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Multicomponent simultaneous assay method and fingerprint spectrum construction method of callicarpa kwangtungensis and applications thereof

A determination method and fingerprint technology, which are applied in the field of simultaneous determination of multiple components of Guangdong Zizhu, which can solve the problems of complex components and so on.

Inactive Publication Date: 2015-10-14
JIANGSU KANION PHARMA CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to solve the technical problem that the ingredients of Guangdong Zizhu traditional Chinese medicine are extremely complex, and only relying on character identification and content determination is not enough to fully control the quality of Guangdong Zizhu medicinal material, and a Guangdong Zizhu multi-component simultaneous Determination method and fingerprint spectrum construction method and application

Method used

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  • Multicomponent simultaneous assay method and fingerprint spectrum construction method of callicarpa kwangtungensis and applications thereof
  • Multicomponent simultaneous assay method and fingerprint spectrum construction method of callicarpa kwangtungensis and applications thereof
  • Multicomponent simultaneous assay method and fingerprint spectrum construction method of callicarpa kwangtungensis and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Take the same batch of medicinal material powder (Batch S1, Guangdong Zizhu, one year old, produced in Pingxiang Base, collected on 2011.10.21), passed through a 50-mesh sieve, weighed 1.0g accurately, put it in a 25mL volumetric flask, and added 10mL of 50% methanol aqueous solution Weigh it, extract it with ultrasound for 30 minutes (150W, 80Hz), let it cool, weigh it, make up the missing weight with 50% methanol aqueous solution, shake well, and let it stand again. Before use, filter with a 0.22 μm filter membrane, and take the subsequent filtrate as the test solution.

[0046] Using LC-20AD ultra-fast liquid chromatography (UFLC), column Welch Material C 18 Column (2.1mm×100mm, 1.8μm), the mobile phase is acetonitrile (A)-0.5% phosphoric acid aqueous solution (B), and the gradient elution is as follows:

[0047] time (minutes)

Mobile phase (v / v%)

0～10

5~15% mobile phase A and 85~95% mobile phase B

10～20

Linear gradient change to: ...

Embodiment 2

[0066] (1) Take the powder of batches of medicinal materials from S1 to S10, pass through 45 meshes, accurately weigh 1.0g, put it into a 25mL volumetric flask, add 50mL of 70% methanol aqueous solution to weigh it, extract it by ultrasonic for 30 minutes (150W, 80Hz), let it cool , Weigh the weight, make up the missing weight with 70% methanol aqueous solution, shake well, and let stand again. Filter with a 0.22 μm filter membrane before use, and take the subsequent filtrate as the test solution (S1~S10 batches of test solution);

[0067] Using LC-20AD ultra-fast liquid chromatography (UFLC), column Welch Material C 18 Column (2.1mm×100mm, 1.8μm), the mobile phase is acetonitrile (A)-0.5% phosphoric acid aqueous solution (B), and the gradient elution method is as follows:

[0068] time (minutes)

Mobile phase (v / v%)

0～10

8-15% mobile phase A and 85-92% mobile phase B

10～20

Linear gradient change to: 15-17% mobile phase A and 83-85% mobile ph...

Embodiment 3

[0081] (1) Take 16 batches of medicinal material powder (S1-S16), pass through 80 meshes, accurately weigh 1.0g, put in a 25mL volumetric flask, add 10mL of 50% methanol aqueous solution to weigh, and ultrasonically extract for 30 minutes (150W, 80Hz). Let it cool, weigh it, make up the missing weight with 50% methanol aqueous solution, shake well, and let it stand again. Filter with a 0.22 μm filter membrane before use, and take the subsequent filtrate as the test solution (S1~S16 batches of test solution);

[0082] Using LC-20AD ultra-fast liquid chromatography (UFLC), column Welch Material C 18 Column (2.1mm×100mm, 1.8μm), the mobile phase is acetonitrile (A)-0.5% phosphoric acid aqueous solution (B), and the gradient elution is as follows:

[0083] time (minutes)

Mobile phase (v / v%)

0～10

8-15% mobile phase A and 85-92% mobile phase B

10～20

Linear gradient change to: 15-17% mobile phase A and 83-85% mobile phase B

20～30

Linear ...

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Abstract

The invention discloses a multicomponent simultaneous assay method and a fingerprint spectrum construction method of callicarpa kwangtungensis and applications thereof. The assay method includes the steps of performing ultrafast liquid chromatographic detection and high-separation-degree fast liquid chromatographic detection or ultra-high performance liquid chromatographic method to a sample solution of the callicarpa kwangtungensis. The fingerprint spectrum construction method of the callicarpa kwangtungensis includes the steps of (1) detecting the callicarpa kwangtungensis with the detection method, and (2) generating a fingerprint spectrum of the callicarpa kwangtungensis with similarity evaluation software. The assay method of the callicarpa kwangtungensis is simple in operation, is excellent in stability, precision and repeatability, is good in chromatographic peak separation degree of the callicarpa kwangtungensis and is short in analysis time. The obtained fingerprint spectrum of the callicarpa kwangtungensis can be used for controlling the quality of the callicarpa kwangtungensis comprehensively so that quality controlling of the callicarpa kwangtungensis is more comprehensive and scientific.

Description

technical field [0001] The invention relates to a method for simultaneously measuring multiple components of Guangdong purple pearl, a method for constructing a fingerprint spectrum and an application thereof. Background technique [0002] Fingerprints can be represented by common peaks of different characteristics, which can basically reflect the chemical composition and content distribution of traditional Chinese medicines. The United States, the United Kingdom, India, Germany, Canada and other countries have accepted the quality control method of fingerprints. The components represented by each peak in the fingerprints do not require a clear chemical structure, nor do they need to be identified. Each component represented in the fingerprint is clearly quantified, and only the fingerprints of different batches of the same drug product need to be consistent. For example, the standard formulated by Germany and France for its Ginkgo biloba standard preparation EGb761 not onl...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 杨义芳李丽胡晓
Owner JIANGSU KANION PHARMA CO LTD
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