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A kind of ultrasonic extraction method of Scrophulariaceae polysaccharide

An extraction method and ultrasonic technology, applied in the directions of drug combination, antitumor drugs, etc., can solve the problems of extraction solvent consumption, long extraction time, complicated operation, etc., and achieve the effects of shortening extraction time, strong selectivity, and improving economic benefits.

Inactive Publication Date: 2016-08-24
JISHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction of polysaccharides from Scrophulariaceae Scrophulariae is mostly carried out in single batches of cans, and the yield is low, the extraction time is long, the extraction solvent is expensive, the operation is cumbersome, and the environmental pollution is serious.

Method used

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  • A kind of ultrasonic extraction method of Scrophulariaceae polysaccharide
  • A kind of ultrasonic extraction method of Scrophulariaceae polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Processing of raw materials: Radix Scrophulariae can be wet or dry. If it is wet, it will be beaten to 25 mesh. If it is dry, it will be crushed to 25 mesh. Water and a compound enzyme preparation of 3 / 1000 of the medicinal material will be added for enzymolysis. The enzymatic hydrolysis time 9 hours, enzymatic hydrolysis temperature 45°C;

[0031] The proportioning of the compound enzyme preparation is 52% of the mass fraction of cellulase, 30% of the mass fraction of pectinase, and the rest is xylanase. The average enzyme activity of the compound enzyme preparation is 18000 units;

[0032] (2) Ultrasonic extraction: using water as the solvent, the solid-liquid ratio of scrophulariaceae to water is 1:12, ultrasonic-assisted extraction of scrophulariaceae polysaccharides, and the extract is obtained after centrifugal filtration;

[0033] During the above extraction process, the extraction tank is vacuumed and broken intermittently, and vacuum pulses are used to enha...

Embodiment 2

[0043] (1) Processing of raw materials: Radix Scrophulariae can be dry or wet. If it is wet, it will be beaten to a pulp. If it is dry, it will be crushed to 45 mesh. Add water and a compound enzyme preparation of 7 / 1000 of the medicinal material to carry out enzymatic hydrolysis. The enzymatic hydrolysis time 4.5 hours, enzymatic hydrolysis temperature 48°C;

[0044] The proportioning of the compound enzyme preparation is 67% of the mass fraction of cellulase, 18% of the mass fraction of pectinase, and the rest is xylanase. The average enzyme activity of the compound enzyme preparation is 27000 units;

[0045] (2) Ultrasonic extraction: using water as the solvent, the solid-liquid ratio of scrophulariaceae to water is 1:12, ultrasonic-assisted extraction of scrophulariaceae polysaccharides, and the extract is obtained after centrifugal filtration;

[0046] During the above extraction process, the extraction tank is vacuumed and broken intermittently, and vacuum pulses are use...

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Abstract

The invention discloses an ultrasonic extraction method of polysaccharides from Scrophulariae Scrophulariae, which comprises (1) beating or pulverizing raw medicinal materials, and then enzymatically hydrolyzing them with a compound enzyme preparation; (2) the ratio of solid to liquid of Scrophulariae Scrophulariae in water is 1:10-1:100 Ultrasonic-assisted extraction is carried out, and the extract is obtained after centrifugal filtration. During the extraction process, the extraction tank is intermittently evacuated and vacuum-broken; (3) The Scrophulariaceae polysaccharide extract is loaded onto a macroporous adsorption resin chromatography column, and the vacuum is removed by Elute with deionized water and collect the eluate; (4) After concentrating the eluate under reduced pressure, add ethanol to fully stir and mix, and let stand; (5) Freeze-dry. The invention effectively integrates multiple technologies such as enzymatic hydrolysis of compound enzyme preparation, vacuum pulsation, ultrasonic assistance, resin adsorption, etc., which significantly improves the extraction rate and purity of Scrophulariaceae polysaccharides, shortens the extraction time, and has strong market competitiveness and application prospects. Broadly, the obtained Scrophulariaceae polysaccharide has obvious inhibitory effect on cancer cells.

Description

technical field [0001] The invention relates to a method for extracting polysaccharides from Chinese medicinal material Scrophulariaceae scrophulariae by using ultrasonic waves. Background technique [0002] Plant polysaccharides are an important topic in the field of biochemistry, and have become a new research hotspot at home and abroad, among which water-soluble polysaccharides extracted from Chinese herbal medicines are particularly popular. Polysaccharides are polymers that are linked by monosaccharides and have a polymerization degree of more than 100,000. Plant polysaccharides are a component commonly found in plant cell walls and cells. At present, hundreds of polysaccharides have been isolated and extracted from nature. A large number of studies have proved that polysaccharides have unique biological activities, and are the main active substances of many Chinese herbal medicines. . According to the statistics of the China Chamber of Commerce for Import and Export ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P35/00
Inventor 张朝晖佘高照张春向士全向冬梅
Owner JISHOU UNIVERSITY