Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Culture method for akebiaquinata decne seedlings

A cultivation method and seedling technology, applied in the field of plant cell engineering, can solve the problems of unreported and public application, and achieve the effects of short cultivation period, large biological output and high proliferation rate

Inactive Publication Date: 2015-07-29
JIUJIANG UNIVERSITY +1
View PDF4 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The cultivation and rapid propagation of Akebia seedlings by means of plant tissue culture is a better method for obtaining Akebia seedlings on a large scale, and this method has not been reported or publicly applied

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method of using branches as explants and comprehensively processing plant tissue culture methods for rapid propagation of A. chinensis to obtain a large number of seedlings. The specific steps are as follows:

[0025] (1) Pretreatment: The pretreatment method is to immerse the stems in an aqueous solution containing 4 g / L PVP+1.5g / L Vc immediately after they are separated for 4 hours, and then place them in a moisturizing refrigeration at 4°C for 9 d ;

[0026] (2) Obtaining aseptic explants: the pretreated leaves are sterilized with 75% alcohol for 30 seconds, then sterilized with mercury for 10 minutes, and then rinsed with sterile water for use;

[0027] (3) Sprout tissue cultivation: inoculate the sterile explants obtained in step (2) into the liquid superficial sprout tissue culture medium to induce sprouts; the medium formula for sprout tissue cultivation is Ms +0.5mg / L GA3+0.1g / L PVP+30 g / L sucrose, pH 5.6; the culture conditions are: light 4h / d, temperature 25±1°C; ...

Embodiment 2

[0032] A method of using branches as explants and comprehensively processing plant tissue culture methods for rapid propagation of A. chinensis to obtain a large number of seedlings. The specific steps are as follows:

[0033] (1) Pretreatment: The pretreatment method is to immerse the branches in an aqueous solution containing 3 g / L PVP+1 g / L Vc immediately after being separated for 4 hours, and then place them in a moisturizing and refrigerating storage at 4°C for 7 days;

[0034] (2) Obtaining aseptic explants: the pretreated branches are sterilized with 75% alcohol for 30 seconds and then sterilized with mercury for 5 minutes, and then rinsed with sterile water for use;

[0035] (3) Sprout tissue cultivation: inoculate the sterile explants obtained in step (2) into the liquid superficial sprout tissue culture medium to induce sprouts; the medium formula for sprout tissue cultivation is Ms +2.0mg / L GA3+0.8g / L PVP+30 g / L sucrose, pH 5.8; culture conditions: light 5h / d, temperature ...

Embodiment 3

[0040] A method of using branches as explants and comprehensively processing plant tissue culture methods for rapid propagation of A. chinensis to obtain a large number of seedlings. The specific steps are as follows:

[0041] (1) Pretreatment: The pretreatment method is to immediately place the branches in an aqueous solution containing 3 g / L PVP+1 g / L Vc for 3 hours after being separated, and then place them in a moisturizing and refrigerating storage at 4°C for 5 days.

[0042] (2) Obtaining aseptic explants: The pretreated branches are sterilized with 75% alcohol for 30 seconds and then sterilized with mercury for 5 minutes, and then rinsed with sterile water for use.

[0043] (3) Sprout tissue cultivation: inoculate the sterile explants obtained in step (2) into the liquid superficial sprout tissue culture medium to induce sprouts; the medium formula for sprout tissue cultivation is Ms +3.0mg / L GA3+1.2g / L PVP+30 g / L sucrose, pH 5.8; culture conditions: light 5h / d, temperature 25...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Thicknessaaaaaaaaaa
Thicknessaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of plant cell engineering. Culture steps of akebiaquinata decne seedlings sequentially comprise pretreatment, aseptic explant obtaining, bud tissue culture, callus induced proliferation, embryoid induced proliferation, rooting culture and transplanting. The technical scheme for producing the akebiaquinata decne seedlings, provided by the invention, has the beneficial effects of high proliferation ratio, high biological yield, short culture period, good quality of tissue culture seedlings, healthy seedlings and high cultivation survival rate. Compared with other culture modes, a culture method for culturing the akebiaquinata decne seedlings by using pretreatment, aseptic explant obtaining, bud tissue culture, callus induced proliferation, embryoid induced proliferation, rooting culture and transplanting, disclosed by the invention, has the advantages of high propagation rate, short culture period, convenience in transfer, healthy seedlings, high cultivation survival rate and low cost, and is suitable for scale culture and production of the akebiaquinata decne seedlings.

Description

technical field [0001] The invention belongs to the technical field of plant cell engineering, and in particular relates to a method for obtaining a large number of seedlings through comprehensive treatment of plant tissue culture for rapid propagation of akebia. Background technique [0002] Akebia quinata (Houtt.) Decne. is a deciduous woody vine belonging to the family Akebia (Lardizabalaceae) of the genus Akebia. Produced in the provinces and autonomous regions of the Yangtze River Basin. Born in mountain bushes, forest margins and valleys at an altitude of 300-1500 meters. Japan and North Korea have distribution. Its stems, roots and fruits are used medicinally, and have the effects of diuresis, breast enhancement, anti-inflammation, and treatment of rheumatoid arthritis and lumbago. Akebia is a twining vine plant with a flowering period from April to May. The flowers are born on shortened side branches. The flowers are slightly fragrant and have high ornamental val...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
Inventor 贾明良廖亮徐玲玲李同建方荷芳苏慧慧
Owner JIUJIANG UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com