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Nicotine degrading bacterium Pseudomonas sp. MHJY8 and its screening method and application

A screening method and nicotine technology, applied in the biological field, can solve the problems of low degradation efficiency and few nicotine-degrading bacteria species, and achieve the effects of high degradation activity, large development and application potential, and enrichment of genetic resources.

Inactive Publication Date: 2015-11-11
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] One of the objects of the present invention is to provide a nicotine-degrading bacterium Pseudomonassp.MHJY8 and its screening method and application

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  • Nicotine degrading bacterium Pseudomonas sp. MHJY8 and its screening method and application
  • Nicotine degrading bacterium Pseudomonas sp. MHJY8 and its screening method and application
  • Nicotine degrading bacterium Pseudomonas sp. MHJY8 and its screening method and application

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Embodiment Construction

[0022] Specific embodiments of the present invention will be described in detail below in conjunction with specific drawings. It should be noted that the technical features or combinations of technical features described in the following embodiments should not be regarded as isolated, and they can be combined with each other to achieve better technical effects.

[0023] 1) Materials used for screening

[0024] Soil samples: collected from the tobacco planting base of Luohe Tobacco Company.

[0025] Nicotine-degrading bacteria primary screening medium: yeast extract 2g, yam 4g, K 2 HPO 4 13g, KH 2 PO 4 4g, (NH 4 ) 2 SO 4 0.2g, NaCl5g, agar powder 20g, trace element mixed solution 10ml (MgSO 4 ·7H 2 O1g, MnSO 4 ·H 2 O0.4g, CuCl 2 2H 2 O0.2g, CaCl 2 2H 2 O0.2g, FeSO 4 ·7H 2 O0.02g is all dissolved in 70ml0.1mol / L HCl solution, V8 vegetable juice is settled to 100ml, obtains trace element mixed solution), distilled water is settled to 1000ml, natural pH. The medi...

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Abstract

The invention discloses a nicotine degrading bacterium Pseudomonas sp. MHJY8 (preserved on 27th, Jan., 2015 in China Center for Type Culture Collection under CCTCC NO.M 2015072 and its screening method and application. The screening method of the nicotine degrading bacterium Pseudomonas sp. MHJY8 includes: establishing a culture medium formula to screen the nicotine degrading bacterium Pseudomonas sp. MHJY8, especially adding common yam rhizome in a primary screening medium, and innovating formulation of trace elements. The Pseudomonas sp. MHJY8 screened by a technical system of the invention has high activity on degrading nicotine and is a good wild original strain for mutation breeding; in addition, the Pseudomonas sp. MHJY8 screened from soil is easier for colonization of the soil and is easy to combine with production of tobacco. The Pseudomonas sp. MHJY8 as a functional strain further enriches genetic resources for degrading nicotine wild bacteria and has a great development and application potential.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a nicotine-degrading bacterium Pseudomonassp.MHJY8 and its screening method and application. Background technique [0002] Nicotine, also known as nicotine, is one of the important alkaloids, mainly present in Solanaceae plants. The nicotine content of tobacco accounts for more than 95% of its total alkaloid content, and is one of the key indicators affecting the quality of tobacco. one. The harm caused by nicotine taken into the human body through active or passive smoking is extensive and profound, and it has become a consensus around the world not to advocate or even ban smoking. With the continuous improvement of people's health concerns and the continuous expansion of the scope of application of my country's smoking ban, how to effectively control and reduce the nicotine content in tobacco leaves is an important issue that cannot be avoided by tobacco farmer...

Claims

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Application Information

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IPC IPC(8): C12N1/20A24B15/20C12R1/38
Inventor 孙会忠王小东朱金峰李广良孙明辉
Owner HENAN UNIV OF SCI & TECH