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Method for synthesizing L-ascorbic acid-2-glucoside through enzyme fermentation process

A technology of ascorbic acid and glucoside, which is applied in the field of enzyme fermentation, can solve the problems of high environmental hazards, low yield, and non-environmental protection, and achieve the effects of high product purity, stable and uniform crystal form, and less impurities

Inactive Publication Date: 2015-10-28
PROYA COSMETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, L-ascorbic acid-2-glucoside mainly adopts the method of chemical synthesis, which is harmful to the environment, not environmentally friendly, and the yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for synthesizing L-ascorbic acid-2-glucoside by enzyme fermentation, characterized in that the following steps are adopted:

[0019] A. Add pure water to the reaction kettle, then add vitamin C, and start stirring;

[0020] B. Control the internal temperature of the reactor at 37°C, and adjust the pH to 5.50±0.10 with 30% by mass NaOH solution;

[0021] C. Add β-cyclodextrin to the reaction kettle, then add cyclodextrin glycosyltransferase, and add an appropriate amount of pure water;

[0022] D. Control the temperature in the reactor at 35°C, and react in the dark for 10 hours;

[0023] E. Add glucoamylase, control the temperature at 35°C, and react in the dark for 10 hours;

[0024] F, adding pure water to the reaction kettle for rinsing, to obtain a mixed solution after rinsing; centrifuging the mixed solution, and then filtering to obtain a filtrate;

[0025] G. Transfer the obtained filtrate to the reaction kettle, depressurize when the temperature is ≤...

Embodiment 2

[0029] A method for synthesizing L-ascorbic acid-2-glucoside by enzyme fermentation, characterized in that the following steps are adopted:

[0030] A. Add pure water to the reaction kettle, then add vitamin C, and start stirring;

[0031] B. Control the internal temperature of the reactor at 37°C, and adjust the pH to 5.50±0.10 with 30% by mass NaOH solution;

[0032] C. Add β-cyclodextrin to the reaction kettle, then add cyclodextrin glycosyltransferase, and add an appropriate amount of pure water;

[0033] D. Control the temperature in the reactor at 39°C, and react in the dark for 12 hours;

[0034] E. Add glucoamylase, control the temperature at 39°C, and react in the dark for 14 hours;

[0035] F, adding pure water to the reaction kettle for rinsing, to obtain a mixed solution after rinsing; centrifuging the mixed solution, and then filtering to obtain a filtrate;

[0036] G. Transfer the obtained filtrate to the reaction kettle, depressurize when the temperature is ≤...

Embodiment 3

[0040] A method for synthesizing L-ascorbic acid-2-glucoside by enzyme fermentation, characterized in that the following steps are adopted:

[0041] A. Add pure water to the reaction kettle, then add vitamin C, and start stirring;

[0042] B. Control the internal temperature of the reactor at 37°C, and adjust the pH to 5.50±0.10 with 30% by mass NaOH solution;

[0043] C. Add β-cyclodextrin to the reaction kettle, then add cyclodextrin glycosyltransferase, and add an appropriate amount of pure water;

[0044] D. Control the temperature in the reactor at 37°C, and react in the dark for 11 hours;

[0045] E. Add glucoamylase, control the temperature at 37°C, and react in the dark for 11 hours;

[0046] F, adding pure water to the reaction kettle for rinsing, to obtain a mixed solution after rinsing; centrifuging the mixed solution, and then filtering to obtain a filtrate;

[0047] G. Transfer the obtained filtrate to the reaction kettle, depressurize when the temperature is ≤...

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Abstract

The invention relates to a method for synthesizing L-ascorbic acid-2-glucoside through the enzyme fermentation process. The method is characterized by comprising the steps of A, adding the purified water in a reaction vessel, then adding vitamin C and starting stirring; B, controlling the temperature in the reaction vessel at 37 DEG C, and adjusting the pH value in the reaction vessel to be 5.50+ / -0.10 by the NaOH solution of 30% in mass percentage; C, adding beta-cyclodextrin into the reaction vessel, then adding cyclodextrin glucantransferase and replenishing an appropriate amount of purified water at the same time; D, controlling the temperature in the reaction vessel at 37+ / -2 DEG C and reacting in the dark condition for 10-12 hours; E, adding glucoamylase, controlling the temperature in the reaction vessel at 37+ / -2 DEG C and reacting in the dark condition for 10-14 hours; F, filtering; G, concentrating; H, crystallizing and drying to obtain the finished product of L-ascorbic acid-2-glucoside. The obtained L-ascorbic acid-2-glucoside is stable and uniform in crystal form, fewer in impurities, high in product purity, environment-friendly and pollution-free.

Description

technical field [0001] The invention relates to the technical field of enzyme fermentation, in particular to a method for synthesizing L-ascorbic acid-2-glucoside by enzyme fermentation. Background technique [0002] Vitamin C (ascorbic acid) is a commonly used whitening raw material. It can inhibit the increase of pigment, lighten freckles, have a whitening effect, shrink pores, and adjust skin texture. Vitamin C can inhibit the formation of melanin. Once melanin is produced, vitamin C can also make it light. Please take more vitamin C to protect the skin from the prevention of freckles. In addition to whitening, vitamin C can also increase collagen, Adjust the effect of skin texture. [0003] Vitamin C (ascorbic acid) is well known for its brighter, healthier, more youthful skin. Scientific research shows that the efficacy of vitamin C in brightening and whitening skin comes from its ability to inhibit the formation of melanin and reduce melanin. However, applying vita...

Claims

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Application Information

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IPC IPC(8): C12P19/60
Inventor 毕永贤陈斌蒋丽刚
Owner PROYA COSMETICS
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