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Method for promoting germination of caulis sinomenii seeds

A kind of technology of seeds and vines, which is applied in the field of promoting the germination of vines, can solve the problems of dormancy, restriction of the expansion of vines, airtightness, etc., to improve the germination rate, solve the problems of dormancy and susceptibility to bacteria Effect

Active Publication Date: 2015-09-30
HUNAN AGRICULTURAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the seeds of Qingfengteng seeds are thick, impermeable and airtight, and there are dormancy problems, and it is difficult to germinate under natural conditions (the untreated germination rate is <1%), which greatly limits the propagation of Qingfengteng

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] In the middle and late October, pick the ripe drupes of Qingfengteng in that year, let them stand in the container and ripen them for 3 days, then remove the pulp and clean the seeds, collect the seeds that sink to the bottom of the water and dry them in the sun; take 50 sun-dried seeds After washing with clean water, soak the seeds with 100ppm gibberellin solution for 48 hours at room temperature, and then open the inside of the half-moon shape of the seeds; put the broken shells of A. chinensis seeds into a mixture of 2g / L carbendazim + 1g / L penicillin sodium Soak in aqueous solution for 1 hour, then soak in alcohol with 70% mass volume concentration for 30 seconds, then treat with 0.1% mercuric chloride for 5 minutes, rinse with sterile water 5 times; spread the sterilized seeds evenly on sterilized agarose culture On the base, germination was accelerated in a light incubator under the conditions of 22-28°C, light intensity of 2000Lx, and light of 12 hours / d; after 30...

Embodiment 2

[0020] In the middle and late October, pick the ripe drupes of Qingfengteng in the current year, let them stand in the container and ripen them for 2 days, then remove the pulp and clean the seeds, collect the seeds that sink to the bottom of the water and dry them in the sun; take 50 sun-dried seeds After washing with clean water, soak the seeds with 200ppm gibberellin solution for 36 hours at room temperature, and then open the inner side of the half-moon shape of the seeds; put the broken shelled Caulis chinensis seeds into a mixture of 2g / L carbendazim + 0.5g / L penicillin sodium Soak in the mixed aqueous solution for 1 hour, then soak in 70% mass volume concentration of alcohol for 30 seconds, then treat with 0.1% mercuric chloride for 5 minutes, rinse with sterile water 5 times; spread the sterilized seeds evenly on sterilized agarose On the culture medium, germination was accelerated in a light incubator under the conditions of 22-28°C, light intensity of 2000Lx, and ligh...

Embodiment 3

[0022] In the middle and late October, pick the ripe drupes of Qingfengteng in the current year, let them stand in the container and ripen them for 2 days, then remove the pulp and clean the seeds, collect the seeds that sink to the bottom of the water and dry them in the sun; take 50 sun-dried seeds After washing with clean water, soak the seeds with 200ppm gibberellin solution for 24 hours at room temperature, and then open the inner side of the half-moon shape of the seeds; put the broken shelled Caulis chinensis seeds into a mixture of 4g / L carbendazim + 0.5g / L penicillin sodium Soak in the mixed aqueous solution for 0.8 hours, then soak in 70% mass volume concentration of alcohol for 30 seconds, then treat with 0.1% mercuric chloride for 3 minutes, rinse with sterile water 4 times; spread the sterilized seeds evenly on sterilized agarose On the culture medium, germination was accelerated in a light incubator under the conditions of 22-28°C, light intensity of 2000Lx, and 1...

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Abstract

The invention discloses a method for promoting the germination of caulis sinomenii seeds, and belongs to the field of plant breeding. The method comprises the following steps: collecting mature fruits of caulis sinomenii in mid-to-late October for standing till the maturity is improved to a certain extent; removing fruit pulp and cleaning the seeds, and selecting the seeds falling to the water bottom for drying in the sun; sufficiently soaking the dried seeds in a gibberellins solution; cutting the soaked seeds to ensure that openings are formed in the inner sides of the soaked seeds, carrying out disinfection, and then inoculating the disinfected seeds onto a sterile culture medium or sand; carrying out germination culture in an illumination incubator (the culture temperature is 25+ / -3 DEG C, the illumination strength is 2000 Lx, and the illumination cycle is 12 hours per day), so that the seed germination rate exceeds 60%. Through the adoption of the method, not only is the germination rate improved, but also disease and pest sources in the caulis sinomenii seeds can be removed through disinfection and other procedures, so that the problem that field diseases and pests are caused because the disease and pest sources in the caulis sinomenii seeds are brought into seedlings can be solved.

Description

technical field [0001] The invention belongs to the field of plant breeding, and relates to a method for promoting the germination of chrysanthemum chinensis seeds. Background technique [0002] Qingfengteng, also known as Qingfengteng, Xunfengteng, Qingteng, can dispel rheumatism, dredge meridians, and facilitate urination. The 2010 edition of "The Pharmacopoeia of the People's Republic of China" stipulates that Qingfeng Fujiwara is a plant of the genus Fangji in the family Fangji. Sinomenium acutum (Thumb) Rehd.et Wils and Mao Qingteng Sinomenium acutum (thumb) Rehd. et Wils. Var cinereum Rehd. Et Wils Both. [0003] Qingfengteng is often used for rheumatic arthralgia, joint swelling, paralysis and itching; it is also used for the treatment of glomerular diseases, hyperosteogeny, and myelitis; it is also used for the treatment of arrhythmia, lupus erythematosus and other diseases and heroin dependence. It is clinically used to treat rheumatic diseases with its sing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02A01C1/08
Inventor 杨华曾维军熊兴耀吴飞驰仇萍黄宇明何莹李辉
Owner HUNAN AGRICULTURAL UNIV