High performance liquid chromatography determination method for identifying starch syrup adulteration in honey

A technology of high performance liquid chromatography and starch syrup, which is applied in the field of qualitative determination of starch syrup adulteration in honey by using high performance liquid chromatography, and achieves the effect of simple sample pretreatment

Inactive Publication Date: 2015-07-01
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to find a method for detecting the adulteration of starch syrup in honey that is simple to operate, easy to popularize, high in cost and can better meet the needs of actual work in view of the current situation of frequent adulteration of starch syrup in honey to meet the requirements of honey adulteration. False starch syrup identification and quality control needs

Method used

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  • High performance liquid chromatography determination method for identifying starch syrup adulteration in honey
  • High performance liquid chromatography determination method for identifying starch syrup adulteration in honey
  • High performance liquid chromatography determination method for identifying starch syrup adulteration in honey

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Experimental program
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Effect test

Embodiment 1

[0030] 1. Preparation of maltooligosaccharide standard mixture: Weigh 10.0 mg each of chromatographically pure maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, and maltoheptaose, respectively, and dissolve them in distilled water to 10 mL. Prepare a stock solution with a concentration of 1.0 mg / mL in a brown bottle.

[0031] details as follows:

[0032] Table 1 Preparation of maltooligosaccharide standard mixture

[0033]

[0034] 2. Sample pretreatment method: weigh 1g of sample, accurate to 0.001g. Dissolve in 4mL of distilled water, transfer the sample solution to OASIS HLB (6mL, 200mg) small column, and after the sample liquid flows out completely, then use 6mL of 30% acetonitrile aqueous solution to elute, collect the eluate, and wash the eluate with 0.22 μm membrane filtration to be determined. to be tested.

[0035] 3. Liquid chromatography conditions:

[0036] a) Chromatographic column: Benson BP-200Ag+ (7.8mm I.D×300mm);

[0037] b) Column t...

Embodiment 2

[0044] 1. Preparation of maltooligosaccharide standard mixture: Weigh 10.0 mg each of chromatographically pure maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, and maltoheptaose, respectively, and dissolve them in water to 10 mL. Make a stock solution with a concentration of 1.0 mg / mL in a brown bottle.

[0045] details as follows:

[0046] Table 1 Preparation of maltooligosaccharide standard mixture

[0047]

[0048] 2. Sample pretreatment method: weigh 1g of sample, accurate to 0.001g. Dissolved in 2mL of water, the resulting solution was filtered through a 0.45μm membrane filter, and tested.

[0049] 3. Liquid chromatography conditions:

[0050] a) Chromatographic column: Mitsubishi MCI GEL CK04SS (10mm I.D×200mm);

[0051] b) Column temperature: 100°C;

[0052] c) mobile phase: distilled water;

[0053] d) Flow rate: 1mL / min;

[0054] e) Injection volume: 10uL;

[0055] f) Detector: differential detector (RI);

[0056] g) Data collection time:...

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Abstract

The invention discloses a high performance liquid chromatography determination method for identifying starch syrup adulteration in honey. The invention aims to find out the determination method for starch syrup adulteration in the honey aiming at a starch syrup adulteration situation in the honey at present, wherein the determination method is simple to operate, easy to popularize and low in cost. Through pretreatment and high performance liquid chromatography detection on a sample, honey mixed with starch syrup has characteristic oligosaccharide 'fingerprint regions' (DP6-13) and a typical 'fingerprint peak' M between maltohexaose and maltoheptaose, and the fingerprint regions and the fingerprint peak are used as indices for determining that the starch syrup is mixed into the honey. The lowest detection limit of the starch syrup adulteration is 1%, and the method is applicable to identification of the starch syrup adulteration of all varieties of honey.

Description

technical field [0001] The invention relates to a method for detecting adulteration of starch syrup in honey, in particular to a qualitative determination method for identifying adulteration of starch syrup in honey by using high performance liquid chromatography. It belongs to the scope of food adulteration identification and food quality control. Background technique [0002] As a natural health food, honey has always been favored by consumers. Among the honey adulteration methods commonly used in recent years, the use of starch syrup adulteration is the most common form. At present, the main inspection methods for honey adulteration commonly used at home and abroad lack the means to identify the adulteration of starch syrup. There are some methods to solve this problem to varying degrees, but there are certain defects and deficiencies: pollen microscopy is affected by pollen There are many external influence factors in thin layer chromatography (TLC), and the error is r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/02
Inventor 熊晓辉汪丹丹陆利霞吴震威李壹丁安帮
Owner NANJING TECH UNIV
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