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Method for extracting puerarin from residue of radix puerariae

A technology of puerarin and kudzu slag, which is applied in the production of bulk chemicals, sustainable manufacturing/processing, chemical industry, etc., can solve the problems of waste of resources, environmental pollution, low economic benefits, etc., and achieve the effect of improving the dissolution rate

Inactive Publication Date: 2015-08-19
JINGCHU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are still many imperfections in the current kudzu root processing and extraction technology, especially the utilization of kudzu is mostly limited to the extraction of kudzu powder, and the very important medicinal active ingredients flavonoids are discarded, and the most important flavonoids are flavonoids. The active ingredient puerarin is currently a scarce product on the market, and the extraction of puerarin has become one of the research hotspots. However, many current methods still have some defects. For example, many methods use a large amount of organic solvents, and some methods give up puerarin. The utilization of starch, these have all caused the waste of resources and the pollution of the environment, and economic benefit is also low at the same time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1) drying the kudzu slag obtained after extracting kudzu powder at 40°C, and ultrafinely pulverizing it to a particle diameter of 1-20 microns;

[0035] 2) Mix the crushed kudzu slag with ethanol, the ratio of solid to liquid is 1:5g / mL, add β-cyclodextrin (mass ratio of kudzu slag to β-cyclodextrin is 1:0.2), at a temperature of 80°C Ultrasonic extraction for 30 minutes; heating to reflux at 80°C, suction filtration to extract puerarin;

[0036] 3) The filtrate was rotary evaporated at 80°C to a thick paste, while ethanol was recovered, and the obtained extract was vacuum-dried at 60°C;

[0037] 4) Perform supercritical carbon dioxide extraction on the extract, the extraction temperature is 65° C., the extraction pressure is 8 MPa, and the extraction time is 100 minutes to obtain puerarin.

[0038] With reference to the puerarin standard curve, the extraction rate of the puerarin obtained in the present invention is calculated to be 1.376% and the purity is 81.60% by ...

Embodiment 2

[0040] 1) drying the kudzu slag obtained after extracting kudzu powder at 60°C, and ultrafinely pulverizing it to a particle diameter of 1-20 microns;

[0041] 2) Mix the pulverized kudzu slag with ethanol, the ratio of solid to liquid is 1:10g / mL, add β-cyclodextrin (mass ratio of kudzu slag to β-cyclodextrin is 1:0.5), at a temperature of 60°C Ultrasonic extraction for 60min; heating to reflux at 80°C, suction filtration to extract puerarin;

[0042] 3) The filtrate was rotary evaporated at 80°C to a thick paste, while ethanol was recovered, and the obtained extract was vacuum-dried at 40°C;

[0043] 4) Perform supercritical carbon dioxide extraction on the extract, the extraction temperature is 30° C., the extraction pressure is 17 MPa, and the extraction time is 60 minutes to obtain puerarin.

[0044] With reference to the puerarin standard curve, the extraction rate of the puerarin obtained in the present invention is calculated to be 1.483% and the purity is 83.2% by us...

Embodiment 3

[0046] 1) drying the kudzu slag obtained after extracting kudzu powder at 50°C, and ultrafinely pulverizing it to a particle diameter of 1-20 microns;

[0047] 2) Mix the pulverized kudzu slag with ethanol, the ratio of solid to liquid is 1:8g / mL, add β-cyclodextrin (mass ratio of kudzu slag to β-cyclodextrin is 1:0.6), at a temperature of 55°C Ultrasonic extraction for 40 minutes; heat to reflux at 80°C, and extract puerarin by suction filtration;

[0048] 3) The filtrate was rotary evaporated at 80°C to a thick paste, while ethanol was recovered, and the obtained extract was vacuum-dried at 50°C;

[0049] 4) Perform supercritical carbon dioxide extraction on the extract, the extraction temperature is 60° C., the extraction pressure is 14 MPa, and the extraction time is 70 minutes to obtain puerarin.

[0050] With reference to the puerarin standard curve, the extraction rate of the puerarin obtained in the present invention is calculated to be 1.432% and the purity is 82.2% ...

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Abstract

The invention discloses a method for extracting puerarin from residue of radix puerariae, which comprises the following steps: extracting kudzuvine root starch to obtain slag and drying, performing ultramicro crushing to obtain the particles with diameter being 1-20 micrometers; mixing the crushed residue of radix puerariae and ethanol, adding beta-cyclodextrin and performing ultrasonic extraction; performing heating reflux, performing pumping filtration for extracting puerarin; performing rotary evaporation on a filtrate to a stiff paste, simultaneously recovering ethanol, drying the obtained extract under vacuum; and performing supercritical carbon dioxide extraction on the extract to obtain the puerarin. According to the invention, beta-cyclodextrin can increase the puerarin solubility and dissolution speed from the root of kudzu vine. The method for performing supersonic wave extraction, separating and purifying puerarin has the advantages of short production period, high efficiency, simple equipment, little boiler heat supply and cooling water, saved energy consumption, reduced environment pollution, and low product cost. A used extractant (CO2) is gas which is easily removed, the obtained product has no residual toxicity, and is especially suitable for medicine and food industries.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine extraction, and in particular relates to a method for extracting puerarin from pueraria dregs. Background technique [0002] Radix puerariae is the dried root of Pueraria lobata (Willd.) Ohwi or Pueraria thomsonii Benth, a leguminous plant. Puerarin is a unique ingredient in kudzu root and is also the main active ingredient. It can inhibit platelet aggregation, lower blood sugar and other pharmacological effects. It is also a common medicinal material for preventing and treating cardiovascular and cerebrovascular diseases. It also has the effect of hangover in daily life. The reputation of ginseng, therefore, kudzu root is widely used in functional food and biomedicine. There are still many imperfections in the current kudzu root processing and extraction technology, especially the utilization of kudzu is mostly limited to the extraction of kudzu powder, and the very importan...

Claims

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Application Information

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IPC IPC(8): C07D407/04
CPCC07D407/04Y02P20/10Y02P20/54
Inventor 朱德艳王学民王劲松徐艳张婷
Owner JINGCHU UNIV OF TECH
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