Micro-ecologic organic fertilizer fermented from viscera of fish and shellfish and manufacturing method thereof
A production method and technology of fish and shellfish, applied in the field of organic fertilizer production, micro-ecological organic fertilizer and its production, can solve problems that have not been seen yet, achieve the effects of rich fertility, reducing the use of pesticides, and enhancing soil fertility
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Embodiment 1
[0034] A method for making micro-ecological organic fertilizers fermented by viscera of fish and shellfish, comprising the steps of:
[0035] (1) High-density cultivation of Bacillus natto:
[0036] Use the culture medium composed of inorganic salt, C source and N source as raw materials, and use liquid three-stage seed cultivation method to cultivate at a constant temperature at 37°C and pH 7.0. Primary cultivation: inoculate the liquid activated Bacillus natto In the 100mL Erlenmeyer flask culture medium, obtain the primary seed liquid after 48 hours, then inoculate the primary seed culture liquid into the 1000mL Erlenmeyer flask and cultivate it in a shaker, obtain the secondary seed liquid after 48 hours, and then inoculate the secondary seed liquid The seed solution was placed in a 10L capacity fermenter with a stirrer and cultivated for 24 hours to obtain three levels of seed solution. The inoculum amount of each level was 10%. During this period, good ventilation con...
Embodiment 2
[0049] A method for making micro-ecological organic fertilizers fermented by viscera of fish and shellfish, comprising the steps of:
[0050] (1) High-density cultivation of Bacillus natto:
[0051] Use the culture medium composed of inorganic salt, C source and N source as raw materials, and use liquid three-stage seed cultivation method to cultivate at a constant temperature at 37°C and pH 7.0. Primary cultivation: inoculate the liquid activated Bacillus natto In the 100mL Erlenmeyer flask culture medium, obtain the primary seed liquid after 46 hours, then inoculate the primary seed culture liquid into the 1000mL Erlenmeyer flask and cultivate in a shaker, obtain the secondary seed liquid after 46 hours, and then inoculate the secondary seed liquid The seed liquid was placed in a 10L capacity fermenter with a stirrer and cultivated for 22 hours to obtain three grades of seed liquid. The seed inoculation amount of each grade was 10%. During this period, good ventilation co...
Embodiment 3
[0064] A method for making micro-ecological organic fertilizers fermented by viscera of fish and shellfish, comprising the steps of:
[0065] (1) High-density cultivation of Bacillus natto:
[0066] Use the culture medium composed of inorganic salt, C source and N source as raw materials, and use liquid three-stage seed cultivation method to cultivate at a constant temperature at 37°C and pH 7.0. Primary cultivation: inoculate the liquid activated Bacillus natto In the 100mL Erlenmeyer flask culture medium, obtain the primary seed liquid after 50 hours, then inoculate the primary seed culture liquid into the 1000mL Erlenmeyer flask and cultivate it in a shaker, obtain the secondary seed liquid after 50 hours, and then inoculate the secondary seed liquid The seed liquid was placed in a 10L capacity fermenter with a stirrer and cultivated for 26 hours to obtain three grades of seed liquid. The seed inoculation amount of each grade was 10%. During this period, good ventilation...
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