Method for extracting acetylcholinesterase by use of discarded organ-chicken heads in chicken manufacturing process
A technology of acetylcholinesterase and processing, which is applied in the direction of biochemical equipment and methods, enzymes, hydrolytic enzymes, etc., can solve the problems of high price, waste of grain raw materials, and complicated preparation process, and achieve easy storage, low cost, and high realization. The effect of value
Inactive Publication Date: 2015-09-02
SOUTH CHINA UNIV OF TECH
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Abstract
The invention provides a method for extracting acetylcholinesterase by use of discarded organ-chicken heads in the chicken manufacturing process. The discarded organ-chicken brain during chicken processing is used as the material source of enzyme. The method comprises the following steps: processing of chicken brains, preparation and refrigerated centrifuge of a homogenate and vacuum freeze drying of a crude extract, and through the steps, the acetylcholinesterase product can be obtained. As the acetylcholinesterase product prepared from traditional enzyme sources is high in cost and low in yield while the imported enzyme sources are high in price and relatively long in cycle, through screening of enzyme sources, the discarded organs-chicken brains in the chicken manufacturing process are fully utilized to achieve high-value utilization and reduce the cost, and through optimization and inspection, the enzyme activity is relatively high, the enzyme activity of the crude enzyme can reach 3.0-5.0 U/mL, the specific activity can reach 0.35-0.5 U/mg, and accordingly, a foundation is laid for follow-up preparation of the pure acetylcholinesterase product.
Application Domain
Hydrolases
Technology Topic
ChemistryFreeze dry +8
Examples
- Experimental program(3)
Example Embodiment
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Example Embodiment
PUM
Property | Measurement | Unit |
Enzyme activity | 6.3178 | U/ml |
Specific vitality | 0.4254 | U/mg |
Specific vitality | 0.4836 | U/mg |
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