A kind of liquid culture method of establishing dse and corn symbiosis system

Abstract

The invention relates to a liquid culturing method for establishing a DSE and corn symbiotic system and belongs to the fields of microbiology, plant nutriology and ecology. The liquid culturing method comprises the steps that aseptic seedlings are cultured through corn B73 seeds with the surfaces disinfected, DSE mycelia are cultured through a PDA culture medium, the corn aseptic seedlings and the DSE mycelia are cultured in an aseptic liquid culture medium together, and a plant-fungi symbiotic system without interference of infectious microbes is established. The liquid culturing method mainly comprises the steps of culturing the core aseptic seedlings, preparing a DSE inoculation agent, establishing DSE and core symbiote and detecting the symbiotic culturing system. The liquid culturing method has the advantages that the DSE and corn aseptic co-culturing liquid system is established, the method is stable and efficient, matrix ingredients are definite and uniform, an ideal model is provided for the further research of the infection mechanism of the DSE and the symbiosis mechanism of the DSE and plants, and meanwhile a method reference is provided for the research of other fungi and symbiotic plants.

Description

technical field [0001] The invention relates to a liquid culture method for establishing a symbiotic system of DSE and corn, belonging to the fields of microbiology, plant nutrition and ecology. Background technique [0002] Dark Septate Endophytes (DSE) has a variety of host plants and a wide ecological distribution. It is an important part of the plant root system, plays an important role in the plant body, and has broad application prospects. At present, the research on DSE is gradually deepening. People are no longer limited to understanding the impact of DSE on plant growth and stress resistance. Further understanding of the infection mechanism of DSE and the symbiotic mechanism of DSE and plants have become research hotspots and difficulties. However, the existing DSE and plant symbiotic culture method is mainly to inoculate sterile seedlings in pots under open or semi-open conditions, which is not only easily contaminated by other bacteria, but also uses humus soil an...

AI Technical Summary

Problems solved by technology

However, the existing DSE and plant symbiotic culture method is mainly to inoculate sterile seedlings in pots under open or semi-open conditions, which is not only easily contaminated by other bacteria, but also uses humus soil and peat soil as the culture medium. , river sand or wheat bran, etc., the nutrient composition is relatively complex, which is not conducive to the systematic research on the biological characteristics of DSE and the symbiotic system, especially when studying the relationship between exogenous substances and symbiotic systems, it is impossible to ensure the completeness of exogenous substances in the culture medium. Uniformity and Effectiveness

In addition, only part of the plant roots are in full contact with DSE in the above solid culture method, resulting in a low colonization rate and poor uniformity of DSE in plant roots