Nostoc sphaeroids kutz oligosaccharide as well as preparation method and application thereof

A technology of oligosaccharide and oligosaccharide of kudzu, applied in the field of natural medicine, can solve the problems of structural complexity of algae polysaccharide, limitations of development and utilization, etc., and achieve the effects of promoting in-depth development and application, prolonging survival time, and improving survival rate.

Inactive Publication Date: 2021-08-10
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the biological activities of algal polysaccharides have been widely reported and have great potential for development, their development and utilization are limited due to the complexity of the structure of algal polysaccharides.

Method used

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  • Nostoc sphaeroids kutz oligosaccharide as well as preparation method and application thereof
  • Nostoc sphaeroids kutz oligosaccharide as well as preparation method and application thereof
  • Nostoc sphaeroids kutz oligosaccharide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The preparation of embodiment 1 kudzu rice polysaccharide

[0045] This embodiment has prepared a kind of kudzu rice polysaccharide, and the specific process is:

[0046] (1) Harvesting: collecting Gexiania algae bodies (1-2mm, 2-3mm, 3-4mm, 4-5mm in size) in different growth stages, cleaning and drying, and obtaining Gexiania in different growth stages rice raw material;

[0047] (2) Pretreatment: Pulverize the dry products of Gexianmi from different sources to obtain Pueraria powder;

[0048](3) Hot water leaching: Weigh 1 part by weight of kudzu powder into the extractor, add 200 parts by weight of water, raise the temperature to 90°C and keep stirring, extract by centrifugation for 2 hours to obtain the first extraction liquid. Add 100 parts by weight of water to the extract, carry out the second extraction, heat up to 90°C and keep stirring, after extraction for 1 hour, centrifuge to collect the second extract, and then combine the two extracts;

[0049] (4) Co...

Embodiment 2

[0052] The preparation of embodiment 2 Gexianmi oligosaccharides

[0053] This embodiment prepared a kind of kudzu rice oligosaccharide, the specific process is:

[0054] (1) Degradation of polysaccharides: take 1 part by weight of the Gexian rice polysaccharide powder prepared in different growth stages in Example 1 and add 100 parts by weight of water to dissolve, then add trifluoroacetic acid (concentrations are 1 mg / mL, 0.5 mg / mL respectively) , and reacted at 100°C for 3h.

[0055] (2) Concentration under reduced pressure: the polysaccharide reaction solution was concentrated under reduced pressure at 40° C. until dry. Subsequently, 5 mL of methanol was added, and concentrated under reduced pressure at 40° C. until dryness, and this step was repeated 3 times.

[0056] (3) Freeze-drying: Add 30 mL of water to the dried polysaccharide hydrolyzate to dissolve the degradation product. Transfer to a beaker and seal the film. Place the beaker in a -20°C refrigerator for free...

Embodiment 3

[0061] A method for preparing Gexianmi oligosaccharides differs from Example 2 only in that hydrochloric acid (concentrations of 1 mg / mL and 0.5 mg / mL, respectively) is used for acid hydrolysis.

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Abstract

The invention discloses nostoc sphaeroids kutz oligosaccharide as well as a preparation method and application thereof. The method for preparing the nostoc sphaeroids kutz oligosaccharide is simple, reasonable, stable, efficient and suitable for large-scale production, meanwhile, the nostoc sphaeroids kutz polysaccharide and oligosaccharide prepared through the scheme can prolong the survival time of caenorhabditis elegans under the oxidative stress condition, the survival rate of the caenorhabditis elegans is increased, the nostoc sphaeroids kutz polysaccharide and oligosaccharide have the in-vivo anti-oxidation effect, the nostoc sphaeroids kutz oligosaccharide can also improve the chemical avoidance index of Huntington chorea disease model caenorhabditis elegans, has a certain neuroprotective effect, and can be applied to adjuvant therapy drugs for nervous system diseases and health-care food with a neuroprotective effect, so a new strategy is provided for prevention and treatment of nervous system diseases and health aging, and the nostoc sphaeroids kutz oligosaccharide is worthy of popularization and application; and deep development and application of natural polysaccharide products can be promoted.

Description

technical field [0001] The invention belongs to the technical field of natural medicines, and in particular relates to a kudzu rice oligosaccharide and its preparation method and application. Background technique [0002] Gexianmi is a kind of blue-green algae that is used for both medicine and food. It was named after Ge Hong, a medical scientist, alchemist, and Taoist scholar in the Eastern Jin Dynasty, and it is also recorded in the memoirs of the last emperor Puyi, "My First Half of Life". "Compendium of Materia Medica Supplements", "Medicinal Properties Test" and "National Chinese Herbal Medicine Compilation" all record that Gexianmi has the effects of improving eyesight and nourishing qi, curing night blindness, relieving heat and clearing intervals, long-term food prolonging life, and eliminating fatigue. Gexianmi was approved as a national new resource food in 2018. [0003] Natural polysaccharides are not only important energy supplies for the human body, but also...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07H3/06C07H1/00C07H1/06A61P25/00A61P25/14A61P39/06A61K31/702A61K31/715A23L33/125
CPCA23V2002/00A61K31/702A61K31/715A23L33/125A61P25/00A61P25/14A61P39/06C07H1/00C07H1/06C07H3/06C08B37/0003A23V2200/322A23V2250/28A23V2250/51
Inventor 黄泽波王雨晴钟国桓胡晶晶唐树平王健
Owner SOUTH CHINA UNIV OF TECH
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