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Application of GABA compounds in accumulating intracellular alkaloids of periwinkle callus

A technology of callus and aminobutyric acid, applied in the field of application of aminobutyric acid compounds, can solve problems such as unsatisfactory results

Active Publication Date: 2017-12-05
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Combined with optimization of culture conditions, precursor feeding or treatment with various regulatory factors to increase the content of indole alkaloids, but the effect is not very satisfactory

Method used

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  • Application of GABA compounds in accumulating intracellular alkaloids of periwinkle callus
  • Application of GABA compounds in accumulating intracellular alkaloids of periwinkle callus
  • Application of GABA compounds in accumulating intracellular alkaloids of periwinkle callus

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Experimental program
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Effect test

Embodiment 1

[0020] Compound (I-1)-compound (I-4) were respectively dissolved in deionized water to prepare 1 ppm and 10 ppm aqueous solutions.

[0021] The periwinkle callus cells are placed in a 250mL Erlenmeyer flask containing 50mL of culture solution, and the inoculation amount is 1.5-2g / bottle. The culture solution is MS culture solution containing compound (I-1)-compound (I-4) each 1mg / L (in the form of aqueous solution), 3wt% sucrose, pH6.0 (before sterilization), at 25°C, Shaker (100r·min -1 ), cultured under light for 12h. The cultured periwinkle callus cells were homogenized with 80% methanol aqueous solution, extracted overnight, the methanol was evaporated under reduced pressure, and the pH value of the aqueous phase was adjusted to 3. Then extract with petroleum ether, adjust the pH value of the water phase to 8.5, extract with dichloromethane, measure the OD value of the organic layer at 280mm, and evaporate the dichloromethane to dryness under reduced pressure to obtain t...

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Abstract

The invention discloses the application of an aminobutyric acid compound in accumulating alkaloids in periwinkle callus cells, and the alkaloids are total indole alkaloids, vinblastine or amarine; the GABA described in the invention The salicylate compound has cumulative effects on the total indole alkaloids, vinblastine and amarine in the periwinkle callus cells, and the compound provides a basis for the research and development of new drugs.

Description

(1) Technical field [0001] The invention relates to the application of aminobutyric acid compounds, in particular to the application of aminobutyric acid compounds in accumulating alkaloids in periwinkle callus cells. (2) Background technology [0002] Periwinkle is native to East Africa, and is currently grown in many areas of my country as medicinal plants and ornamental plants. The main medicinal components of periwinkle are indole alkaloids. Among them, vinblastine (vinblastine) and vincristine (vincfistine) are the most widely used natural plant anti-tumor drugs at present, and can be used to treat Hodgkin's disease, malignant lymphoma, acute leukemia, villous epithelial cell carcinoma and other diseases. Salt has been widely used clinically; in addition, ajmalicine (ajmalicine) can be used to treat diseases such as hypertension, arrhythmia; Serpentine (serpentine) can be used as analgesic; , diuretic and hypoglycemic effects, but also the synthetic precursor of vinbl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04
Inventor 刘幸海杨明艳杨二冰臧洪俊王勇李正名
Owner ZHEJIANG UNIV OF TECH