Colloidal gold immunochromatography test paper strip for detecting tetracycline drugs, and preparation method thereof
A technology of tetracycline drugs and immunochromatographic test strips, which is applied in measurement devices, analytical materials, instruments, etc., can solve the problems of being easily affected by other drugs, high price of instruments, poor specificity, etc., and achieve excellent water absorption and release capacity. The effect of improving detection capability and enhancing stability
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Embodiment 1
[0030] like figure 1 As shown, the present invention discloses a colloidal gold immunochromatographic test strip for detecting tetracycline drugs and a preparation method thereof, including a sample pad 1, a binding pad 2, a nitrocellulose coating film 3, a water-absorbing pad 4 and a PVC bottom plate 5 , the sample pad 1, the binding pad 2, the nitrocellulose coating film 3 and the water-absorbing pad 4 are sequentially lapped on the PVC bottom plate 5: the binding pad 2 is coated with a tetracycline Cloned antibody-colloidal gold marker; the nitrocellulose coating 3 is provided with a detection line 31 and a quality control line 32, the detection line 31 is coated with a tetracycline drug antigen, and the quality control line 32 is coated with Goat anti-mouse IgG.
Embodiment 2
[0032] A colloidal gold immunochromatographic test strip for detecting tetracycline drugs and a preparation method thereof, comprising the following steps:
[0033] Step 1. Preparation of colloidal gold: Take a 250 mL beaker, add 100 mL of 0.01% chloroauric acid solution, heat it on a constant temperature magnetic stirrer with a temperature range of 400 °C, and heat it to boiling state. The actual temperature was 100 °C, and after boiling for 30 s, the temperature gear was changed to 200 °C, and the speed gear was adjusted to 200 r / min at the same time, and 500 μL of 4% trisodium citrate solution was quickly added to the beaker, and the solution in the beaker became After heating for 10 minutes after turning red, colloidal gold is obtained;
[0034]Step 2. Preparation and purification of gold-labeled antibody: Take 1 mL of the colloidal gold adjusted to pH 7.9 in a 1.5 mL centrifuge tube, add 9.6 μg / ml tetracycline monoclonal antibody with a dilution factor of 6 to the centrif...
Embodiment 3
[0039] Embodiment 3 performance test
[0040] 3.1 Sensitivity test
[0041] Use 0.01mol / L PBS solution with pH 7.9 to prepare tetracycline, chlortetracycline, and oxytetracycline standard products to different concentrations such as 100 μg / kg, 50 μg / kg, 25 μg / kg, and 15 μg / kg Gradient, and then tested with the assembled colloidal gold immunochromatographic test strips of tetracycline drugs, the results are as follows figure 2 shown.
[0042] Depend on figure 2 It can be seen that when the content of the tetracycline standard substance is 15 μg / L, there is a faint red color on the T line of the test strip by visual observation, and when the content of the tetracycline standard substance is 20 μg / L, the test strip The paper strip shows a line (quality control line), so the detection limit of the test strip for the tetracycline standard is 20 μg / L; when the content of the chlortetracycline standard is 15 μg / L, the test paper can be observed by naked eyes. There is also a fa...
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