Preparation of chitosan-carbon quantum dot composite film modified electrode and application of chitosan-carbon quantum dot composite film modified electrode to electrochemical identification of tryptophan enantiomer

A tryptophan enantiomer and carbon quantum dot technology, which is applied in the fields of biotechnology and electrochemical research, can solve problems such as unreported, and achieve the improvement of identification efficiency, environmental protection and pollution-free preparation process, and simple and easy preparation method. Effect

Active Publication Date: 2015-07-22
CHANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, carbon quantum dots have been mostly used in photovoltaic devices, cell imaging, drug release and other fields, but there are no reports on chiral sensors using carbon quantum dots as electrochemically modified materials.

Method used

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  • Preparation of chitosan-carbon quantum dot composite film modified electrode and application of chitosan-carbon quantum dot composite film modified electrode to electrochemical identification of tryptophan enantiomer
  • Preparation of chitosan-carbon quantum dot composite film modified electrode and application of chitosan-carbon quantum dot composite film modified electrode to electrochemical identification of tryptophan enantiomer
  • Preparation of chitosan-carbon quantum dot composite film modified electrode and application of chitosan-carbon quantum dot composite film modified electrode to electrochemical identification of tryptophan enantiomer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation of chitosan-carbon quantum dot composite film modified electrode includes the following steps:

[0028] (1) Weigh 2.0 g of citric acid and grind it into a white powder, put it into a 10 mL crucible, and heat and react at 200° C. for 0.5 h with a program-controlled box-type electric furnace. After the reaction, take out the solid sample, add 10mL ultrapure water, and dissolve it by ultrasonic. After the product was fully dissolved, the yellow transparent solution was obtained by suction filtration, and stored in the dark at room temperature.

[0029] (2) A three-electrode system was used in the experiment, a glassy carbon electrode (3 mm in diameter) was used as the working electrode, a platinum sheet was used as the auxiliary electrode, and a saturated calomel electrode (SCE) was used as the reference electrode. The glassy carbon electrode is the working electrode, respectively immersed in the carbon quantum dot solution prepared in step (1), and within the...

Embodiment 2

[0032] The preparation of the chitosan-carbon quantum dot composite film modified electrode and its application to the electrochemical method for identifying tryptophan enantiomers are the same as in Example 1.

[0033] The optimal recognition temperature of tryptophan enantiomers by the chitosan-carbon quantum dot composite membrane modified electrode was investigated. The prepared chitosan-carbon quantum dot composite film modified electrode was immersed in the L / D-tryptophan enantiomer solution at a concentration of 0.5mM, the standing time was 60s, and the experimental temperature was 5-35°C. The recognition effect diagram of tryptophan enantiomers is shown in Figure 4 , it can be seen that the best recognition temperature of chitosan-carbon quantum dot composite film modified electrode for tryptophan enantiomer is 15 ℃.

Embodiment 3

[0035] The preparation of the chitosan-carbon quantum dot composite film modified electrode and its application to the electrochemical method for identifying tryptophan enantiomers are the same as in Example 1.

[0036] The optimal pH for recognition of tryptophan enantiomers by the chitosan-carbon quantum dot composite membrane modified electrode was investigated. The prepared chitosan-carbon quantum dot composite film modified electrode was immersed in L / D-tryptophan solution at a concentration of 0.5mM, the standing time was 60s, and the experimental pH was 4-10. The effect diagram of enantiomer recognition is shown in Figure 5 , it can be seen that the chitosan-carbon quantum dot composite film modified electrode has the best recognition pH of tryptophan enantiomer at 7.

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Abstract

The invention relates to preparation of a chitosan-carbon quantum dot composite film modified electrode and an application of the chitosan-carbon quantum dot composite film modified electrode to electrochemical identification of a tryptophan enantiomer. A method for identifying the tryptophan enantiomer comprises the following steps: preparing carbon quantum dots, preparing the chitosan-carbon quantum dot composite film modified electrode, and identifying the tryptophan enantiomer with an electrochemical method. The preparation and the application have the benefits that a preparation method of the chitosan-carbon quantum dot composite film modified electrode is simple, convenient and feasible, a preparation process is environment-friendly and pollution-free, and the identification efficiency of the composite film modified electrode to the tryptophan enantiomer is greatly improved in comparison with that of a chitosan modified electrode to the tryptophan enantiomer.

Description

technical field [0001] The invention relates to the preparation of a chitosan-carbon quantum dot composite film modified electrode and its application in the electrochemical method to identify tryptophan enantiomers, belonging to the fields of biotechnology and electrochemical research. technical background [0002] Amino acids are the basic units of biologically functional macromolecular proteins, and proteins are the main bearers of life activities. Therefore, the separation and analysis of amino acid enantiomers plays a very important role in life sciences, medicinal chemistry, and human health. The molecular structures of enantiomers in different configurations are mirror images of each other but cannot overlap. They have the same physical properties but exhibit completely different pharmacological activities, metabolic processes and toxicological effects in vivo. As one of the essential amino acids in humans and animals, L-tryptophan cannot be synthesized by itself, bu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 孔泳欧洁陶永新戴江英
Owner CHANGZHOU UNIV
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