Tissue repair membrane with fiber structure, preparation method, using method and application thereof
A tissue repair and fiber structure technology, applied in fiber processing, textile and papermaking, spinning solution preparation, etc., can solve the problems of reduced fiber membrane porosity, low suture strength, affecting cell growth, etc., and achieve tensile strength. And the elongation at break is improved, the material source is sufficient, and the storage and transportation are simple.
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[0053] As a preferred preparation method of the tissue repair membrane of the present invention, electrospinning technology can be used, which specifically includes the following steps:
[0054] (1) Stir and dissolve the fiber material in the corresponding solvent to obtain a uniform fiber material solution;
[0055] (2) Load the fiber material solution obtained in step (1) into an electrospinning injector, and install it in the electrospinning device;
[0056] (3) Construct a turntable receiver and place it in the position of the receiving device in the electrospinning equipment;
[0057] (4) Utilize the turntable receiver in step (3) to draw and receive the filaments produced in the electrospinning process;
[0058] (5) The tissue repair membrane obtained in step (4) is placed and shaped, and part of the residual solvent is evaporated and removed;
[0059] (6) The tissue repair membrane obtained in step (5) is soaked, washed, dried, packaged and sterilized.
[0060] In the above step (1...
Embodiment 1
[0089] (1) Preparation of electrospinning solution: stir and dissolve polylactic acid-glycolic acid copolymer (PLGA) with a molecular weight of 250,000 in trifluoroethanol to form a 9% (mass to volume ratio) electrospinning solution;
[0090] (2) Put the completely dissolved electrospinning solution into the syringe and install it in the electrospinning device; among them, the electrospinning parameters are: advancing speed 6mL / h; positive high voltage: 22kV, negative high voltage: -1kV ; The receiving distance is 19cm;
[0091] (3) The receiving device is a roller, the speed of the receiving roller is 100r / min; the electrospinning device and the receiving device are turned on, and the process is stopped after 4 hours to obtain the polylactic acid-glycolic acid copolymer (PLGA) fiber membrane received by the roller;
[0092] (4) Replace the receiving device with a turntable receiver and place it in the original position of the roller. Turn on the electrospinning device, set the adva...
Embodiment 2
[0103] (1) Preparation of electrospinning solution: Polycaprolactone (PCL) with a molecular weight of 180,000 and gelatin were mixed and dissolved in hexafluoroisopropanol at a mass ratio of 4:1 to form 15% (mass volume Than) the electrospinning solution;
[0104] (2) Put the completely dissolved electrospinning solution into the syringe and install it in the electrospinning device; among them, the electrospinning parameters are: advancing speed 4.5mL / h; positive high voltage: 24kV, negative high voltage:- 3kV; The receiving distance is 21cm;
[0105] (3) The receiving device is a roller, and the speed of the receiving roller is 100r / min; the electrostatic spinning device and the receiving device are turned on, and the processing is stopped after 3.5 hours, to obtain the polycaprolactone (PCL) and gelatin composite fiber film received by the roller;
[0106] (4) Construct a turntable receiver, the turntable can be steplessly adjusted with a speed range of 0~10000r / min, and place it ...
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