Rapid identification method for atrazine residual phytotoxicity and application thereof

An identification method, the technology of atrazine, applied in the field of pesticide detection, can solve the problems of inhibited plant growth, decreased yield, difficult to accurately judge, etc., and achieve the effects of low identification limit, fast result and easy operation

Inactive Publication Date: 2015-11-11
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in order to achieve the expected control effect, farmers will continue to increase the dosage and frequency of pesticide application, which will eventually lead to more serious consequences. Most of the herbicide components are free in the soil, which often leads to the emergence of subsequent crops. Phytotoxicity, often manifested as plant growth inhibitio

Method used

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  • Rapid identification method for atrazine residual phytotoxicity and application thereof
  • Rapid identification method for atrazine residual phytotoxicity and application thereof
  • Rapid identification method for atrazine residual phytotoxicity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0037] Example 1

[0038] (1) Put the sterilized non-phytotoxic cucumber seeds into a seed germination box, and place them in a biochemical incubator at 28-30°C for accelerating germination until the crops germinate about 0.5-1cm in length, and take them out for later use.

[0039] (2) Configuration of atrazine gradient concentration solution: use conventional complete nutrient solution to configure atrazine solution with different concentration gradients 0.009mga.i. / L, 0.09mga.i. / L, 0.45mga.i. / L, 0.9mga.i. / L, 1.8mga.i. / L.

[0040] (3) Take 100 mL of atrazine solution of different concentrations, add 5 germinated seeds, blank culture medium as control, culture in light incubator, culture conditions: temperature day / night: 28 / 25℃, light cycle It is: 12h / 12h, the light intensity is 3500~4000lx.

[0041] (4) After culturing for 5 days, take the crop seedlings out of the light incubator and adapt to the dark for 15-20 minutes, then put them in the measuring box, and use the IMAGING-PAM d...

Example Embodiment

[0042] Example 2

[0043] (1) Put the sterilized non-phytotoxic cucumber seeds into a seed germination box, and place them in a biochemical incubator at 28-30°C for accelerating germination until the crops germinate about 0.5-1cm in length, and take them out for later use.

[0044] (2) Configuration of atrazine gradient concentration solution: use complete nutrient solution to configure atrazine solution with different concentration gradients 0.009mga.i. / L, 0.09mga.i. / L, 0.45mga.i. / L, 0.9 mga.i. / L, 1.8mga.i. / L.

[0045] (3) Take 100mL solutions of different concentrations, add 5 germinated seeds, and place them in a light incubator. Two groups of four repeats, one group for 5 days, one group for 11 days, culture conditions: temperature is daytime / Evening: 28 / 25℃, photoperiod: 12h / 12h, light intensity is 3500~4000lx.

[0046] (4) After culturing for 5 days and 11 days respectively, take the crop seedlings out of the light incubator, measure the root length and hypocotyl length, and o...

Example Embodiment

[0050] Example 3

[0051] (1) Put the sterilized cowpea seeds into a seed germination box, and place them in a biochemical incubator at 28-30°C for accelerating germination until the crops germinate about 0.5-1cm in length, and then take them out for use.

[0052] (2) Configuration of atrazine gradient concentration solution: use complete nutrient solution to configure atrazine solution with different concentration gradients 0.009mga.i. / L, 0.09mga.i. / L, 0.45mga.i. / L, 0.9 mga.i. / L, 1.8mga.i. / L.

[0053] (3) Take 100 mL solutions of different concentrations, add 5 germinated seeds, and place them in a light incubator. Two groups of four repeats, one group for 5 days, one group for 11 days, culture conditions: temperature is day / Evening: 28 / 25℃, photoperiod: 12h / 12h, light intensity is 3500~4000lx.

[0054] (4) After culturing for 5 days and 11 days respectively, take the crop seedlings out of the light incubator, measure the root length and hypocotyl length, and observe the growth of ...

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Abstract

Belonging to the technical field of pesticide detection, the invention discloses a rapid identification method for atrazine residual phytotoxicity and application thereof. The method performs identification by detecting the crop chlorophyll fluorescence intensity change. The method detects and compares two successive Fv/Fm and YII of the crop, when they both decrease, the crop suffers phytotoxicity, and the more serious the damage degree is, the more obvious the decrease is, and the higher is the concentration of atrazine in the growth environment. Also, the minimum concentration of the method for identification of phytotoxicity is 0.09ppm, and compared with the concentration of 0.9ppm of visual identification of phytotoxicity, the lower detection limit is lower. With the characteristics of simplicity, rapidity and accuracy, the method can be used as a new means for diagnosing the existence of atrazine residual phytotoxicity in farmland crops, soil and water. The detection time of the method is short, for farmland crops, only 1 day is needed for identification to obtain the result, and for soil or water without crops, only 6 days are needed for identification of phytotoxicity.

Description

technical field [0001] The invention belongs to the technical field of pesticide detection, and in particular relates to a rapid identification method of atrazine residue damage and its application. Background technique [0002] Atrazine is a s-triazine herbicide, which is mainly used to control various broadleaf weeds and gramineous weeds in crop fields such as corn, sorghum and sugarcane. Because of its high selectivity to corn, sugarcane and other crops, wide herbicidal spectrum, convenient use, and low price, it has become a widely used herbicide in the field. my country began to introduce atrazine in the 1960s. With the increase of the service life and the over-reliance and frequent use of this herbicide, field weeds gradually developed resistance. However, in order to achieve the expected control effect, farmers will continue to increase the dosage and frequency of pesticide application, which will eventually lead to more serious consequences. Most of the herbicide co...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 杨彩宏冯莉田兴山岳茂峰崔烨张纯
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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