A Strain of Leuconostoc enterolis and Its Application in Low Temperature Silage
A technology of Leuconostoc enterococci and silage, applied in the field of microorganisms, can solve the problems of difficult to ferment high-quality feed and unfavorable growth of lactic acid bacteria
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Embodiment 1
[0040] Example 1, Isolation and Identification of Leuconostoc mesenteroides QH1-605
[0041] 1. Isolation and screening of strain QH1-605
[0042] Take 10g of reeds from Bird Island Wetland in Qinghai Lake, Qinghai Province, China, put them into 90mL of sterile water, shake for 10 seconds, draw 1mL of liquid into a 1.5mL centrifuge tube, and dilute 10 1 , 10 3 , 10 5 Take 20 μL of the diluted liquid and spread it on the MRS solid medium, culture anaerobically at 30°C for 48 hours, take a single colony MRS agar medium for expansion culture, and one of the obtained strains is numbered QH1-605.
[0043] Wherein, the solvent of the MRS solid medium is water, the solute and its concentration are as follows: peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, glucose 20g / L, sodium acetate trihydrate 5g / L, spit Temperature 80 1g / L, dipotassium hydrogen phosphate 2g / L, ammonium citrate 2g / L, magnesium sulfate 2g / L, manganese sulfate 0.25g / L, agar 17g / L.
[0044] 2. Identificati...
Embodiment 2
[0057] Embodiment 2, the growth of Leuconostoc mesenteroides QH1-605 at different temperatures, pH and salt concentrations
[0058] 1. Growth of Leuconostoc mesenteroides QH1-605 at different temperatures
[0059] Inoculate the activated Leuconostoc mesenteroides QH1-605CCTCC NO: M2015254 in MRS liquid medium, culture at 5, 10, 45 and 50°C for 24 hours, and measure the light absorption at 600nm with a spectrophotometer value (OD600) to observe the growth of Leuconostocmesenteroides QH1-605 at different temperatures.
[0060] Wherein, the solvent of MRS liquid medium is water, and the solute and its concentration are as follows: peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, glucose 20g / L, sodium acetate trihydrate 5g / L, Tween 80 1g / L, dipotassium hydrogen phosphate 2g / L, ammonium citrate 2g / L, magnesium sulfate 2g / L, manganese sulfate 0.25g / L; pH6.8.
[0061] The results are shown in Table 2. It can be seen that Leuconostoc mesenteroides QH1-605CCTCCNO:M 2015254 grow...
Embodiment 3
[0075] Embodiment 3, the antibacterial activity assay of Leuconostoc mesenteroides QH1-605
[0076] Bacteria tested: Micrococcus luteus, Micrococcus luteus, Salmonella and Escherichia coli.
[0077] 1. Inoculate the activated Leuconostoc mesenteroides QH1-605CCTCC NO: M2015254 in the MRS liquid medium (same formula as above, pH 6.8), culture at 30°C for 48 hours, centrifuge the fermentation broth at 10,000rpm for 5min, take out Serum was used for measurement.
[0078] 2. Measure its antibacterial activity by the Oxford cup double-layer plate method, heat the sterilized agar medium to completely melt, pour it into a petri dish, 15ml per dish (lower layer), and wait for it to solidify. In addition, cool the melted PDA medium to about 50°C and mix it with the bacteria to be tested, and add 5 ml of the culture medium mixed with the bacteria to the solidified medium to be solidified (upper layer). Directly place an Oxford cup (a round tube with an inner diameter of 6mm, an outer ...
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