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Method for improving production performance of low temperature turf by adopting strengthened compost microbial agent

A technology of microbial inoculum and compound microbial bacteria is applied in the field of improving the production performance of low-temperature turf, which can solve the problems of little research and other problems.

Inactive Publication Date: 2015-07-15
TIANJIN NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under low temperature stress, there are few studies on the effect of enhanced low temperature resistant microbial agents on turfgrass, and there are few studies on which concentration of inoculum can make turfgrass treated under low temperature stress achieve the best growth status

Method used

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  • Method for improving production performance of low temperature turf by adopting strengthened compost microbial agent
  • Method for improving production performance of low temperature turf by adopting strengthened compost microbial agent
  • Method for improving production performance of low temperature turf by adopting strengthened compost microbial agent

Examples

Experimental program
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Effect test

Embodiment 1

[0089] The preparation method of strengthening low-temperature-resistant microbial bacterial agent:

[0090] (1) Enrichment of strains: Weigh 10g of the compost sample and place it in a sterile Erlenmeyer flask, add 100mL of sterile water and shake evenly, then take 10mL of the suspension and put it in a Erlenmeyer flask filled with 100mL of enrichment medium , shake culture at 28°C, 220r / min for 3 days, which is the mixed microbial flora;

[0091] (2) Strengthening of mixed microbial flora: Spread the mixed microbial flora with different concentration gradients on beef extract peptone medium, Gao's No. 1 medium and Martin's medium, and place them in an artificial climate incubator for 2 week, the temperature is 10°C, and the enhanced complex microbial flora is obtained; the complex microbial flora of different concentration gradients refers to (μg / ml) 10 -1 、10 -2 、10 -3、10 -4 、10 -5 and 10 -6 .

[0092] (3) Cultivate the enhanced Pseudomonas stutzeri and Bacillus subt...

Embodiment 2

[0094] Method of using enhanced compost microbial agent to improve low temperature turf production performance Law :

[0095] (1) The experimental material is perennial tall fescue which is relatively common in northern my country, and the substrate used is the prepared campus soil, which is sterilized at 121°C for 30 minutes and reserved; put sterilized soil in each culture container as the substrate, and construct The thickness of the turf substrate for carpet planting is 15-16mm, and the seeding rate is 160 g m 2 , uniform and quantitative water supply every day to maintain a good water condition in the culture medium, and the position of each culture container should be changed frequently to ensure consistent light;

[0096] (2) After the plants grow to 15 days, add 15ml of the corresponding compound microbial agent respectively. During the cultivation of lawn plants, the temperature is 12°C, the relative humidity is 35%, the light is natural light penetrating into the ro...

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Abstract

The invention discloses a method for improving the production performance of low temperature turf by adopting a strengthened compost microbial agent. The strengthened compost microbial agent is composed of strengthened Pseudomonas stutzeri, strengthened bacillus subtilis, strengthened pleosporaceae and strengthened cryptococcus according to a part weight ratio of 1:1:1:1. The strengthened low temperature resistant microbial Pseudomonas stutzeri, bacillus subtilis, pleosporaceae and cryptococcus obtained from domestic waste compost are used to prepare different concentrations of the composite microbial agent, and the composite microbial agent is inoculated to a lawn building and planting system to screen out a best concentration of the composite microbial agent in order to improve the low temperature resistance, the production performance and other comprehensive quality of turf and provide technical support for winter production and storage overwintering of the turf.

Description

technical field [0001] The invention belongs to the technical field of environmental protection and relates to improving the production performance of low-temperature turf by adopting enhanced composting microbial bacterial agents. Background technique [0002] Microbial agents, especially compound microbial agents composed of a variety of microorganisms, are biotechnology developed in recent years with broad application prospects. Microbial agents can not only repair oil-contaminated soil and degrade organic pollutants in water bodies, but also prevent and control pests and diseases, promote crop production and increase production and income. Some microbial agents can improve the stress resistance of plants. Since the 1970s, European and American Japan and other countries or regions have successfully developed some composite bacterial agents, many of which have begun large-scale production and formed serialized products. Among them, EM (Effective Microorganisms), developed...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/20A01G1/00A01G7/06C05G3/00C05F17/00C12R1/38C12R1/125C12R1/01
CPCY02W30/40
Inventor 多立安赵树兰高星星
Owner TIANJIN NORMAL UNIVERSITY
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