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Application of tilia amurensis inflorescence extractive to preparation of medicine for treating insomnia

A technology for treating medicine and tilia flower, applied in the field of medicine, can solve the problems such as the extraction process of the hypnotic active effective part of tilia flower that is not disclosed in the literature

Active Publication Date: 2015-07-29
HEILONGJIANG UNIV OF CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Up to now, there are no relevant reports on the hypnotic activity of Tilia japonica extract at home and abroad, and there is no literature disclosing the effective parts related to the hypnotic activity of Tilia japonica and its extraction process

Method used

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  • Application of tilia amurensis inflorescence extractive to preparation of medicine for treating insomnia
  • Application of tilia amurensis inflorescence extractive to preparation of medicine for treating insomnia
  • Application of tilia amurensis inflorescence extractive to preparation of medicine for treating insomnia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of embodiment 1 Tilia flower crude product extract

[0033] 1) Take 5 kg of dried Tilia japonica, add concentration according to the ratio of 1:10 to the ratio of solid to liquid and soak in 70% ethanol for 12 hours,

[0034] Reflux extraction at 80°C, the number of reflux extractions is 3 times, the time of each reflux extraction is 2 hours, and the extracts are combined;

[0035] 2) Filter the extract obtained in step 1), recover the ethanol solvent to obtain the extract, dry the extract, and pulverize it into powder to obtain 1 kg of powder;

[0036] 3) suspending the powder obtained in step 2) with water, adding ethyl acetate for liquid-liquid extraction to obtain ethyl acetate extract;

[0037] 4) Recover ethyl acetate from the ethyl acetate extract obtained in step 3) to obtain 110 g of Tilia japonica crude extract with anti-insomnia effect.

Embodiment 2

[0038] The preparation of embodiment 2 Tilia flower crude product extract

[0039] 1) Take 5 kg of dried Tilia japonica, add concentration according to the ratio of material to liquid 1:15 and soak in 70% ethanol for 16 hours,

[0040] Reflux extraction at 82°C, the number of reflux extractions is 2 times, the time of each reflux extraction is 2.5 hours, and the extracts are combined;

[0041]2) Filter the extract obtained in step 1), recover the ethanol solvent to obtain the extract, dry the extract, and pulverize it into powder to obtain 0.97kg of powder;

[0042] 3) suspending the powder obtained in step 2) with water, adding ethyl acetate for liquid-liquid extraction to obtain ethyl acetate extract;

[0043] 4) Recover ethyl acetate from the ethyl acetate extract obtained in step 3) to obtain 89 g of Tilia japonica crude extract with anti-insomnia effect.

Embodiment 3

[0044] The preparation of embodiment 3 Tilia flower crude product extract

[0045] 1) Take 5 kg of dried Tilia japonica, add concentration according to the ratio of 1:8 of solid to liquid and soak in 70% ethanol for 6 hours,

[0046] Reflux extraction at 81°C, the number of reflux extractions is 3 times, the time of each reflux extraction is 1.5 hours, and the extracts are combined;

[0047] 2) Filter the extract obtained in step 1), recover the ethanol solvent to obtain the extract, dry the extract, and pulverize it into powder to obtain 1.07kg of powder;

[0048] 3) suspending the powder obtained in step 2) with water, adding ethyl acetate for liquid-liquid extraction to obtain ethyl acetate extract;

[0049] 4) Recover ethyl acetate from the ethyl acetate extract obtained in step 3) to obtain 93 g of Tilia japonica crude extract with anti-insomnia effect.

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Abstract

The invention relates to the field of medicine, in particular to application of a tilia amurensis inflorescence extractive to preparation of a medicine for treating insomnia. A preparation method of the tilia amurensis inflorescence extractive comprises the following steps: extracting tilia amurensis inflorescence by using 70% ethyl alcohol, then extracting the tilia amurensis inflorescence by using ethyl acetate to prepare a crude extractive, carrying out gradient elution on the crude extractive through a dichloromethane-methyl alcohol mixed solution by using a silica gel column chromatography, collecting eluant prepared by the dichloromethane-methyl alcohol mixed solution with dichloromethane and methyl alcohol in a volume ratio of 15 to 1, and recycling a solvent to prepare a purified extractive, wherein the purified extractive of the tilia amurensis inflorescence is separated by an ODS column chromatography and then is eluted by a 50% methanol aqueous solution to obtain two monomeric compounds; by virtue of NMR identification, the two monomeric compounds are tiliroside and astragalin; the tiliroside is an active component; the purified extracted is analyzed by HPLC; the mass content of the tiliroside is 31.7%. Animal experiments show that the tilia amurensis inflorescence extractive is obvious in resistance to the insomnia; the dose-effect relationship research shows that the tilia amurensis inflorescence extractive is free of obvious dose dependence and low in addiction possibility; the safety of the tilia amurensis inflorescence extractive is superior to that of commonly-available chemical medicines at present.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the application of Tilia flower extract in the preparation of drugs for treating insomnia. Background technique [0002] Insomnia refers to the inability to fall asleep or maintain a sleep state, resulting in insufficient sleep, also known as sleep onset and sleep maintenance disorders. Inferior phenomenon is a common disease. [0003] According to the results of the global sleep survey in 2002, the incidence rate of insomnia in my country is as high as 45.4%. Insomnia is one of the common sufferings of people in today's society, and it is the most common clinical symptom except pain. It is predicted that by the middle of the 21st century, the number of people with insomnia in the United States will reach 100 million. The World Health Organization survey of 25,916 patients in primary medical institutions in 15 bases in 14 countries showed that 27% of them had sleep problems...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P25/20
Inventor 马微微
Owner HEILONGJIANG UNIV OF CHINESE MEDICINE
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