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Enterobacter hallii cl2013 and method for preparing hexavalent chromium repairing bacterial agent

A technology of Enterobacter hallii and repairing bacterial agent, which is applied in the field of preparing hexavalent chromium repairing bacterial agent, and can solve the problems of high acidity, difficult to meet the standard, and high organic matter content of pressed sewage.

Inactive Publication Date: 2018-12-18
TAIYUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, marigold press wastewater has high acidity and high organic matter content, and there are problems such as high energy consumption and difficulty in reaching the standard during the treatment process.

Method used

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  • Enterobacter hallii cl2013 and method for preparing hexavalent chromium repairing bacterial agent
  • Enterobacter hallii cl2013 and method for preparing hexavalent chromium repairing bacterial agent
  • Enterobacter hallii cl2013 and method for preparing hexavalent chromium repairing bacterial agent

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Embodiment 1

[0046] A strain of Enterobacter hormaechei (Enterobacter hormaechei) CL.2013 was deposited in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on January 27, 2015, with the preservation number CGMCC No.10451, address: Beichen, Chaoyang District, Beijing Institute of Microbiology, Chinese Academy of Sciences, No. 1 Yard, West Road.

[0047] The Enterobacter hallii CL.2013 is isolated and screened from the soil of the chromium slag yard. The strain was cultured on LB medium, the cell shape was rod-shaped, and Gram staining was negative; after 3 days of culture, the color of the colony was milky white, and the shape of the colony was round and smooth. After physiological and biochemical identification and 16S rDNA phylogenetic analysis, the new strain was identified and named Enterobacter hormaechei (Enterobacter hormaechei) CL.2013.

Embodiment 2

[0049] Utilize Enterobacter hallii CL.2013 of the present invention and marigold waste water, waste residue to prepare hexavalent chromium repair solid bacterial agent, and its method comprises:

[0050] (1) Prepare strain activation medium Add glucose 1g / L to LB medium, sterilize for 15-30min, and set aside;

[0051] (2) Preparation of activated seeds Insert the Enterobacterhormaechei CL.2013 strain of the present invention stored in inclined test tubes into the medium prepared in (1), at 28-35°C, 120-180r / min Shake culture for 12-16 hours to obtain activated seeds;

[0052] (3) Preparation of liquid seed culture solution Adjust the pH of the waste water to 6.5-7.5 with aqueous sodium hydroxide solution to extract lutein from marigolds, and sterilize at 115-121°C for 15-30 minutes;

[0053] (4) Preparation of liquid seeds Take the culture medium prepared in (3) and inoculate the activated seeds prepared in (2), the inoculum amount is 8%-10%, shake culture at 30-37℃, 120-200r...

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Abstract

A strain of Enterobacter hormaechei CL.2013 isolated and screened from the soil of the chromium slag yard was preserved on January 27, 2015, with the preservation number CGMCC No.10451. The strain was cultured on LB medium, the cell shape was rod-shaped, and Gram staining was negative; after 3 days of culture, the color of the colony was milky white, and the shape of the colony was round and smooth. The strain was confirmed by physiological and biochemical reactions and molecular biological identification Enterobacter hormaechei. Enterobacter hallii CL.2013 strain has a strong reducing effect on hexavalent chromium. The invention also discloses a method for preparing a hexavalent chromium restoration bacterial agent by using Enterobacter hallii CL.2013 strain and marigold processing waste liquid and waste residue. The method is simple, low in cost, has no secondary pollution, is convenient to use and has broad market expectation.

Description

technical field [0001] The invention relates to the field of microbes and applications, in particular to the preparation of Enterobacter hallii with reducing effect on hexavalent chromium and the fermentation liquid of the enterobacter hallii and the processing of waste liquid and waste residue by using the enterobacter halloi and marigold A method for preparing hexavalent chromium repairing bacteria agent. Background technique [0002] Chromium (Ⅵ) is the second most harmful heavy metal in groundwater, soil and sediment. It ranks 6th in the US EPA priority pollutant list. It is one of the 117 priority pollutants that are extremely harmful to the human body. It has acute, Subacute and chronic toxicity, carcinogenesis, distortion and mutagenesis. Due to the chromate ion (CrO 4 2- ) and sulfate ion (SO 4 2- ) structural similarity, chromate ions can easily enter the biomembrane through the sulfate ion channel, and further react with certain substances in the cytoplasm, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/205C12R2001/01
Inventor 李维宏岳秀萍马小丽
Owner TAIYUAN UNIV OF TECH
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