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A kind of double-layer composite dura mater and preparation method thereof

A dura mater and composite type technology, which is applied to the field of double-layer composite dura mater and its preparation, can solve the problems of inability to match the growth rate of the new dura mater in the degradation rate, lack of biological activity, insufficient mechanical strength, etc., and achieve proper biodegradability. properties and biological activity, improving resistance to enzymatic degradation, and improving biological stability

Active Publication Date: 2017-08-22
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) The mechanical strength of the material is not high enough to meet the requirements for medical sutures;
[0006] (2) The material itself does not have the characteristics of waterproof and sealing, resulting in cerebrospinal fluid leakage or adhesion during the use of dura mater repair materials;
[0007] (3) The material cannot be degraded in the body or the degradation time is too fast, the resistance to enzymolysis is weak, and the degradation rate cannot match the growth rate of the new dura mater;
[0008] (4) The material lacks biological activity, and the ability to promote cell growth and formation of new dura mater is weak
However, the acellular dermal matrix also has some disadvantages, such as insufficient mechanical strength, poor resistance to enzymolysis, large pores leading to cerebrospinal fluid leakage, etc.

Method used

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  • A kind of double-layer composite dura mater and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] (1) Preparation of matrix layer

[0052] Modification of medical purified pig dermis: put 100 parts by weight of medical purified pig dermis into a stainless steel drum, add 100 parts by weight of 0.01mol / L phosphate buffer at room temperature, soak for 1 hour, and then use 0.25M Na 2 CO 3 -NaHCO 3 The buffer solution adjusts the pH of the liquid in the drum to 8.5, adds 5 parts by weight of triglycidyl isocyanurate, starts the drum, reacts for 10 hours, and the reaction temperature is 30°C;

[0053] Washing: Weigh 100 parts by weight of the modified medical purified pig dermis, add it to a stainless steel drum, add 200 parts by weight of normal temperature water, wash with water for 10 minutes, discard the waste liquid, and repeat the washing process for 3 times; add 200 parts by weight of normal temperature water , washed with water for 30 minutes, discarded the waste liquid, and repeated the washing twice; added 200 parts by weight of normal temperature water, wash...

Embodiment 2

[0064] (1) Preparation of matrix layer

[0065] Modification of medical purified pig dermis: put 100 parts by weight of medical purified pig dermis into a stainless steel drum, add 300 parts by weight of 0.05mol / L phosphate buffer at room temperature, soak for 2 hours, then use 0.25M Na 2 CO 3 -NaHCO 3 The buffer solution adjusts the pH of the liquid in the drum to 9.5, adds 12 parts by weight of triglycidyl isocyanurate, starts the drum, reacts for 15 hours, and the reaction temperature is 37°C;

[0066] Washing: Weigh 100 parts by weight of the modified medical purified pig dermis, add it to a stainless steel drum, add 300 parts by weight of normal temperature water, wash with water for 20 minutes, discard the waste liquid, and repeat the washing process 5 times; add 300 parts by weight of normal temperature water , washed with water for 45 minutes, discarded the waste liquid, and repeated water washing for 4 times; added 300 parts by weight of normal temperature water, wa...

Embodiment 3

[0077] (1) Preparation of matrix layer

[0078] Modification of medical purified pig dermis: put 100 parts by weight of medical purified pig dermis into a stainless steel drum, add 500 parts by weight of 0.1mol / L phosphate buffer at room temperature, soak for 3 hours, and then use 0.25M Na 2 CO 3 -NaHCO 3 The buffer solution adjusts the pH of the liquid in the drum to 10.5, adds 20 parts by weight of triglycidyl isocyanurate, starts the drum, reacts for 20 hours, and the reaction temperature is 42°C;

[0079] Washing: Weigh 100 parts by weight of the modified medical purified pig dermis, add it to a stainless steel drum, add 400 parts by weight of normal temperature water, wash with water for 30 minutes, discard the waste liquid, and repeat the washing for 6 times; add 400 parts by weight of normal temperature water , washed with water for 60 minutes, discarded the waste liquid, and repeated the washing for 5 times; added 400 parts by weight of normal temperature water, wash...

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Abstract

The invention discloses a double-layer composite cerebral dura mater, and a preparation method thereof. According to the preparation method, medical purified pig dermis is modified with a cross-linking agent so as to obtain a matrix layer; the matrix layer is coated with a degradable high molecular membrane solution via coating method, and then is subjected to water washing and drying so as to obtain the double-layer composite cerebral dura mater. The double-layer composite cerebral dura mater repairing material is excellent in flexibility, mechanical properties, biocompatibility, and biodegradability, and is capable of preventing cerebrospinal fluid leakage and brain tissue adhesion effectively. Compared with electrostatic spinning, the coating method is capable of increasing peel strength of the matrix layer with an impervious layer; and application prospect of the double-layer composite cerebral dura mater repairing material is promising.

Description

technical field [0001] The invention relates to a double-layer composite dura mater and a preparation method thereof, belonging to the field of medical biomaterials. Background technique [0002] The dura mater is a layer of hard membranous tissue between the inside of the skull and the brain tissue. It is composed of a collagen fiber network and fibroblasts entwined in it. It is close to the inside of the skull and its main function is to protect the brain. When a person suffers from an intracranial tumor, the dura mater tissue is susceptible to erosion and damage; in addition, due to surgery, trauma and other reasons may also cause dura mater defects (Chen Zhenjun. Research and application of dura mater substitute materials [J]. Chinese tissue engineering and clinical rehabilitation , 2008, 12(14): 2697-2700.). Dural defects can cause a series of complications such as brain iatrogenic injury, cerebrospinal fluid leakage, intracranial infection, and secondary epilepsy. Ti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/24
Inventor 但卫华陈娇但年华
Owner SICHUAN UNIV
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