Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

High-concentration chitosan oligosaccharide and production method thereof

A production method and technology of chitosan oligosaccharides, which are applied in the production field of high-concentration chitosan oligosaccharides and high-concentration chitosan oligosaccharides, can solve the problems of incomplete dissolution of substrates, large equipment investment, and low conversion rate of substrates, and achieve improved Equipment usage efficiency and the effect of reducing production costs

Inactive Publication Date: 2015-08-19
领先生物农业股份有限公司
View PDF7 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, chitosan is used as raw material in the production of chitosan oligosaccharides by biological enzymatic hydrolysis. Due to the high viscosity of chitosan, it is difficult to achieve a substrate concentration of more than 8% during enzymatic hydrolysis.
If the substrate concentration is lower than 8%, there will be problems of incomplete dissolution of the substrate and low conversion rate of the substrate
Compared with high-concentration substrates, low-concentration substrates require more equipment turnover, large equipment investment, more energy consumption for product dehydration and drying, and higher production costs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1: the production of high concentration chitosan oligosaccharide

[0073] The implementation steps of this embodiment are as follows:

[0074] A. Pretreatment

[0075] Chitosan sold under the trade name Dongxing by Weifang Dongxing Jiake Products Factory, with a deacetylation degree of 92%, is pulverized by the JB series universal pulverizer sold under the trade name Jinbao by Jiangyin Baoli Machinery Manufacturing Co., Ltd. Sieve to obtain chitosan powder with a particle size of 80 to 100 meshes; simultaneously use water to prepare chitosan enzymes sold by Leading Bio-Agriculture Co., Ltd. under the trade name Fuside to make chitosan enzyme liquids with an enzyme activity of 500U / ml;

[0076] B. The first enzymatic hydrolysis

[0077] Add water to the reaction tank, adjust its pH to 5.3 with glacial acetic acid with a concentration of 0.8% by weight under stirring, then heat the temperature of the water to 45 ° C, then add the chitosan obtained in step A ...

Embodiment 2

[0087] Embodiment 2: the production of high concentration chitosan oligosaccharide

[0088] The implementation steps of this embodiment are as follows:

[0089] A. Pretreatment

[0090] Chitosan sold under the trade name Xingyuan by Henan Xingyuan Chemical Products Co., Ltd., with a degree of deacetylation of 94%, was hammer milled using a hammer mill sold under the trade name Eight Eagles by Tai'an Daiyue District Taifeng Agricultural Machinery Factory The chitosan powder with a particle size of 80 to 100 meshes was obtained by sieving; at the same time, the chitosan enzyme sold by Xi'an Dafengshou Biotechnology Co., Ltd. under the trade name Dafengshou was prepared into a shell with an enzyme activity of 750U / ml. Glycanase solution;

[0091] B. The first enzymatic hydrolysis

[0092] Add water to the reaction tank, adjust its pH to 5.4 with glacial acetic acid with a concentration of 1.0% by weight under stirring, then heat the temperature of the water to 46 ° C, then add...

Embodiment 3

[0102] Embodiment 3: the production of high concentration chitosan oligosaccharide

[0103] The implementation steps of this embodiment are as follows:

[0104] A. Pretreatment

[0105] Chitosan sold under the trade name Dongxing by Weifang Dongxing Jiake Products Factory, with a deacetylation degree of 90%, is pulverized using a chain crusher sold under the trade name Hang Seng by Henan Hengsheng Heavy Machinery Manufacturing Co., Ltd. Particle size is 80~100 mesh chitosan powders obtained; Simultaneously, with water, the chitosan enzyme sold by Leading Bio-Agriculture Co., Ltd. under the trade name Fuside is formulated into a chitosan enzyme solution with an enzyme activity of 860U / ml;

[0106] B. The first enzymatic hydrolysis

[0107] Add water to the reaction tank, adjust its pH to 5.5 with glacial acetic acid with a concentration of 0.5% by weight under stirring, then heat to raise the temperature of its water to 48 ° C, then add the chitosan obtained in step A sugar ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle sizeaaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to high-concentration chitosan oligosaccharide and a production method thereof. The production method comprises the steps of pretreatment, first enzymolysis, second enzymolysis, third enzymolysis and after treatment. According to the production method provided by the invention, chitosanase with high enzymatic activity is adopted to ensure rapid progression of enzymolysis reaction of a high-concentration substrate (more than 10%), reduce the viscosity of the substrate, and shorten the reaction time; and the reaction efficiency and the utilization ratio of equipment are improved, the production efficiency is improved, and the enzymolysis conversion rate of chitosan oligosaccharide reaches more than 83%. The prepared chitosan oligosaccharide contains 80-85% of chitosan oligosaccharide, 5-8% of water and 0.5-0.8% of ash.

Description

【Technical field】 [0001] The invention belongs to the field of biotechnology. More specifically, the present invention relates to a production method of high-concentration chitosan oligosaccharide, and also relates to said high-concentration chitosan oligosaccharide. 【Background technique】 [0002] Due to its good water solubility, unique physiological activities such as inhibiting tumor cells, inhibiting bacteria, promoting the proliferation of bifidobacteria, moisturizing and anti-oxidation, anti-corrosion and fresh-keeping, etc., chitosan has been widely used in food, medicine, health care, agriculture and other fields. The application prospect of chitosan oligosaccharides has become a hot spot, and the corresponding research has increased investment. [0003] The methods for producing chitosan oligosaccharides include chemical, physical and enzymatic hydrolysis. Enzyme hydrolysis has become an ideal preparation method due to its mild reaction conditions, easy control, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/26
Inventor 苏海榆高小佳徐志文
Owner 领先生物农业股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com