Method for recycling walnut polyphenol and flavone in walnut kernel peeling wastewater through macroporous resin

A technology of macroporous resin and walnut flavonoids, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, anti-toxins, etc., can solve problems such as high cost, complicated operation, and cumbersome process methods, and achieve production cost reduction. less, the operation process is simple and convenient

Active Publication Date: 2015-08-26
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These processes are cumbersome, complicated to operate, and costly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: reclaim the method for walnut flavonoids in walnut kernel decoating waste liquid with macroporous resin, carry out as follows:

[0025] (1) Take 500 mL of walnut kernel decoating liquid, centrifuge in batches at 3000 rpm, take the supernatant, mix, and measure the flavonoid content;

[0026] (2) AB-8 macroporous resin pretreatment

[0027] Rinse the resin fully with distilled water until the effluent is no longer white and turbid, soak it in ethanol with a concentration of 95% by mass for 12 hours to make it fully swell, then wash it with distilled water until there is no alcohol smell; soak it in 5% NaOH solution for 6 hours, Then wash with distilled water until the pH is close to neutral; soak with 5% solution HCl for 6h, then wash with distilled water until the pH is close to neutral;

[0028] (3) Dilute the concentration of walnut flavonoids in the centrifuged declothing waste liquid to 0.34 mg / mL, and adjust the pH of the solution to 7;

[0029] (4)...

Embodiment 2

[0031] Embodiment 2: reclaim the method for walnut flavonoids in walnut kernel decoating waste liquid with macroporous resin, carry out as follows:

[0032] (1) Take 500 mL of walnut kernel decoating liquid, centrifuge in batches at 4000 rpm, take the supernatant, mix, and measure the flavonoid content;

[0033] (2) AB-8 macroporous resin pretreatment

[0034] Rinse the resin fully with distilled water, soak it in ethanol with a concentration of 95% by mass for 12 hours to make it fully swell, then wash it with distilled water until there is no alcohol smell; soak it with 5% NaOH solution for 6 hours, then wash it with distilled water until the pH is close to neutral property; soak in 5% solution HCl for 6h, then wash with distilled water until the pH is close to neutral;

[0035] (3) Dilute the concentration of walnut flavonoids in the centrifuged declothing waste liquid to 0.5 mg / mL, and adjust the pH of the solution to 8;

[0036] (4) On the column, the adsorption time i...

Embodiment 3

[0038] Embodiment 3: reclaim the method for walnut flavonoids in walnut kernel decoating waste liquid with macroporous resin, carry out as follows:

[0039] (1) Take 500 mL of walnut kernel decoating solution, centrifuge in batches at 5000 rpm, take the supernatant, mix, and measure the flavonoid content;

[0040] (2) AB-8 macroporous resin pretreatment

[0041] Rinse the resin fully with distilled water, soak it in ethanol with a concentration of 95% by mass for 12 hours to make it fully swell, then wash it with distilled water until there is no alcohol smell; soak it with 5% NaOH solution for 6 hours, then wash it with distilled water until the pH is close to neutral property; soak in 5% solution HCl for 6h, then wash with distilled water until the pH is close to neutral;

[0042] (3) Dilute the concentration of walnut flavonoids in the centrifuged declothing waste liquid to 0.67 mg / mL, and adjust the pH of the solution to 9;

[0043] (4) On the column, the adsorption tim...

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PUM

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Abstract

The invention discloses a method for recycling walnut polyphenol and flavone in walnut kernel peeling wastewater through macroporous resin. The method comprises the operating steps of pretreatment of the walnut kernel peeling wastewater, separation, purification and regeneration of the macroporous adsorption resin, concentration and drying. The concentration of the walnut flavone in the pretreated walnut kernel peeling wastewater is reduced to 0.34mg / mL-0.67mg / mL through dilution, the pH value is adjusted to be 7-9 through concentrated hydrochloric acid, a solution is poured into an adsorption tank filled with the macroporous resin to be adsorbed, the adsorption time is more than 4 hours, after adsorption is finished, desorption is conducted through 60%-80% ethyl alcohol to obtain an ethyl alcohol walnut flavone solution, the solution is processed through vacuum evaporation, concentration and drying, the ethyl alcohol is recycled, and finally fine walnut flavone extract is obtained. The recycling rate of the walnut flavone is more than 70%, and the purity of the walnut flavone is more than 50%. The technological process is simple, the cost is low, the benefit is high, the macroporous resin can be regenerated and recycled, and the method is suitable for industrial recycling of walnut flavone compounds in the walnut kernel peeling wastewater.

Description

technical field [0001] The invention relates to a method for recovering walnut flavonoids in walnut kernel decoating waste liquid with a macroporous resin, which belongs to the field of deep processing of food by-products, and specifically relates to a processing method for comprehensively utilizing walnut kernel decoating liquid resources to extract walnut flavonoids. Background technique [0002] There are rutin, ellagic acid, salidroside, kalanchoside, quercetin, catechin and other polyphenol flavonoids in the walnut kernel (Standan, et al. Food Industry Science and Technology, 2014, 2:375) , is a class of natural antioxidants with strong antioxidant activity, including scavenging free radicals, preventing cardiovascular diseases, preventing cancer, anti-radiation, delaying body aging and other functions, and has extremely high nutritional and health value. [0003] With the rapid development of the food industry, walnut products are constantly being introduced, among whi...

Claims

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Application Information

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IPC IPC(8): A61K36/52A61P39/06A61P35/00A61P17/16A61P25/00A23L1/29A23L33/00
Inventor 陈朝银宋浩赵声兰熊向峰韩本勇葛锋刘迪秋
Owner KUNMING UNIV OF SCI & TECH
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