Macrofungi specimen preparation method

A technology of specimen making and fungi, applied in the fields of botany equipment and methods, teaching models, instruments, etc., can solve problems such as environmental pollution of specimen preservation and storage, and achieve the effect of solving environmental pollution and inhibiting the growth of microorganisms.

Inactive Publication Date: 2015-10-28
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for preparing large fungi specimens, aiming to solve the problem ...

Method used

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  • Macrofungi specimen preparation method

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Embodiment 1

[0016] S101: Collect Pleurotus ferulae with normal growth and no pests and diseases as specimens. It is required to preserve the integrity of the strain as much as possible and keep its classification and identification characteristics. After the collected fresh Pleurotus ferulae is washed with tap water, use filter paper to dry the water stand-by;

[0017] S102: Soak the Pleurotus ferulae specimen in S101 in the treatment solution (200ml of formaldehyde, 30g of potassium nitrate, 30g of potassium acetate, and add distilled water to 1000ml) for 2 hours; take out the soaked specimen, and absorb the excess treatment solution on the surface of the specimen with filter paper ;

[0018] S103: Soak the pretreated Pleurotus ferulae specimen in 75% ethanol for 2 hours, and then soak in 95% ethanol solution for 2 hours to complete the dehydration process; put the dehydrated specimen into plasticizing reagent B Plasticize in medium for 15 minutes, remove excess plasticizing reagent B o...

Embodiment 2

[0033] S105: Collect Agaricus blazei with normal growth and no pests and diseases as specimens. It is required to preserve the integrity of the strain as much as possible and keep its classification and identification characteristics. After the collected fresh Agaricus blazei are washed with tap water, use filter paper to dry the water and set aside ;

[0034] S106: Soak the Agaricus blazei specimen in S101 in the treatment solution (200ml of formaldehyde, 30g of potassium nitrate, 30g of potassium acetate, and add distilled water to 1000ml) for 2 hours; take out the soaked specimen, and absorb the excess treatment solution on the surface of the specimen with filter paper;

[0035]S107: Soak the pretreated Agaricus blazei specimen in 75% ethanol for 2 hours, and then soak in 95% ethanol solution for 2 hours to complete the dehydration process; put the dehydrated specimen into plasticizing reagent B Plasticize for 15 minutes, remove excess plasticizing reagent B on the surface ...

Embodiment 3

[0050] S109: Collect tea tree mushrooms that grow normally and are free from diseases and insect pests as specimens. It is required to preserve the integrity of the strain as much as possible and keep its classification and identification characteristics. After the collected fresh tea tree mushrooms are washed with tap water, use filter paper to dry the water and set aside ;

[0051] S110: Soak the tea tree mushroom specimen in S101 in the treatment solution (200ml of formaldehyde, 30g of potassium nitrate, 30g of potassium acetate, and add distilled water to 1000ml) for 2 hours; take out the soaked specimen, and absorb the excess treatment solution on the surface of the specimen with filter paper;

[0052] S111: Soak the pretreated tea tree mushroom specimen in 75% ethanol for 2 hours, then soak in 95% ethanol solution for 2 hours to complete the dehydration process; put the dehydrated specimen into plasticizing reagent B Plasticize for 15 minutes, remove excess plasticizing ...

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Abstract

The invention discloses a preparation method of macrofungi specimens. According to the preparation method, a macrofungi specimen is cleaned and undergoes anticorrosion pretreatment; then, the specimen is plasticized with polyethylene glycol; a plasticizer on the surface of the plasticized specimen is sucked dry with filter paper; and finally, prepared transparent urea-formaldehyde resin and the plasticized macrofungi specimen are fused completely. By the preparation method, breeding of microbes in the macrofungi specimen is inhibited effectively, the original color of the macrofungi specimen is maintained effectively, and the problem of environmental pollution during preservation of specimens is solved. The preparation method provided by the invention is suitable for preservation of macrofungi of various colors as well as preservation of macrofungi specimens and opens up a new way for the preparation of macrofungi specimens.

Description

technical field [0001] The invention belongs to the technical field of fungal specimens, in particular to a method for preparing large fungal specimens. Background technique [0002] Large fungi have high water content, are easy to rot, and are prone to insects, which is difficult to handle and preserve specimens. The traditional production methods of large-scale fungal display specimens mainly adopt two methods: dipping and drying. The dipped specimens must be stored in a volatile preservation solution containing formaldehyde, ethanol, glacial acetic acid, etc. for a long time, but the specimens are easily corroded by the formaldehyde solution. The body of the specimen is easy to harden and become brittle, easy to fade, not fresh, and the preservation solution is easily turbid, so the preservation solution must be replaced frequently, and formaldehyde is volatile and harmful to the human body. Dried specimens are dried in the sun, dried or air-dried. The specimens are seve...

Claims

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Application Information

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IPC IPC(8): A01N3/00G09B23/00G09B23/38
Inventor 吴小杰李峻志祁鹏陈世明李安利马小魁戴璐张黎光
Owner MICROBIOLOGY INST OF SHAANXI
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