93 control liquid feed were purchased from Jiangsu Nantong Trofee Company.

[0062] AIN93 alcoholic liquid feed: 120g of main ingredients, 2.5g of vitamins, 8.7g of minerals, edible alcohol (95%) of 1-5% (v / v), appropriate amount of distilled water.

[0063] During use, according to the needs of different alcohol concentration of liquid feed, add edible alcohol (concentration of edible alcohol is 95%) in main feed material, vitamin, mineral matter respectively, then add distille...

Embodiment 4

[0110] The conventional alcohol induction feeding of embodiment 4 and comparative test of the present invention's method

[0111] 1. Grouping of mice

[0112] FVB / N mice were randomly divided into four groups, that is, pure drinking control group A and B; chronic hepatitis B virus infection combined with alcoholic fatty liver model group C and D, 10 in each group;

[0113] 2. Virus infection

[0114] 2-1. Dissolve the control plasmid pGEM4Z (10 μg) in 0.01 mol / L phosphate buffer solution (PBS, pH 7.4) to prepare a control plasmid pGEM4Z solution, wherein the weight of the PBS buffer solution is equal to the weight of the mouse The ratio was 8:100; then the control plasmid pGEM4Z solution was injected into the mice of groups A and B of the drinking group A and B by high pressure through the tail vein, and the composition of the PBS buffer solution was NaCl 8.0g, KCl 0.2g, KH 2 PO 4 0.24g, Na 2 HPO 4· 12H 2 O3.63g, dilute to 1000ml, adjust pH=7.4;

[0115] 2-2. Dissolving...

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Abstract

The invention discloses a construction method of a mouse model for simulating the chronic hepatitis B virus (HBV) infection and the alcoholic fatty liver. A pGEM4Z / HBV1.3 plasmid is injected to the body of an FVB / N mouse through the caudal vein at a high pressure so that the HBV duplicates slowly in the liver of the mouse, then the mouse is fed with an alcohol-containing liquid feed for more than 5 weeks, and the changes of aspartate aminotransferase (AST), alanine transaminase (ALT) and hepatitis B e antigen (HBeAg) in the liver tissues and serum of the mouse are observed, so that the mouse model for simulating the pathological process of the human chronic HBV infection and the alcoholic fatty liver is constructed. The constructed mouse model is a powerful tool for systematically researching the genetic regulation, molecular mechanism and control measure of the chronic HBV infection and the alcoholic fatty liver.

Description

technical field [0001] The invention relates to a method for establishing a mouse model of chronic hepatitis B virus infection combined with alcoholic fatty liver, which is used for simulating, researching and preventing and treating human chronic hepatitis B virus infection combined with alcoholic fatty liver. Background technique [0002] Hepatitis B virus (HBV) infection is a worldwide public health problem that seriously endangers human health. It can cause a variety of liver damage, including acute and chronic viral hepatitis, and is even closely related to the occurrence of liver cirrhosis and hepatocellular carcinoma. . About 2 billion people in the world have been proved to have been infected with HBV, and there are at least 350 million patients with chronic HBV infection worldwide. my country is a high-incidence area of ​​hepatitis B virus infection. About 150 million people in the country are HBV carriers, of which 20 million are chronic hepatitis B patients, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/63A01K67/027A61K35/76
Inventor 宁琴罗小平王洪武吴婷王亚琦王晓晶习东严伟明陈培哲黄丽华
Owner TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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