Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Methods of controlling fungal pathogens using polyene fungicides

A fungicide, nystatin technology, applied in the field of treatment and/or prevention of sudden death syndrome, which can solve the problem of ineffectiveness of fungicides

Inactive Publication Date: 2015-08-12
BAYER CROPSCI LP
View PDF24 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In particular, to date, fungicides have been found to be ineffective

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods of controlling fungal pathogens using polyene fungicides
  • Methods of controlling fungal pathogens using polyene fungicides
  • Methods of controlling fungal pathogens using polyene fungicides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Example 1: In the agar diffusion test, the bacteriostatic zone of different concentrations of natamycin against the North American soybean sudden death syndrome pathogen was evaluated.

[0088] The PDA plate containing the two-week-old SDS pathogen was submerged in sterile distilled water and scraped off with an L-rod to release the spores. The spore solution was injected into a 50 mL conical tube through about 4 layers of gauze. Spores were quantified using a hemocytometer and then diluted to 1 x 105 Spores / mL. (Tile 1×10 on the plate 4 spores). To prepare a lawn plate of Sudden Death Syndrome of North American Soybean Death Syndrome, 100 μL of the spore suspension was spread on a commercially available PDA plate. Use a straw-plunger to punch holes in the lawn plate of SDS.

[0089] Natamycin stock solutions were diluted with sterile distilled water to concentrations of 500 ppm, 250 ppm, 100 ppm, 50 ppm, 25 ppm, 12.5 ppm, 6.25 ppm, 3.13 ppm, 1.56 ppm, 0.78 ppm and ...

Embodiment 2

[0091] Example 2: In planta (in planta) test was used to evaluate the control of natamycin on soybean sudden death syndrome.

[0092] Sorghum was prepared for inoculation. 1 to 2 liters of sorghum seeds were placed in a production bag, autoclaved, and then inoculated with 30-45 mL of SDA spore suspension. Place the bag in a fume hood at room temperature, then shake and mix every few days for 3 weeks until complete. Milo inoculum was evaluated by counting the number of spores per gram of seed using a hemocytometer. Milo grows to 4.57×10 5 Spores / g.

[0093] Enumeration of spores of the pathogen Sudden Death Syndrome of North American Soybean before soil inoculation. First, 25 mL of sterile water containing 0.1% Tween 80 was added to a sterile 50 mL conical tube. Weigh the weight of the tube. Then several grains of inoculated milo from production bags were added under aseptic conditions. Weigh the tube again to get the exact mass of inoculated spores added to the tube. T...

Embodiment 3

[0099] Example 3: An experiment of using a lower concentration of natamycin on seedlings as a watering treatment for controlling SDS in plants.

[0100] Using a similar protocol as described in Example 2, two lower concentrations of natamycin (50 ppm, 25 ppm, 10 ppm, 5 ppm and 1 ppm) were evaluated for the treatment of SDS. Two trials were performed.

[0101] The natamycin solution was used as a watering treatment in an amount of 50 mL per container. Soybean seedlings were transplanted into SDS-infected soil at a ratio of 1 × 10 SDS spores 7 Spore / container. On the second day after transplantation, natamycin solution was applied to the container. Eighteen days later the soybean roots were washed.

[0102] figure 1 and figure 2 shows the results of the first experiment, image 3 and Figure 4 The results of the second experiment are shown. In particular, the data show that plants treated with natamycin at 50 ppm, 25 ppm and 10 ppm per container had significantly lowe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to the control of fungal pathogens, such as pathogens that cause sudden death syndrome, in plants by applying one or more polyene fungicides to a plant seed, soil and / or plant roots.

Description

[0001] Cross-references to related applications [0002] This patent application claims priority to US Provisional Patent Application No. 61 / 731,160, filed November 29, 2012, and US Provisional Patent Application No. 61 / 731,468, filed November 29, 2012. technical field [0003] The present invention relates to the control of fungal pathogens in plants, such as pathogens that cause sudden death syndrome, and to the treatment and / or prevention of sudden death syndrome by the application of one or more polyene fungicides. Background technique [0004] Fungicides have many uses, including crop protection and preservatives in food, feed and cosmetics. Polyene fungicides are antifungal antibiotics that have been used in these fields. It can be obtained by fermentation of Streptomyces species commonly found in soil, such as Streptomyces natalensis. The activity of polyene fungicides derives, in part, from their ability to disrupt cell membranes by forming a complex with ergoster...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/04A01N43/90A01N63/02
CPCA01N43/22A01N43/90A01N63/02A01N43/04
Inventor M·吉亚哈伯特-戈雅J·S·马戈利斯
Owner BAYER CROPSCI LP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com