Application of magnesium ion to improvement of plant photosynthesis efficiency
A photosynthetic efficiency, magnesium ion technology, applied in the application, plant growth regulator, plant growth regulator and other directions, can solve the problems of crop yield affecting plant growth, decrease of leaf stomatal conductance, weakened transpiration, etc., to restore photosynthetic efficiency. and related enzyme activities, reducing negative effects and improving yield
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Embodiment 1
[0021] Example 1: The cultivation and processing of SB, RB plants, concrete steps are as follows:
[0022] 1. The experimental materials are SB and RB seedlings
[0023] After SB and RB seeds are sterilized and accelerated germination, accelerate germination in the dark at 25°C, and transfer to 0.5mmol / L CaCl when the root grows 2cm 2 Equilibrate in the solution for one day, then transfer to 1 / 4 Hoagland culture medium for one week, transfer to Hoagland culture medium after growing two leaves, and use it for this experiment when the seedling grows to four pairs of leaves;
[0024] 2. Configuration concentration 0, 50, 100, 200, 400 μmol / LAlCl 3 Treatment solution and adding 100 μmol / L MgCl 2 AlCl 3 Treatment solution; AlCl with a concentration of 0 3 No MgCl added 2 Treated plants were used as control (CK);
[0025] 3. Use the above-mentioned different concentrations of AlCl 3 Treatment solution and adding 100 μmol / L MgCl 2 AlCl 3 SB and RB seedlings were treated wi...
Embodiment 2
[0026] Example 2: Adopt the 3rd step in the embodiment 1 to process the plant after 4 days, use the Yaxin-1102 portable photosynthetic transpiration instrument of Beijing Yaxin Liyi Technology Co., Ltd. to measure the net photosynthetic rate, transpiration rate and stomatal conductance of soybean according to the instructions; Such as figure 1 shown in AlCl 3 Under the condition of treatment, the decrease of net photosynthetic rate of RB soybean was not as obvious as that of SB soybean, only with MgCl 2 In the case of treatment, the net photosynthetic rate of RB and SB both increased, and the increase of RB was more obvious than that of SB. When magnesium ions were added under a certain concentration (50, 100, 200 μmol / L) of aluminum stress, the The photosynthetic rate of the two soybeans was relatively increased when the concentration of aluminum stress was low, and the increase of SB was more obvious than that of RB. The above results show that MgCl 2 It has the effect...
Embodiment 3
[0027] Example 3: Take the SB soybean roots treated for 4 days in Example 1 as the research material, after separating and purifying the plasma membrane, use Bradford's method to measure the concentration of the plasma membrane protein, detect the purity of the plasma membrane protein and measure the plasma membrane H + - the activity of ATPase; H + - The determination of ATPase activity was carried out in a 0.5 ml reaction system; the reaction system contained 50 mM BTP / MES, 5 mM MgSO 4 , 50 mM KCl, 0.02% Brij (w / v), 50 mM KNO 3 , 1 mM (NH 4 ) 2 MoO 4 , 1 mM NaN 3 , 4 mM ATP-Na 2 , start the reaction after adding 500 μg of plasma membrane protein; place the reaction mixture in a 30°C water bath for 30 min, add 1 ml of reaction stop solution [containing 2% H 2 SO4 (v / v), 5% SDS (w / v) and 0.7% (NH 4 ) 2 MoO 4 (w / v)], immediately added 50 μl of Vc and left at room temperature for about 20 min, and measured the absorbance at a wavelength of 700 nm. Calculate the rel...
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