Two-step production method for microalgae bio-oil

A bio-oil and microalgae technology, which is applied in the preparation method of peptides, fat oil/fat production, chemical instruments and methods, etc., can solve the problems of high nitrogen content and high cost of quality improvement, and achieve the reduction of nitrogen content, reduction of catalyst poisoning, The effect of saving consumption

Active Publication Date: 2015-10-28
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a two-step method for producing microalgae bio-oil, wh

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The method for producing microalgae bio-oil by the two-step method in this embodiment includes a protein extraction step and a hydrothermal liquefaction method for producing microalgae bio-oil: wherein,

[0053] Protein extraction steps include the following processes:

[0054] First, take the chlorella algae mud whose dry weight of algae is 150g, put it into a grinding cup, and grind it for 24 hours at a speed of 150rpm to obtain a slurry; and the oil content of chlorella is 9.18%, and the protein content of chlorella is 46.75%, the water content of chlorella algae mud is 92%, that is, the solid content is 8%; the grinding beads in the grinding cup are zirconia with a particle size of 1.8mm-2.4mm, and the volume filling capacity is 50%.

[0055] Then, add 6L of tris-HCl buffer solution with a pH value of 9.0 to the slurry, and add 3g of sodium dodecylsulfonate, the water bath temperature is 50°C, stir and extract for 4h, after cooling, centrifuge to obtain protein aque...

Embodiment 2

[0061] The method for producing microalgae bio-oil by the two-step method in this embodiment includes a protein extraction step and a hydrothermal liquefaction method for producing microalgae bio-oil: wherein,

[0062] Protein extraction steps include the following processes:

[0063] First, take the chlorella algae mud whose dry weight of algae is 150g, put it into a grinding cup, and grind it for 24 hours at a speed of 150rpm to obtain a slurry; and the oil content of chlorella is 9.18%, and the protein content of chlorella is 46.75%, the water content of chlorella algae mud is 80%, that is, the solid content is 20%; the grinding beads in the grinding cup are zirconia with a particle size of 0.3mm-8mm, and the volume filling capacity is 85%.

[0064] Then, add 3L of NaOH aqueous solution with a pH value of 10.0 to the slurry, and add 6g of sodium dodecylsulfonate, the temperature of the water bath is 40°C, stir and extract for 6h, after cooling, separate by air flotation to ...

Embodiment 3

[0068] The method for producing microalgae bio-oil by the two-step method in this embodiment includes a protein extraction step and a hydrothermal liquefaction method for producing microalgae bio-oil: wherein,

[0069] Protein extraction steps include the following processes:

[0070] First, take the chlorella algae mud whose dry weight of algae is 150g, put it into a grinding cup, and grind it for 24 hours at a speed of 150rpm to obtain a slurry; and the oil content of chlorella is 9.18%, and the protein content of chlorella is 46.75%, the water content of chlorella algae mud is 95%, that is, the solid content is 5%; the grinding beads in the grinding cup are zirconia with a particle size of 0.3mm-8mm, and the volume filling capacity is 72%.

[0071] Then, 3 L of NaOH aqueous solution with a pH value of 13 was added to the slurry, and 7.5 g of sodium dodecylsulfonate was added. The temperature of the water bath was 60 ° C, and the extraction was stirred for 2 hours. After coo...

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Abstract

Relating to the technical field of microalgae bio-oil extraction, the invention discloses a two-step production method for microalgal bio-oil to solve the problem of high quality improvement cost caused by large nitrogen content of microalgae bio-oil. The method includes: firstly extracting protein contained in microalgae cells of a microalgae raw material to obtain a protein water solution and microalgae residue; then separating the protein precipitate from the protein water solution to obtain protein and a water phase; then adjusting the solid content of the microalgae residue with the water phase to obtain a microalgae residue suspension; and then taking the microalgae residue suspension as the raw material, and utilizing hydrothermal liquefaction method to produce microalgae bio-oil. Compared with conventional hydrothermal liquefaction methods, the microalgae bio-oil obtained by the method has a nitrogen content reduced by 40%, thus greatly saving the dosage of hydrogen during subsequent microalgae bio-oil quality improvement, reducing catalyst intoxicating phenomenon in the quality improvement process, and also realizing secondary utilization of the water phase at the same time. The two-step production method for microalgal bio-oil provided by the invention is used for extraction of microalgae bio-oil.

Description

technical field [0001] The invention relates to the technical field of microalgae bio-oil extraction, in particular to a two-step method for producing microalgae bio-oil. Background technique [0002] Microalgae (Microalgae) are a class of autotrophic plants that are widely distributed on land and in the ocean and have high photosynthetic utilization. Because microalgae have a certain ability to "repair" the environment, when microalgae is used as raw material to produce microalgae bio-oil (Bio-oil of Microalgae), the purpose of energy production and environmental protection can be achieved at the same time. Therefore, the use of microalgae to produce microalgae Bio-oil methods have received a lot of attention. [0003] Generally, microalgae bio-oil is produced by thermochemical conversion methods such as pyrolysis, hydrothermal liquefaction, or gasification; among them, hydrothermal liquefaction (HTL) is based on the whole microalgae Although the production of microalgae ...

Claims

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Application Information

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IPC IPC(8): C11B1/00C11B1/04C07K1/30
Inventor 杨巧利白秀军杨建强刘敏胜
Owner ENN SCI & TECH DEV
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