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Method for improving concentration of alcohol substances in fermented product of anaerobic gas-feeding microbes

A microbial fermentation and microbial technology, applied in the direction of fermentation, bacteria, etc., can solve the problems of secondary environmental pollution and complex process, and achieve the effect of increasing value and increasing concentration

Inactive Publication Date: 2015-11-11
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The chemical absorption catalytic method needs to remove the water vapor in the industrial tail gas and recover the organic solvent. The process is very complicated and there is secondary pollution to the environment.

Method used

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  • Method for improving concentration of alcohol substances in fermented product of anaerobic gas-feeding microbes
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  • Method for improving concentration of alcohol substances in fermented product of anaerobic gas-feeding microbes

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Effect test

Embodiment 1

[0031] In the basal culture medium, molybdate ions were not added. After 4 days of constant temperature shaking culture, samples were taken every 24 hours to detect the growth of microorganisms; samples taken on the fourth day were used to detect the concentrations of ethanol, n-butanol and n-hexanol in the fermentation broth by gas chromatography. Strain reached 0.96 in OD600 on the fourth day, and began to enter the decline period on the fourth day ( figure 1 ). On the fourth day, the product concentrations of ethanol, n-butanol and n-hexanol were 0.24, 0.16 and 0.06 g / L ( figure 2 ).

Embodiment 2

[0033] In the basal culture solution, add molybdate ions at a final concentration of 0.1 mg / L. After 4 days of constant temperature shaking culture, samples were taken every 24 hours to detect the growth of microorganisms; samples taken on the fourth day were used to detect the concentrations of ethanol, n-butanol and n-hexanol in the fermentation broth by gas chromatography. The bacterial strain reached 0.79 in OD600 on the third day, and began to enter the decline period on the fourth day ( image 3 ). On the fourth day, the ethanol production was 0.07g / L, n-butanol and n-hexanol could not be detected, and the growth of bacteria was also poor.

Embodiment 3

[0035] In the basal culture solution, add molybdate ions at a final concentration of 1 mg / L. After 4 days of constant temperature shaking culture, samples were taken every 24 hours to detect the growth of microorganisms; samples taken on the fourth day were used to detect the concentrations of ethanol, n-butanol and n-hexanol in the fermentation broth by gas chromatography. The highest concentration of bacterial strain can only reach OD600=0.35 ( Figure 4 ). No alcohols could be detected in the fermentation product.

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Abstract

The inventions aims to provide a cultivation system for improving concentration of alcohol substances in a fermented product of anaerobic gas-feeding microbes, specifically for improving the concentration of the alcohol substances in the fermented product of the anaerobic gas-feeding microbes in a shaking cultivation or continuous air agitation cultivation system by optimizing components in a cultivation medium. A method for improving the fermentation efficiency of the anaerobic gas-feeding microbes is characterized in that the final concentration of molybdate ions in the cultivation medium for fermentation is not higher than 0.1mg / L. By the method, the concentration of alcohols in the fermented product of gas biotransformation can be improved and the value of the bio-fermentation production of gas can be improved effectively.

Description

technical field [0001] The invention belongs to the technical field of microbial gas fermentation, and in particular relates to a culture system for increasing the concentration of alcohols in anaerobic gas microbial fermentation products. technical background [0002] With the rapid development of my country's economy, power plants, steel plants and other industrial and mining enterprises discharge a large amount of industrial tail gas into the atmosphere, which not only causes serious environmental pollution, but also causes a series of environmental problems, and also causes serious harm to human health. This type of industrial tail gas is rich in CO, CO 2 and H 2 , is a kind of cheap raw material for the manufacture of petrochemical chemicals with great development value. How to fully develop and utilize these industrial tail gases can not only reduce environmental pressure, but also form substitute products for petrochemical resources, so as to alleviate my country's ...

Claims

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Application Information

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IPC IPC(8): C12P7/02C12N1/20
Inventor 韩一凡郭亚琼谢彬涛王兴彪王敬敬张小霞黄志勇
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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