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Phosphorylated modification method for improving emulsibility of ovalbumin

A technology of ovalbumin and phosphorylation, which is applied in the direction of animal protein processing, etc., to achieve the effect of promoting application, simple processing method and improving emulsification.

Active Publication Date: 2015-10-21
安徽荣达食品有限公司
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AI Technical Summary

Problems solved by technology

However, its suboptimal emulsifying properties limit its application in emulsifying systems

Method used

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  • Phosphorylated modification method for improving emulsibility of ovalbumin
  • Phosphorylated modification method for improving emulsibility of ovalbumin
  • Phosphorylated modification method for improving emulsibility of ovalbumin

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Embodiment Construction

[0016] In order to make the content of the present invention easier to understand, the technical solutions of the present invention will be further described below in conjunction with specific embodiments, but the present invention is not limited thereto.

[0017] 1 Instruments and reagents

[0018] 1.1 Experimental Instruments

[0019] Model 722 visible spectrophotometer (Shanghai Sunny Hengping Scientific Instrument Co., Ltd.); FJ200-SH digital high-speed dispersion homogenizer (Shanghai Specimen Model Factory); centrifuge (Shanghai Anting Scientific Instrument Factory); HJ-3 digital Display constant temperature magnetic stirrer (Changzhou Zhenhua Instrument Co., Ltd.); FD5-2.5 vacuum freeze dryer (SIM, U.S.A), FA2004 electronic balance (Shanghai Sunny Hengping Scientific Instrument Co., Ltd.); HH-S 2 Constant temperature water bath (Jintan Chenghui Instrument Factory); PHSJ-4A laboratory pH meter (Shanghai Precision Scientific Instrument Co., Ltd.); 25mm dialysis bag (BIOS...

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Abstract

The present invention provides a phosphorylated modification method for improving emulsibility of ovalbumin. The method includes the following steps: water is added into the ovalbumin to prepare a protein solution with a mass concentration of 5.0%, then sodium tripolyphosphate with 3.0% the mass of the ovalbumin is added into the protein solution, the pH value is adjusted to be 7.6, then the phosphorylated reaction of the mixed liquid is conducted at 39 DEG C for a reaction time of 153 min; and the supernatant is collected after the completion of the reaction, and freeze-dried to obtain the modified ovalbumin. Compared with the unmodified ovalbumin, the modification method of the ovalbumin can significantly improve the emulsibility of the ovalbumin with an emulsifying activity reaching 0.513, is simple, and can promote the application of the ovalbumin in emulsifying system.

Description

technical field [0001] The invention specifically relates to a method for phosphorylation modification to improve the emulsification of ovalbumin. Background technique [0002] Phosphorylation is the process of adding phosphate groups to intermediate metabolites or proteins. The mechanism is that inorganic phosphoric acid forms -C-O-Pi or -C-N-Pi esterification reactions with specific oxygen atoms or nitrogen atoms on proteins, thereby introducing Phosphate group can increase the electrostatic repulsion between protein molecules, increase the electronegativity of protein, make it easier to disperse, and improve the emulsification of protein. Moreover, phosphorus atom is an important component of biofilm and one of the indispensable elements of all organisms. From a toxicological point of view, phosphate ion is necessary for all biological metabolism, but it must be obtained from diet, so protein Phosphorylation modification is a practical and effective method. [0003] Ova...

Claims

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Application Information

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IPC IPC(8): A23J3/04
Inventor 黄群宋洪波滕慧王艺伟吴枭锜许美玉
Owner 安徽荣达食品有限公司
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