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Biologically active external dressing and preparation method thereof

A technology of biological activity and raw materials, applied in the field of biomedical engineering, can solve the problem of short biological activity retention time, and achieve the effects of low price, shortening the wound healing process, and low immunogenicity

Active Publication Date: 2018-11-30
CHONGQING ACAD OF ANIMAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, FGF is a biologically active substance, which has a half-life in aqueous media, and its biological activity is maintained for a short time.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Mixing of FGF and silk fibroin

[0025] Take 10 grams of silk fibroin derived from the posterior silk gland of silkworm, dissolve it in 100 ml of three-distilled water, add 20,000 active units of streptomycin and penicillin, add 1 microgram of fibroblast growth factor (FGF), and cool down (0 -8°C) for 1 hour to prepare the mixture of FGF and silk fibroin.

[0026] (2) Dehydration of the mixture

[0027] The mixture of FGF and silk fibroin prepared in step (1) was placed in a petri dish, and freeze-dried to form a film. That is, a biologically active external dressing is prepared.

[0028] (3) Storage and transportation

[0029] Take 1 gram of the film-like material prepared in step (2), pack it in a vacuum-sealed package, store and transport it at low temperature (0-8°C), and have a shelf life of 6 months.

[0030] (4) How to use

[0031] The invention is a medical product for external use, which is a dressing suitable for minor body surface wounds and sutured ...

Embodiment 2

[0033] In this example, non-silk gland-derived silk fibroin was used as raw material.

[0034] (1) Mixing of FGF and silk fibroin

[0035] Take 10 grams of silk fibroin, dissolve it in 100 ml of three-distilled water, add 20,000 active units each of streptomycin and penicillin, add 1 microgram of fibroblast growth factor (FGF), and let stand at low temperature (0-8°C) for 1 Hours, the mixture of FGF and silk fibroin is made.

[0036] (2) Dehydration of the mixture

[0037] The mixture of FGF and silk fibroin prepared in step (1) was placed in a petri dish, and freeze-dried to form a film. That is, a biologically active external dressing is prepared.

[0038] (3) Storage and transportation

[0039] Pack 1 gram of the film-like material prepared in step (2) in a vacuum-sealed package, store and transport at low temperature (0-8°C), and have a shelf life of 6 months.

[0040] (4) How to use

[0041] The invention is a medical product for external use, which is a dressing su...

Embodiment 3

[0043] (1) Mixing of FGF and silk fibroin

[0044] Take 25 grams of silk fibroin derived from the posterior silk gland of silkworm, dissolve it in 100 ml of three-distilled water, add 30,000 active units of streptomycin and penicillin, add 5 micrograms of fibroblast growth factor (FGF), and cool down (0 -8°C) for 1 hour to prepare the mixture of FGF and silk fibroin.

[0045] (2) Dehydration of the mixture

[0046] The mixture of FGF and silk fibroin prepared in step (1) was placed in a petri dish, and freeze-dried to form a film. That is, a biologically active external dressing is prepared.

[0047] (3) Storage and transportation

[0048] Pack 1 gram of the film-like material prepared in step (2) in a vacuum-sealed package, store and transport at low temperature (0-8°C), and have a shelf life of 6 months.

[0049] (4) How to use

[0050] The invention is a medical product for external use, which is a dressing suitable for minor body surface wounds and sutured surgical woun...

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Abstract

The invention belongs to the field of biomedical engineering, and particularly relates to an external dressing with biological activity prepared from silk fibroin and fibroblast growth factors as raw materials, and a preparation method of the external dressing. The external dressing with biological activity provided by the invention is prepared by the following steps: dissolving silk fibroin into water, adding streptomycin, penicillin and the fibroblast growth factors, mixing evenly, and standing at 0-8 DEG C for 30-120 minutes, so as to prepare stoste with biological activity; freezing and drying the stoste to obtain a bioactive material; and dissolving the bioactive material into gel with normal saline to obtain the external dressing. The external dressing with the biological activity is in a hydrogel form; the wound healing process of a patient can be effectively shortened by application of the FGF (fibroblast growth factors); and the external dressing can effectively play a role of the FGF in promoting wound repairing after being coated on the wound, and is convenient to use.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and in particular relates to a biologically active external dressing made from silk fibroin and fibroblast growth factor and a preparation method thereof. Background technique [0002] Open wounds on the human body surface generally involve damage to tissues and organs such as the skin, subcutaneous tissue, and muscles. How to promote the healing of open traumatic wounds on the human body surface is one of the most common problems in daily life. For the solution of this problem, there are many programs in surgical clinic. However, with the development of society, people have never-ending demands on the quality of medical care. For cases handled by medical institutions such as hospitals, trauma healing is basically guaranteed. However, for some relatively minor injuries, people generally choose to heal themselves for reasons such as saving treatment costs. Therefore, seeking treatments th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/32A61L15/44A61L15/42
Inventor 曾秀周婵黄勇刘彬王介平吕金凤王海燕
Owner CHONGQING ACAD OF ANIMAL SCI