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Drug capable of inhibiting Newcastle disease virus

A Newcastle disease virus and drug technology, applied in antiviral agents, drug combinations, allergic diseases, etc., can solve the problem of high incidence, reduce toxic side effects and related biological pollution, good biological safety, good bioaffinity effect

Inactive Publication Date: 2015-08-26
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and treatment of Newcastle disease is mainly based on prevention. Although there are many vaccines and immunization methods, the incidence of the disease is still high.

Method used

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  • Drug capable of inhibiting Newcastle disease virus
  • Drug capable of inhibiting Newcastle disease virus
  • Drug capable of inhibiting Newcastle disease virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] EID 50 Determination of the method: take 30 SPF chicken embryos, divide them into 6 groups, and inject 10 -4 、10 -5 、10 -6 、10 -7 、10 -8 、10 -9 Chicken Newcastle disease Lasota virus stock solution, after incubation at 37°C for 72 hours, allantoic fluid was collected and EID was determined 50 =10 -8.45 / 0.2ml, take 150EID 50 is the amount of virus injected.

[0026] Determination of drug safety limits: β-chitosan with molecular weights of 10491, 8588, 7099, 5933, 5743, 4371, 3624, and 3375Da was prepared into 20g / l, 10g / l, 5g / l and 1g / l with physiological saline respectively The four gradient solutions were injected into the allantoic cavity of SPF chicken embryos respectively. Within 96 hours of observation, they survived completely and continued to be cultured until hatching. The appearance of the chicken embryos was healthy and complete, without disability.

[0027] Direct inhibition group: the β-chitosan with the molecular weight of 10491, 8588, 7099, 5933,...

Embodiment 2

[0034] Preventive effect group: the β-chitosan with the molecular weight of 10491, 8588, 7099, 5933, 5743, 4371, 3624, 3375Da was prepared into two different concentrations of 1g / L and 10g / L solutions with physiological saline respectively. After the β-chitosan with molecular weight is filtered and sterilized by the solutions of different concentrations prepared in physiological saline, the numbers 1-16 are set aside for use.

[0035] Take 9-11 day-old SPF chicken embryos, use an egg canister to observe the survival of the chicken embryos and draw the air chamber to determine the position of the hole. Use iodophor to sterilize the shell of the air chamber of the chicken embryo, and then sterilize it with 75% alcohol, and inject 0.2 mL / piece of the solution of groups 1-16 after punching holes. At the same time, the blank group was injected with normal saline, the positive control was 1 g / L, 10 g / L ribavirin and astragalus polysaccharide, and the negative control was normal sali...

Embodiment 3

[0040] Therapeutic effect group: the β-chitosan with the molecular weight of 10491, 8588, 7099, 5933, 5743, 4371, 3624, 3375Da was prepared into two different concentrations of 1g / L and 10g / L solutions with physiological saline respectively, and the different After the β-chitosan with molecular weight is filtered and sterilized by the solutions of different concentrations prepared in physiological saline, the numbers 1-16 are set aside for use.

[0041] Take 9-11 day-old SPF chicken embryos, use an egg canister to observe the survival of the chicken embryos and draw the air chamber to determine the position of the hole. After adopting povidone iodine to disinfect the shell of the chicken embryo air chamber, then disinfect with 75% alcohol, and inoculate 10 days after punching. -6 Chicken Newcastle disease Lasota virus 0.2ml / bird, the experimental group and the control group consist of 21 groups, and the blank group is injected with the same amount of normal saline. After 1 da...

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Abstract

The invention specifically relates to a drug capable of inhibiting the Newcastle disease virus, belonging to the field of marine biotechnology. the drug capable of inhibiting the Newcastle disease virus is low-molecular-weight beta-chitosan. The low-molecular-weight beta-chitosan can substantially improve expression of immune factors like IL-2, TLR-7, IFN-beta and TNF-alpha so as to excite antiviral response of an immune system and improve immunity of poultry, so attack and diffusion of the Newcastle disease virus are effectively inhibited. Research on the inhibition effects of the beta-chitosan on the Newcastle disease virus provides effective bases and possible drug research and development directions for prevention and treatment of other viral diseases.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to a medicine for inhibiting Newcastle disease virus. Background technique [0002] Chitosan is second only to cellulose and the only positively charged natural polysaccharide compound. Due to its special chemical structure and charge distribution, it is endowed with excellent biological and chemical activities. Chitosan can be divided into α-chitosan, β-chitosan and γ-chitosan according to different configurations. At present, there have been many studies on α-chitosan with the highest storage capacity in nature, and some products of α-chitosan with different molecular weights have been launched, such as bandages, agricultural fertilizers, pesticides and health products. Due to the difference in spatial structure, β-chitosan with the same molecular weight shows better solubility, higher reactivity and higher affinity for reagents than α-chitosan, and has bette...

Claims

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Application Information

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IPC IPC(8): A61K31/722A61P31/12A61P37/02
Inventor 邢荣娥何晓飞秦玉坤李克成刘松于华华李鹏程
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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