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A kind of Trichoderma harzianum jsnl1404 and its application

A technology of microbial strains and inoculants, applied in the application, chemicals for biological control, biocides, etc., can solve the problems of root rot, economic loss, leaves, young shoots withering and yellowing, and achieve easy preservation, resistance to The effect of strong inversion ability and simple cultivation conditions

Active Publication Date: 2017-12-19
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, for more than ten years, with the continuous expansion of the artificial cultivation area of ​​Dendrobium candidum, the occurrence of diseases is common and serious. Under warm and humid environmental conditions, the occurrence of Sclerotium rolfssii is more common, which can cause root rot. Leaves and young shoots wither and die, which is a devastating disease that causes huge economic losses. For this reason, it is of practical significance to carry out the prevention and treatment of Dendrobium officinale

Method used

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  • A kind of Trichoderma harzianum jsnl1404 and its application
  • A kind of Trichoderma harzianum jsnl1404 and its application
  • A kind of Trichoderma harzianum jsnl1404 and its application

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Effect test

Embodiment 1

[0040] The characteristics of embodiment 1 Trichoderma harzianum (Trichoderma harzianum) JSNL1404 bacterial strain

[0041] The morphology and physiological activity characteristics of Trichoderma harzianum (Trichoderma harzianum) JSNL1404 described in the present invention are as follows:

[0042] Trichoderma harzianum (Trichoderma harzianum) JSNL1404 grows fast on the PDA medium plate of the PRX-250A type intelligent artificial climate box at 25 ± 1 ℃ (RH70%, dark), and the average daily growth is 2.7cm. White, fading to light green. The optimum temperature is 30°C, and it grows better at 35°C. Conidia are subspherical to ovoid, smooth surface, light green, 2.8-3.2×2.5-2.8μm in size.

[0043] The mycelia of Trichoderma harzianum JSNL1404 grew best at 25±1°C and pH 4-6, with an average daily growth of 2.6 cm; at 30°C, the sporulation was the highest at pH 6-8. The optimum growth temperature of the bacteria is 25±1℃.

[0044] The rDNA-ITS sequence of Trichoderma harzianum ...

Embodiment 2

[0046] The preparation method of Trichoderma harzianum (Trichoderma harzianum) JSNL1404 inoculum: Inoculate Trichoderma harzianum JSNL1404 in liquid fermentation medium, pH 4.5, liquid volume in 500mL Erlenmeyer flask is 250mL, 25°C-30°C, shaker speed 120 revs / min, 72 hours, the fermented liquid that obtains is the original bacterium liquid of preventing and treating Dendrobium candidum candidum.

[0047] Wherein the formula of liquid fermentation medium is: 1000mL distilled water, 120g potato, 15g sucrose, 0.5g ammonium sulfate, pH4.5.

Embodiment 3

[0049] The preparation method of Trichoderma harzianum (Trichoderma harzianum) JSNL1404 inoculum: inoculate Trichoderma harzianum JSNL1404 in liquid fermentation medium, pH 5.5, liquid volume in 500mL Erlenmeyer flask is 250mL, 25°C-30°C, shaker speed 160 revs / min, 120 hours, the fermented liquid that obtains is the original bacterium liquid of preventing and treating Dendrobium candidum candidum.

[0050] Wherein the formula of liquid fermentation medium is: 1000mL distilled water, 150g potato, 18g sucrose, 1.0g ammonium sulfate, pH5.5.

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Abstract

The invention discloses a Trichoderma harzianum JSNL1404, which is named Trichoderma harzianum (Trichoderma harzianum). No.9281. The invention also discloses the application of the above bacterial strain. Trichoderma harzianum JSNL1404 of the present invention is mainly used for preventing and treating Dendrobium candidum officinale, and also has inhibitory effect on various plant pathogenic bacteria, and plays a very important role in the biological control of plant diseases by antagonizing fungi, for example, there are many types and quantities; The mode of action of pathogenic bacteria is diversified; the propagation speed is fast; it can be artificially cultivated, easy to operate, convenient for production and application; strong resistance to stress and other advantages, especially the control effect on Dendrobium officinale caused by fungi.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to a Trichoderma harzianum JSNL1404 and application thereof. Background technique [0002] Trichoderma is a fungus that widely exists in nature, and Trichoderma harzianum is the most widely used species of Trichoderma. Trichoderma (Trichoderma spp.) belongs to the subphylum Deuteromycetes, the class Hyphomycetes, the order Polychoderma, and the family Polypodiaceae. Widely present in soil, root circle, leaf circle, seeds and other ecological environments, it is also an excellent biocontrol bacteria. In 1932, Weindling discovered that Trichoderma lignorum can parasitize a variety of plant pathogenic fungi, and suggested that the fungus be used for the biological control of soil-borne plant diseases, and the research on the biocontrol of Trichoderma began from then on. Due to its wide adaptability, broad spectrum and multi-mechanism, Trichoderma has always been the focus of research ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P1/00A01P3/00C12R1/885
CPCA01N63/30C12N1/14C12N1/145C12R2001/885
Inventor 赵桂华谢春芹赵楠杨鹤同
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY