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Method for detecting volatile components in tobacco leaves

A technology for volatile components and detection methods, applied in the field of detection of volatile components in tobacco leaves, can solve problems such as no literature reports, and achieve the effects of simple and rapid sample pretreatment, high sensitivity, and avoiding the pretreatment process.

Inactive Publication Date: 2015-09-23
CHINA TOBACCO ZHEJIANG IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The use of MAE-HS-SPME for the detection of volatile components in tobacco leaves has not been reported in the literature at home and abroad

Method used

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  • Method for detecting volatile components in tobacco leaves
  • Method for detecting volatile components in tobacco leaves
  • Method for detecting volatile components in tobacco leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Weigh 0.5g of shredded tobacco sample (produced in Hunan) into a conical flask, add 20mL of distilled water, plug it with a glass stopper, and let it stand overnight. The solution was transferred to a three-necked flask and the solution was filled to 40 mL with distilled water.

[0046]Put the extraction fiber head into the extraction handle to form a manual SPME sample injector, insert it into the vaporization chamber with a temperature of 250°C, retract the fiber head after aging for 30 minutes, and pull out the sample injector.

[0047] Set the microwave power to 600W, the extraction time to 10min, use 50μm / 30μm DVB / CAR / P DMS fiber coating, insert the solid-phase microextraction head into the self-made diaphragm (the diaphragm acts as a seal to prevent the gas phase volatiles from diffusing into the air) adsorption on top of the sample.

[0048] After the extraction was completed, the solid-phase microextraction needle was inserted into the GC inlet, and analyzed at...

Embodiment 2～4

[0054] The difference with Example 1 is only: the extraction time is different, the extraction time of Examples 2~4 is successively 6min, 8min and 12min, the chromatographic total peak area of ​​Example 1~4 is as follows figure 2 As shown, after the extraction time is greater than 10min, the total peak area has a downward trend, which is due to the fact that in a short period of time, the low-volatility components have been extracted, the extraction time is too long, and the electromagnetic radiation reduces the stability of the components, resulting in Components with poor thermal stability decompose.

Embodiment 5～7

[0056] The only difference from Example 1 is that the origins of the tobacco leaf samples are different.

[0057] The total ion chromatogram of embodiment 1,5,6,7 gained is respectively as follows image 3 , Figure 4 , Figure 5 , Image 6 It can be seen from the figure that the peak positions of the volatile components of tobacco leaves from different origins are roughly similar, but the peak contents are quite different.

[0058] Through the tandem search of the chemical workstation NIST and WILEY mass spectral library, using the retention index and references to assist the mass spectral search for qualitative identification, Hunan, Guizhou, Yunnan and Zimbabwe identified 24, 28, 27 and 24 volatile components, respectively. The specific results are shown in Table 1. , Table 2, Table 3, Table 4.

[0059] Table 1

[0060]

[0061]

[0062]

[0063] Table 2

[0064]

[0065]

[0066] table 3

[0067]

[0068]

[0069]

[0070] Table 4

[0071] ...

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Abstract

The invention discloses a method for detecting volatile components in tobacco leaves. The method comprises the following steps: (1), mixing tobacco leaves with water, so as to obtain a mixed liquor; (2), suspending a solid phase microextraction device above the mixed liquor and carrying out headspace solid-phase micro-extraction, so as to obtain an extract liquor; during the process of headspace solid-phase micro-extraction, performing microwave processing on the mixed liquor, wherein the microwave power is 500 to 700W; (3), carrying out gas chromatography-mass spectrum association analysis, so as to obtain the detecting results of the volatile components in tobacco leaves. According to the method for detecting the volatile components in tobacco leaves, microwave-assisted headspace solid phase microextraction technology is used for analyzing volatile chemical components in tobacco leaves from different places of origin, the speed is fast, the efficiency is high, the sensitivity is high, and the analysis and detection results are comprehensive.

Description

technical field [0001] The invention relates to the field of analysis and detection of tobacco leaves, in particular to a method for detecting volatile components in tobacco leaves. Background technique [0002] The aroma of tobacco is the result of the joint action of various aroma components with specific aroma characteristics. The composition, content and proportion of aroma components of different tobacco types or varieties of the same tobacco type under different ecological environments and cultivation conditions are different. are not all the same and therefore exhibit different aroma types. Through qualitative and quantitative analysis, not only can we understand the influence of aroma components on the style of cigarette products, but also provide technical basis and reference information for cigarette formulation designers and flavoring technicians. [0003] At present, there are many pretreatment and detection methods for the volatile aroma components of tobacco l...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 周国俊杨艳芹储国海袁凯龙蒋健史春云刘金莉夏倩卢昕博胡雅军
Owner CHINA TOBACCO ZHEJIANG IND
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