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Method for researching oryza sativa and pathogen interaction mode

A technology for pathogens and rice, applied in the field of plant biology, can solve the problem of inaccurate research results

Active Publication Date: 2015-09-16
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the shortcomings and deficiencies in the above-mentioned prior art, and provide a method for studying the interaction mode of rice and pathogens (Pyricularia oryzae), in order to overcome the inaccurate problem of the research effect of the existing single technology

Method used

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  • Method for researching oryza sativa and pathogen interaction mode
  • Method for researching oryza sativa and pathogen interaction mode
  • Method for researching oryza sativa and pathogen interaction mode

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Embodiment 1

[0031] A method based on transcriptome, proteome (iTRAQ) and miRNA group to jointly study the interaction mechanism of rice-Pyricularia oryzae

[0032] 1. Differential expression profile analysis.

[0033] 1. Sample Preparation

[0034] Rice (H4) and Zhongerruanzhan (ZE) were inoculated with blast fungus strain GD0193 at the 3-4 leaf stage, with mock inoculation as a control; rice leaves were collected 0h and 24h after inoculation, put into cryopreservation tubes, and liquid nitrogen After quick-freezing, it was stored in a -80°C ultra-low temperature refrigerator for later use; each test subject took a complete backup of the material at the same time for qRT-PCR verification.

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Abstract

The invention discloses a method for researching an oryza sativa and pathogen interaction mode, and belongs to the field of plant biotechnology. The method for researching the interaction mode of oryza sativa in response to pathogen infection through integrated application of a transtriptome, a proteome and a miRNA group comprises the concrete steps: A, respectively taking oryza sativa leaf blade total RNAs, Small RNAs and total proteins of a disease-resistant material and a susceptible material at different time points before and after pathogen inoculation; B, respectively carrying out differential-expression spectrum analysis, differential proteomics analysis and miRNA identification and target gene analysis; and C, integrating and associating differential genes, proteins and miRNA target genes identified by the three ways, to reveal disease-resistant and susceptible reaction paths activated or inhibited in the oryza sativa and pathogen interaction process, so as to relatively systematically illuminate an oryza sativa-magnaporthe oryzae interaction mechanism. The method can quickly and effectively screen candidate genes associated with oryza sativa disease resistance.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and specifically relates to a method for studying the interaction mode of rice and pathogens, more specifically, a method for jointly studying the interaction mechanism of rice and blast fungus based on transcriptome, proteome (iTRAQ) and miRNA group . Background technique [0002] Rice (Oryza Sativa) is one of the most important food crops in the world, feeding more than half of the global population. Rice blast caused by the infection of Magnaporthe oryzae is an important limiting factor in rice production. It has the characteristics of fast transmission, wide occurrence range and serious damage. With the successive completion of the genome sequencing projects of rice and Magnaporthe grisea, the research on the interaction mechanism of rice and Magnaporthe grisea has become a model system for the study of plant-pathogen interaction mechanism. [0003] The interaction between rice and Magnap...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N30/88G01N27/62
CPCG01N30/88G01N33/6848C12Q1/6816C12Q1/6869G01N2030/8831G01N2333/415C12Q2565/50C12Q2525/191
Inventor 王加峰杨瑰丽孙大元黄翠红陈志强王慧
Owner SOUTH CHINA AGRI UNIV
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