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Preparation method of scorpion and maggot protein and application of scorpion and maggot protein in preparation of medicines for resisting esophagus cancer

A protein and esophageal cancer technology, applied in the preparation of scorpion maggot protein, the application field of preparation of anti-esophageal cancer drugs, can solve the problems such as the unclear cause of esophageal cancer

Inactive Publication Date: 2015-07-01
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Western medicine believes that the cause of esophageal cancer is still unclear. It is generally believed that the occurrence of esophageal cancer is related to a series of carcinogenic factors. Many carcinogenic factors act on the host, resulting in the occurrence of esophageal cancer.

Method used

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  • Preparation method of scorpion and maggot protein and application of scorpion and maggot protein in preparation of medicines for resisting esophagus cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Put 1000g of dried scorpion maggots into the room temperature puffing device (200520108339.X), fill the puffing device with carbon dioxide gas and pressurize it to 0.1~5MPa for 10~60min, and then quickly release the pressure to normal pressure, Scorpion maggots are puffed into powder;

[0033] (2) Add the puffed and pulverized scorpion maggot powder to the expansion solvent system extraction device (201210179076.6), add 1~10Kg of liquid carbon dioxide, 100~500mL of methanol and 100~300g of NH 3 ·H 2 O, pressurize to 2~10MPa to form an expansion solvent system, keep the temperature at 10~20°C for 10~30min, dissolve the protein in the scorpion maggot powder, then reduce the pressure to normal pressure, and generate the crude scorpion maggot protein and methanol- NH 3 ·H 2 O mixture;

[0034] (3) Collect the solution containing the crude protein product of scorpion maggots, and filter it with a microporous filter with a pore size of 0.5-50 μm to remove solid impuri...

Embodiment 2

[0040] (1) Put 1000g of dried scorpion maggots into the room temperature puffing device (200520108339.X), fill the puffing device with carbon dioxide gas and pressurize it to 0.1~5MPa for 10~60min, and then quickly release the pressure to normal pressure, Scorpion maggots are puffed into powder;

[0041] (2) Add the puffed and crushed scorpion maggot powder to the expansion solvent system extraction device (201210179076.6), add 5Kg of liquid carbon dioxide, 300mL of methanol and 200g of NH 3 ·H 2 O, pressurize to 5MPa to form an expansion solvent system, keep the temperature at 15°C for 20 minutes, the protein in the scorpion maggot powder is dissolved, and then reduce the pressure to normal pressure to generate the crude scorpion maggot protein and methanol-NH 3 ·H 2 O mixture;

[0042] (3) Collect the solution containing the crude protein of scorpion maggots, and filter it with a microporous filter with a pore size of 30 μm to remove solid impurities;

[0043] (4) The so...

Embodiment 3

[0048] (1) Put 1000g of dried scorpion maggots into the room temperature puffing device (200520108339.X), fill the puffing device with carbon dioxide gas and pressurize it to 0.1~5MPa for 10~60min, and then quickly release the pressure to normal pressure, Scorpion maggots are puffed into powder;

[0049] (2) Add the puffed and crushed scorpion maggot powder to the expansion solvent system extraction device (201210179076.6), add 1~10Kg of liquid carbon dioxide, 100~500mL of ethanol and 100~300g of ammonium chloride, pressurize to 2~10MPa, and form Swell the solvent system, keep the temperature at 10-20°C for 10-30 minutes, dissolve the protein in the scorpion maggot powder, and then reduce the pressure to normal pressure to generate a mixture of crude scorpion maggot protein and ethanol-ammonium chloride;

[0050] (3) Collect the solution containing the crude protein product of scorpion maggots, and filter it with a microporous filter with a pore size of 0.5-50 μm to remove sol...

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Abstract

The invention discloses a preparation method of scorpion and maggot protein and application of the scorpion and maggot protein in preparation of medicines for resisting an esophagus cancer. The preparation method of the scorpion and maggot protein comprises the following steps: puling and crushing scorpions and maggots in a room-temperature puffing device; adding to an expanded solvent system extraction device, and extracting a crude product of the scorpion and maggot protein; collecting a solution containing the crude product of the scorpion and maggot protein and filtering impurities with a micro-pore filter; carrying out ultrafiltration treatment with an ultrafilter, and collecting an ultrafiltration solution of the scorpion and maggot protein; separating the ultrafiltration solution in acetonitrile with a solid-phase extraction column, and collecting an eluting component; dissolving the eluting component into a solvent system, and carrying out liquid-phase preparation and chromatographic separation and purification with an ultrasonic standing wave; and preparing freeze-dried protein powder with a vacuum freeze drier, wherein the freeze-dried protein powder can be used for preparing medicines for resisting the esophagus cancer. The purified scorpion and maggot protein separated by the technical scheme is non-uniform protein; the relative molecular weight is 10.6-11.8KPa; the isoelectric point is pH8.9-9.6; the scorpion and maggot protein does not contain glycosyl; and the content of the purified scorpion and maggot protein is 98.2%.

Description

technical field [0001] The invention relates to a preparation method of medicine and its new application, in particular to a preparation method of scorpion maggot protein and its application in preparation of anti-esophagus cancer medicine. Background technique [0002] Esophageal cancer, also known as esophageal cancer, is a malignant tumor that occurs at the junction of the esophageal mucosa. Traditional Chinese medicine believes that the pathological mechanism of this disease is due to violent anger and worry, which injures the liver and spleen. Injury to the spleen leads to stagnation of qi, stagnation of qi leads to the inability of body fluid to flow, resulting in phlegm, stagnation of qi to the liver, and stagnation of qi to the flow of blood , Then it becomes blood stasis, blood stasis and phlegm block the esophagus, gather in the stomach and internal organs, so it is difficult for food to go down. [0003] Western medicine believes that the cause of esophageal canc...

Claims

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Application Information

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IPC IPC(8): A61K38/00A61K35/646A61P35/00C07K1/36C07K1/34C07K1/16
Inventor 徐华民赵荣华
Owner HARBIN INST OF TECH