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Immobilized nitrifying bacteria capable of removing ammonia nitrogen in aquarium and preparation method of immobilized nitrifying bacteria

A nitrifying bacteria and aquarium technology, applied in the field of environmental purification, can solve the problems of nitrifying bacteria taking a long time for re-adsorption and enrichment, degrading ammonia nitrogen can not play a role immediately, destroying the appearance of aquarium, etc., to achieve good treatment effect, Low cost and fast removal of ammonia nitrogen

Inactive Publication Date: 2015-07-29
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the pores of the filter material are easily blocked and affect its continued use, and it must be cleaned or replaced regularly. During the cleaning or replacement of the filter material, the nitrifying bacteria attached to the filter material are also removed together. Re-adsorption and enrichment on the filter material takes a long time
Therefore, the biggest problem with commonly used filter materials for degrading ammonia nitrogen is that they cannot function immediately after being placed in the aquarium
In addition, the placement of filter materials in the aquarium is often for the purpose of beautification, and the filter material after absorbing nitrifying bacteria will destroy the appearance of the aquarium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Dissolve 25g of acrylate monomer with a molecular weight of 5000 in 36.5g of deionized water to form an emulsion, add 30g of MLVSS 15000mg / L nitrifying bacteria concentrate to the emulsion, stir quickly to make it evenly mixed to form a stable suspension, and then add 7.5g of polyethylene glycol dimethacrylate and 1g of sodium sulfite are formed through emulsion free radical polymerization to form a large block of acrylate hydrogel for immobilizing nitrifying bacteria, and this hydrogel is cut into cubes with a side length of 2mm to obtain immobilized nitrifying bacteria. Place the immobilized nitrifying bacteria obtained in the filter tank in the aquarium, and pass through the immobilized nitrifying bacteria continuously in the aquarium's own water circulation flow rate of 10 mL / min. Compared with the immobilized nitrifying bacteria without placing the immobilized nitrifying bacteria, the ammonia nitrogen removal rate can reach 98.1 %.

Embodiment 2

[0015] Dissolve 10g of acrylate monomer with a molecular weight of 10000 in 69.5g of deionized water to form an emulsion, add 15g of MLVSS 22000mg / L nitrifying bacteria concentrate to the emulsion, stir quickly to make it evenly mixed to form a stable suspension, then add 5g polyethylene glycol dimethacrylate and 0.5g sodium sulfite, through emulsion free radical polymerization, form a large block of acrylate hydrogel for immobilizing nitrifying bacteria, and cut this hydrogel into cubes with a side length of 4mm to obtain immobilized nitrifying bacteria. Place the immobilized nitrifying bacteria obtained in the filter tank in the aquarium, and pass through the immobilized nitrifying bacteria continuously in the aquarium's own water circulation flow rate of 10 mL / min. Compared with the immobilized nitrifying bacteria, the removal rate of ammonia nitrogen can reach 98.3 %.

Embodiment 3

[0017] Dissolve 20g of acrylate monomer with a molecular weight of 10000 in 46.5g of deionized water to form an emulsion, add 25g of MLVSS 18000mg / L nitrifying bacteria concentrate to the emulsion, stir quickly to make it evenly mixed to form a stable suspension, then add 7.5g polyethylene glycol dimethacrylate and 1g sodium bisulfite, through emulsion free radical polymerization to form a large block of acrylate hydrogel immobilized nitrifying bacteria, cut this hydrogel into cubes with a side length of 2mm , to obtain immobilized nitrifying bacteria. Place the immobilized nitrifying bacteria obtained in the filter tank in the aquarium, and pass through the immobilized nitrifying bacteria continuously in the aquarium's own water circulation flow rate of 10 mL / min. Compared with the immobilized nitrifying bacteria without placing the immobilized nitrifying bacteria, the ammonia nitrogen removal rate can reach 97.1 %.

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PUM

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Abstract

The invention discloses immobilized nitrifying bacteria for removing ammonia nitrogen in an aquarium and a preparation method of the immobilized nitrifying bacteria. The immobilized nitrifying bacteria comprises polymeric hydrogel with a grid structure and activated nitrifying bacteria fixed in the grids. The polymeric hydrogel is formed through free radical polymerization of water-soluble acrylate monomers. The invention further discloses a using method of the immobilized nitrifying bacteria in the aquarium, and further provides a using position of immobilized nitrifying bacteria in the aquarium. According to the immobilized nitrifying bacteria for removing ammonia nitrogen in the aquarium, provided by the embodiment of the invention, the ammonia nitrogen removal rate reaches 95%. Ammonia nitrogen in the aquarium can be treated by using nitrifying bacteria particles, high simpleness and convenience and high easiness in implementation are achieved, and the ammonia nitrogen removal rate is high.

Description

technical field [0001] The invention relates to a nitrifying bacteria in the field of environmental purification and a preparation method thereof, in particular to an immobilized nitrifying bacteria and a method for removing ammonia nitrogen in an aquarium. Background technique [0002] During the process of breeding aquatic animals in the aquarium, the feces and residual bait of aquatic animals contain a large amount of nitrogen. The nitrogen in the water mainly exists in the form of ammonia nitrogen, nitrite, nitrate and organic nitrogen. The accumulation of ammonia nitrogen and nitrite in the water body will cause a sharp drop in dissolved oxygen in the water body, an increase in harmful gases, and a large number of harmful bacteria and opportunistic pathogens, resulting in outbreaks of aquatic animal diseases and a large number of deaths. Usually, ammonia nitrogen and nitrous acid in aquarium breeding wastewater are removed by filter purification materials, such as f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34A01K63/04
Inventor 何丹农董亚梅童琴严鹏
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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