A kind of preparation method of in-tube solid-phase microextraction complex column for extracting phosphopeptides

Abstract

The invention relates to a preparation method of an in-pipe solid phase micro-extraction compound column used for extracting phosphopeptide, which belongs to the field of analytical chemistry. The method comprises the following steps: using methacryloyloxyethyl trimethyl ammonium chloride as a bridge to prepare a titanium dioxide-graphene oxide compound; an organic polymer solution composed of a monomer, a ternary pore forming agent and a compound is injected to capillary with silanized inner wall, the solid phase micro-extraction column possessing an integral column structure is prepared by photopolymerization, and the extraction column is used for enrichment of phosphoeptide. The method can solve the problem of difficult preparation of a titanium dioxide extraction column, has advantages of simple operation and flexible regulation and control, and is suitable for selectivity extraction of complex phosphopeptide, and has certain application prospect on analysis of phosphoeptide in proteomics.

Description

technical field [0001] The invention relates to a titanium dioxide / graphene composite preparation technology, which is applied to the preparation of solid-phase microextraction columns in tubes, realizes the extraction and analysis of phosphopeptides, and uses ultraviolet detectors for detection, and belongs to the field of analytical chemistry. Background technique [0002] Solid phase microextraction (SPME) is a new type of sample pretreatment and enrichment technology that emerged in the 1990s. It was first proposed by Professor J. Pawliszyn of the University of Waterloo in Canada in 1989. In order to improve the extraction capacity, after continuous A variety of new SPME devices have been developed, among which in-tube solid phase microextraction is the most researched and applied one at present. This method has a larger extraction specific surface area and thinner extraction phase coating, high enrichment factor, and desorption It is easy to attach and easy to realize a...

AI Technical Summary

Problems solved by technology

In particular, methacryloyloxyethyltrimethylammonium chloride is used as a bridge, which not only combines with graphene oxide through π-π stacking, but also combines with titanium dioxide through electrostatic interaction, and its methacrylic structure can be combined with Solution polymerization of monomer and cross-linking agent, so that the titanium dioxide complex grows in the monolithic column structure, and successfully prepares the in-tube solid phase microextraction column, which solves the problem that the titanium dioxide monolithic column is not easy to prepare

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