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Detection method of glyphosate pesticide based on nematode avoidance behavior

A detection method, glyphosate technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high cost, low sensitivity, complicated operation, etc., and achieve the effect of low cost, easy to master, and convenient experimental operation

Inactive Publication Date: 2017-06-13
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a glyphosate pesticide detection method based on nematode avoidance behavior in order to solve the problems of low sensitivity, complicated operation and high cost in the existing glyphosate identification method

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  • Detection method of glyphosate pesticide based on nematode avoidance behavior

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specific Embodiment approach 1

[0011] Specific embodiment one: the glyphosate pesticide detection method based on nematode avoidance behavior in this embodiment is implemented according to the following steps:

[0012] 1. Weigh 2-3g of agar into a conical flask, add 1000mL of pure water, seal it with a parafilm, put it in a high-pressure steam sterilizer for sterilization, cool to 40-50°C and add it to a petri dish The sterilized agar solution is cooled and solidified at room temperature to obtain an agar dish;

[0013] 2. Take 4 observation points equidistantly from the circumference edge of the agar plate obtained in step 1, and drop 1 μL of the pesticide to be tested and 1 μL of E. and 1 μL of pure water, add 80 to 200 Tretocephalus nematode L2 larvae at the fourth observation point;

[0014] 3. After 1.5 to 2.5 hours of observation, when more than 30% of Tretocephalus nematode L2 stage larvae gather at the first observation point, it indicates that the pesticide to be tested contains glyphosate.

[00...

specific Embodiment approach 2

[0016] Embodiment 2: This embodiment differs from Embodiment 1 in that the sterilization treatment in step 1 is sterilized at 120° C. for 20 minutes. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0017] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that the diameter of the petri dish described in step 1 is 6 cm. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

A glyphosate pesticide detection method based on nematode avoidance behavior, the invention relates to a glyphosate pesticide detection method, which aims to solve the problems of low sensitivity, complicated operation and high cost in the existing glyphosate identification method. Detection method: agar is placed in an Erlenmeyer flask, sealed with a parafilm, sterilized, placed in a petri dish after sterilization, and solidified to obtain an agar dish; 2. Take 4 observation points on the surface of the agar dish, add The pesticide to be tested, Escherichia coli bacteria liquid, pure water and L2 stage larvae of Tretocephalus nematode; 3. When more than 30% of the larvae are gathered in the pesticide to be tested, it indicates that the pesticide contains glyphosate. The detection method of the invention has low requirements on experimental conditions, does not need special high-precision analysis instruments and detection equipment, is convenient to operate, and has high detection sensitivity.

Description

technical field [0001] The invention relates to a detection method of glyphosate pesticide. Background technique [0002] Rapid detection of pesticides is an increasingly required technique in the context of food safety and environmental protection. Nematodes can sense changes in the surrounding environment more sensitively, and can indicate environmental changes in a short period of time. Nematode is widely used as a typical model organism in the field of scientific research because of its easy preservation and convenient experimental operation. The larvae of nematodes are divided into four stages, namely L1, L2, L3 and L4 stages. Nematodes in different growth stages have special tolerance and sensitivity to corresponding environmental stress, from "IUBMB Life" in 2009 "Understanding the mechanism of the dormant dauer formation of C. elegans: from genetics to biochemistry" by Yunbiao Wang et al. [0003] According to the record titled "Research Progress of Glyphosate Res...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/00
Inventor 王云彪阿娜莎陈展彦武海涛
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S