A method for degrading various phthalates by microbacteria
A technology of microbacteria and bacterial strains, which is applied in the field of biological treatment of environmental pollutants, and achieves the effects of wide application prospects, wide distribution and strong adaptability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0034] Example 1 Isolation and Identification of Bacterial Strains
[0035] Collect the activated sludge from the sewage treatment plant, weigh 5 g of the activated sludge sample into a 150 mL Erlenmeyer flask containing 50 mL of sterile water, and cultivate it at 30°C and 140 rpm for 3 days, then take 5 mL of the sludge suspension and add 100 mL PAEs (containing 50 mg / L DMP, DEP, DBP, DOP and DEHP respectively) in the above MSM medium. After culturing at 30°C and 140 rpm for 7 days, 1 mL inoculum was continuously enriched and transferred 10 times each time, and the content of PAEs in the medium was correspondingly increased to 800 mg / L. Then the 10 times acclimatized culture solution was diluted 10 3 ~10 5Spread on LB solid plates and incubate upside down at 30°C for 1-3 days. After a single colony grows on the plate, pick a single colony and streak it several times for purification, and obtain a strain of bacteria, numbered J-1. Then the strains were inoculated on LB sol...
Embodiment 2
[0039] Example 2 Degradation effect experiment of bacterial strain J-1 on various PAEs
[0040] S1. Preparation of bacterial suspension: Inoculate the purified strain J-1 into 10 mL of LB liquid medium for overnight activation to the logarithmic phase, collect the bacterial cells by centrifugation at 5000 rpm for 10 min, and wash the bacteria 3 times with PBS After resuspension, adjust OD 600 nm =1.0 as the strain suspension.
[0041] S2. Inoculate 1 mL of the above-mentioned bacterial suspension into 100 mL of MSM culture medium containing 200 mg / L concentration of PAEs (respectively containing 200 mg / L of DMP, DEP, DBP, DOP and DEHP), and use no inoculation as a control, and The pH was adjusted to 7.0, and each group was repeated three times. Incubate in a constant temperature shaker at 30°C and 150 rpm for 7 days, take samples and extract samples at 0, 1, 3, 5, and 7 days respectively, and measure the degradation of DMP, DEP, DBP, DOP and DEHP in the samples by GC / MS . ...
Embodiment 3
[0046] Example 3 Remediation Effects of Strain J-1 on Various PAEs Contaminated Soils
[0047] S1. Preparation of test soil: The soil is paddy soil from the farm of South China Agricultural University. After air-drying, it passes through a 60-mesh sieve with a pH of 6.7. Five kinds of PAEs (including DMP, DEP, DBP, DOP and DEHP) are added to it to make each The PAE content reached 100mg / kg respectively, and double-distilled water was added to adjust to the field water holding capacity (about 30%), and aged for 15 days in the dark.
[0048] S2. Experimental treatment: Weigh the above-mentioned 100 mg kg -1 Put 200 g of DBP soil in a Erlenmeyer flask, add 50 mL of bacterial suspension to the soil, and mix well. In addition, the treatment without bacteria was used as the control group. Adjust the soil to the field water holding capacity (about 30% water content), culture in a 30°C incubator in the dark, take samples and extract regularly at 0, 2, 4, 6, 8, and 10 days, and then ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More